Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Human stem cell"”.
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Defining and Controlling the Subtype Identity of Human Stem Cell-Derived Motor Neurons
One cardinal promise of stem cell research is that many intractable, common, and poorly understood diseases may be studied in an entirely new way: in vitro in the specific human cell types affected in vivo. Embryonic stem (ES) cells have the pluripotency to generate all somatic cells types, and the …
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Islamic Bioethics: National Regulations and Guidelines of Human Stem Cell Research in the Muslim World
<p>The utilization of human stem cells emerged recently in the Muslim world as one of the essential valuable areas of medicine for their vital role in developing regenerative medicine and treating chronic and incurable diseases. Existing studies indicate that most human stem cell researchers rely …
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Traumatic Injury-Induced Cell Responses in Human Cortical Organoids
… This is driven by secondary injury cascades of cellular toxicity, inflammation, and aggregation of toxic proteins. Although animal models and reductionist human cell culture systems provided important mechanistic insights, there is no available therapy to prevent pathological progression, which …
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RNAi-based logic circuitry coupled to Cas9-mediated transcriptional control in human cells and applications to stem cell technology
… core genetically encoded in the genome of human induced pluripotent stem cells (hiPSCs). This architecture can be theoretically programmed to precisely control stem cell differentiation. First, we use computational biology to analyze differential miRNA expression during human stem cell …
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ESTABLISHMENT IN CULTURE OF MOUSE AND HUMAN STEM CELLS WITH EXPANDED FATE POTENTIAL
… it has not been possible to establish stable cell lines that resemble these totipotent-like cells. In this work, I hypothesised that by modulating signalling pathways known to be important in early embryonic development it may be possible to capture in vitro a self-renewing state that …
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Investigating Cell-Type-Specific Vulnerability in Mitochondrial Disease Using Stem Cell-Derived Neuronal Models
… remarkable tissue specificity, with certain cellular populations showing heightened susceptibility. Neurological involvement is among the most consistent features in these disorders, yet the molecular and metabolic drivers of selective vulnerability in different neuronal subtypes remain …
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Investigation of genetic PIK3CA activation in genome-edited human pluripotent stem cells
… targeted therapy and evidence for tissue- and cell lineage-specific distribution of PIK3CA mutations in PROS, developmental models of this disease will be a key asset for preclinical drug testing and for a better understanding of PIK3CA activation in development. This PhD project addressed the …
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An in vitro model of human yolk sac and epiblast crosstalk
… embryo culture techniques have recently allowed human embryos to be cultured in vitro past the point at which they would implant in vivo, until the internationally accepted culture limit of 14 days. Nonetheless, the reliance on surplus in vitro fertilised (IVF) embryos, along with the ethical and …
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Quantitative phase imaging: advances to 3D imaging and applications to neuroscience
… network has been demonstrated using the SLIM system. The emergent self-organization of a neuronal network in a developing nervous system is the result of a remarkably orchestrated process involving a multitude of chemical, mechanical and electrical signals. Little is known about the dynamic …
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Cell Fate Decisions in the Early Mammalian Embryo
… from the totipotent zygote via two successive cell fate decisions in the appropriate sequence, position and proportion, to generate a blastocyst capable of implantation and further development. In mammals this process has long been thought to be regulative, with cell-cell interactions flexibly …
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Phenotypic characterisation of human iPSC neuronal models of GM2 gangliosidoses
… the outer leaflet of the plasma membrane of many cells, especially neurons. Their functions are broad and varied but their high abundance in neurons leaves these cells especially vulnerable to the effects of their accumulation in ganglioside lysosomal storage disorders (LSD). The GM2 …
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CHROMATIN DISRUPTION IN HUNTINGTON'S DISEASE ALTERS DEVELOPMENT AND PRECEDES NEUROPATHOLOGY
La Corea di Huntington (MH) è una malattia neurodegenerativa causata da un’espansione del tratto CAG nel gene Huntingtina (HTT), che porta alla formazione di un tratto di glutammine eccessivamente lungo nell’HTT mutata (mHTT). Sebbene la manifestazione clinica si osservi generalmente in età …
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Human Stem Cells for Modeling Amyotrophic Lateral Sclerosis Disease Mechanisms and Modifiers
… a fatal neurodegenerative disease of the motor system. Although ALS has been extensively studied in post-mortem patient samples and animal models, there are currently no very effective treatments and there is no cure. One reason for the lack of treatment options in ALS may stem from the …
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Spatial Transcriptome Profiling of Gastrulating Primate Embryos
… postimplantation development, the pluripotent cells of the embryonic disc, the embryo proper, undergo gastrulation. This key process establishes the three germ layers, endoderm, mesoderm and ectoderm, which set the basis for all tissues emerging in the human embryo. Since postimplantation human …