Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Human epidermal keratinocytes"”.
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Alteration of differentiation and growth of normal human epidermal keratinocytes by benzo[a]pyrene and arsenic
Normal human epidermal keratinocytes (NHEK) were chosen as an in vitro model for mechanistic studies into how altered regulation of differentiation may play a role in the malignant transformation process in human cells. Initially, the cytotoxicity of four petroleum-derived hydrocarbons …
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Defining Gene Expression in Normal Human Epidermal Keratinocytes and Melanocytes: A Prerequisite for Understanding Hyperplastic and Neoplastic Pathology
… that study gene expression of melanocytes and keratinocytes in growth-activated or neoplastic skin diseases. Historically, mRNAs isolated from cultured epidermal keratinocytes or melanocytes are used to define normal gene expression patterns. In this study, we profiled global gene expression in …
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Tissue engineering of co-cultured skin substitutes using biocomposite membranes based on collagen and polycaprolactone
… by interaction with 3T3 fibroblasts, normal human epidermal keratinocytes (NHEK), and primary human epidermal keratinocytes (PHEK) and dermal fibroblasts (PHDF) in vitro respectively. The 1:20 collagen: PCL materials exhibiting good cell growth curves and mechanical characteristics were …
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An investigation into the mechanism of toxicity of zinc oxide nanoparticles.
… market has resulted in likelihood of exposure to human beings. The present study was aimed to assess the in vitro and in vivo interactions of ZnO NPs in the mammalian system and to elucidate the possible mechanism of their toxicity. Our in vitro results using human epidermal cells (A431), primary …
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Effects of arsenic and UVB radiation on induction of cyclin D1 in human keratinocytes.
… epithelial cancers. Arsenic metabolism in humans leads to its collection in the skin, where it encounters and can act with known carcinogen UVB. Epidemiological evidence of the two toxins demonstrates their potential to affect signaling pathways, with various consequences. Here we employ an …
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Investigation of pancreatic beta cell differentiation and function, and global profiling analysis of diabetes serum for the identification of disease biomarkers
… represented a possible route for generation of human pancreatic beta cells for the study of regulated insulin secretion. Attempts were made to generate iPS cells from the MiaPaCa2 pancreatic adenocarcinoma cell-line, normal human epidermal keratinocytes, and normal human limbal epithelial cells. …
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The role of miRNA-486-5p in hair growth and the hair follicle immune privilege
… dramatically decreased in alopecic skin in both humans and mice, in striking contrast to its prominent expression in the hair follicle (HF) epithelium of healthy anagen skin. Moreover, the expression of both pri-miR-486 and miR-486 is down-regulated in the human anagen HFs and keratinocytes …
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MicroRNA-184: A Multifaceted Regulator of Epidermal Keratinocyte Biology
… insults. Consisting of dynamic outer strata of keratinocytes that terminally differentiate from basal keratinocytes stem cells (KSC), the epidermis is maintained by constant spatial and temporal control. In psoriasis, a dysregulated and intensifying crosstalk between keratinocytes and immune …
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In vitro cytotoxicity and skin irritation testing of antimicrobial conjugated electrolytes : interactions with mammalian cells
… irritation testing using tissues derived from human epidermal keratinocytes. PPE-DABCO and PPE-Th were non-irritants up to the highest tested concentrations, 924 μg/mL and 100 μg/mL, respectively. The lack of skin irritation for all substances, as measured by two endpoints, alleviates initial …
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Adipose stromal cells enhance keratinocyte survival and migration in vitro, and graft revascularization in mouse wound healing model
… chondroitin-6-sulfate glycosaminoglycan, and an "epidermal" layer, which consists of silicone polymer. This silicone layer is removed after the collagen matrix is adequately vascularized (usually takes 2-3 weeks), and then a thin layer autograft is applied to the top of the neo-dermis. ASC are …
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Bedeutung von Interleukin-6, Oncostatin M und Interleukin-31 für die biochemische und immunologische Barrierefunktion der Haut
The barrier function of the human skin involves biochemical as well as immunological processes. The family of multidrug resistance-associated proteins (MRP) are efflux transporters which protect skin cells from xenobiotica and promote inflammatory defence responses against pathogens through the …
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Genomic Drivers of Cutaneous Squamous Cell Carcinoma Development
<p>Skin cancer is the most common malignancy in humans. Annually, in U.S. there are over 3 million cases with an estimated overall economic impact of $2 billion. Cutaneous Squamous Cell Carcinoma (cuSCC) comprises 15-20% of all skin cancers. cuSCC has the best-defined progression from a distinct …
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Heat stress: A risk factor for skin carcinogenesis
… alone. However, the effects of UVB plus heat on human epidermal cells have yet to be determined. Furthermore, the exact mechanisms responsible for the observed effects of heat stress need to be characterised in skin keratinocytes to increase knowledge of its risk in skin cancer. Heat stress …