Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 42 for “"Human dermal fibroblasts"”.
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Investigation on the Intracellular Sognal Transduction Mechanism of Human Dermal Fibroblasts of Photorejuvenation in Photodynamic Therapy
… of PDT on the signal transduction mechanism in human dermal fibroblast and the role of reactive oxygen species (ROS) in vitro. Normal human fibroblasts were treated with various sublethal and relatively low doses of 5-aminolevulinic acid (ALA) and red light. The inhibition of fibroblast …
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A Comparison of Cultured Human Dermal Fibroblasts Derived from Terminal and Vellus Hair Bearing Skin. Differences in the expression of inhibitors of apoptosis proteins, oestrogen receptors, and responses to oestradiol under normal and wound induced conditions
… hair follicles (small and unpigmented), while dermal fibroblasts from different anatomical regions also exhibit phenotypical differences. Tissue repair requires a tight control of cell proliferation, migration and apoptosis, and recent studies have shown the importance of inhibitors of …
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The roles of vitamin D in cutaneous wound healing: In vitro and ex vivo studies of the effect of 1,25(OH)2D3 and its precursors on human dermal fibroblasts and epidermal keratinocytes in cutaneous wound healing
In humans, the epidermis is the main site for the synthesis of Vitamin D3 (cholecalciferol) from 7-dehydrocholesterol. Cholecalciferol undergoes further hydroxylation in the liver and kidney to produce the active form of the circulating hormone 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3). In target …
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Behavior of deformed dermal fibroblasts seeded on a silicon membrane under high levels of strain
… morphological and functional abnormalities of human dermal fibroblasts as a response to deformation produced by a normal force that can lead to the potential formation of stretch marks in pregnant women, adolescents, and people with Cushing's syndrome. The main function of dermal fibroblasts is …
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Photo-biomodulation of human skin fibroblast sub-populations: a systematic approach for the optimization of optical treatment parameters
… treatment parameters in photobiomodulation of human skin fibroblasts. The project begins with an extensive analysis of 90 bibliographic reports in photobiomodulation published between 1985 and 2015, and revealed major inconsistencies in optical parameters selected for clinical applications. …
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Investigating the role of the Epstein-Barr virus-encoded oncoprotein, latent membrane protein 1, in cell transformation in vitro
Approximately 15–20% of all human cancers are associated with viral infections. Latent membrane protein 1 (LMP1) is the major transforming oncoprotein encoded by Epstein-Barr virus (EBV) and is known for its ability to transform B cells, epithelial cells and rodent fibroblasts in vitro. When …
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DIRECT REPROGRAMMING OF HUMAN FIBROBLASTS INTO CARDIOMYOCYTES
Direct reprogramming of human fibroblasts into induced cardiomyocytes (iCMs) offers a potential therapeutic approach for cardiovascular disease yet hampered by low efficiency of human iCM generation. Here, we established an optimal culture condition by defining a chemically defined media (CDM). In …
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TIS21 inhibits cell migration by downregulating expression of matrix metalloproteinases
… homolog of rat pheochromocytoma cell 3 (PC3) and human B-cell translocation gene 2 (BTG2). TIS21 regulates cell cycle progression and proneural genes expression by acting as a transcription coregulator that enhances or inhibits the activity of transcription factors. And In human tissue, BTG2 has a …
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Towards hES cell-based therapy of Epidermolysis Bullosa
… layer, epidermis) from pluripotent stem cells (human embryonic stem cells, hESCs). Undifferentiated hESCs were exposed to a complex microenvironment in organotypic cultures (to mimic conditions for epidermal stem cell development and maintenance); this yielded epidermis-like structures which …
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Use of fourier transform- infrared spectroscopic imaging as a tool for understanding early molecular events driving breast cancer progression
… breast tumor is a well-documented phenomenon. Fibroblasts are the cell type responsible for maintaining the stroma, and cancer-associated fibroblasts, characterized by the expression of α-smooth muscle actin (α-SMA) protein, are highly contractile compared with those found in normal tissue. …
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Surface Engineered Novel Patterned Polymers to Remove Pathogenic Biofilms from Human Skin. Effective Removal of Antimicrobial Resistant Bacteria from Chronic Wounds
… against either bacteria or primary cultures of human dermal fibroblasts using Water Soluble Tetrazolium Salts (WST-1) assay. High performance liquid chromatography analysis highlights low level of triclosan release from the PDMS. Bacterial infection in co-culture using the Boyden chamber assay …
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Modulation of human adipose-derived stem cells and dermal fibroblasts in co-cultures by activation of the IL-6 trans-signalling pathway via the soluble IL-6Rα receptor
… The aim was to evaluate the relationship between human dermal fibroblasts (DF), human adipose-derived stem cells (ADSC), IL-6 and activation of the IL-6 trans-signalling pathway under four different cell culture conditions. The two cell types were co-cultured to assess cell migration when …
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Stimulation of angiogenesis through growth factor delivery from synthetic heparinised hydrogels
… was measured using an XTT metabolic assay on human saphenous vein endothelial cells and human dermal fibroblasts. Neovascularisation was quantified in a subcutaneous rat angiogenesis model in which hydrogel growth factor combinations were implanted within porous polyurethane discs and analysed …
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Exploring the global gene expression programs and regulation in the response of quiescent human fibroblasts to distinct proliferative stimuli
Serum treatment of quiescent human dermal fibroblasts induces proliferation coupled with a complex physiological response that is indicative of their normal role in wound-healing. However, it is not known to what extent such complex transcriptional events are specific to a given cell type and …
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Investigation and control of dermal fibroblast signaling during injury repair
… regulates the injury contraction process in a dermal microtissue on a chip model. We found that a balance of tissue forces generated via RhoA activation is critical for injury contraction, and that spatially localized RhoA activation can recruit new cells to participate in injury contraction. …
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Nanoparticle effects on cell migration
… The first system that was examined in the human monocytes THP-1 cells. As a type of monocyte, THP-1 is known to migrate in response peptide formyl peptides. Another cell/chemoattractant pair that was examined was human dermal fibroblasts (HDF) and platelet-derived growth factor (PDGF). …
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Gradients in Collagen Films: Effects of Mechanical and Chemical Properties on Directional Cell Response
… dendrimer (IKVAV) attachment was tested using human dermal fibroblasts (HDFs), fibrosarcoma cells (HT1080s) and rat Schwann cells (RSCs). HDFs showed an unfavourable response to high EDC/NHS crosslinking levels, but increased proliferation with increasing genipin crosslinking. The presence of …
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Characterizing the Role of CDK2AP1 in Primary Human Fibroblasts and Human Embryonic Stem Cells
… the role of CDK2AP1 in somatic cells (primary human dermal fibroblasts (HDFs)) and human embryonic stem cells (hESCs) and specifically assess its impact on proliferation, self-renewal and differentiation. In the first part of this study, using a short-hairpin RNA (shRNA) approach, we …
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Interactions Between Host Cells And Merkel Cell Polyomavirus In The Context Of Infection And In Merkel Cell Carcinoma
… We performed MCPyV infections in primary human dermal fibroblasts and examined the interface between virus and host cell via several methods including qPCR, western blotting, immunofluorescence microscopy, qPCR, and lentiviral CRISPR knockout. We found that MCPyV infection leads to innate …
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Design, production and evaluation of an auxetic straining device for application in lung tissue engineering scaffolds
… biaxial straining device was then tested using human dermal fibroblasts seeded in collagen gels. It was found that, after 22 h of straining, the cells exhibited a significant increase in aspect ratio from 1.7 to 2.7 while maintaining a random orientation, indicating the biaxial nature of the …
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