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Showing 1 to 20 of 54 for “"Human Pluripotent Stem Cells"”.
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Modeling Monogenic Diabetes Mody3 Using Human Pluripotent Stem Cells
… diabetes has been challenging due to the lack of human model but also because mouse models do not recapitulate the disease. Here, we use human pluripotent stem cells (ESCs) and CRISPR-CAS9 (Clustered Regularly Interspaced Short Palindromic Repeat)/Cas9) nucleases to understand the most common form …
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Extracellular matrix based substrates for propagation of human pluripotent stem cells
In human pluripotent stem cell (hPSC) research and applications, the need for a culture system devoid of non-human components is crucial. Such a system should exhibit characteristics observed in conventional culture systems that have used mouse embryonic fibroblast feeders for hPSC self renewal …
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Efficient In Vitro Development of Photoreceptors from Human Pluripotent Stem Cells
… of the rod and cone photoreceptors in the human retina is among the most common causes of blindness. Replacing these damaged photoreceptors may help to restore vision. Repairing the damaged retina relies on the insertion of new, healthy cells. Embryonic stem (ES) cells and induced …
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Production of hepatocytes from human pluripotent stem cells for cell-based therapy
Human pluripotent stem cells (hPSCs) can self-renew indefinitely in vitro while maintaining the capacity to differentiate into diverse cell types, including hepatocytes. Thus, hPSCs allow for large-scale production of hepatocytes necessary for cell-based therapy. Importantly, cell-based therapy …
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Investigation of genetic PIK3CA activation in genome-edited human pluripotent stem cells
… This PhD project addressed the lack of human, developmental PROS models by establishing isogenic series of human induced pluripotent stem cells (iPSCs) with endogenously expressed, activating PIK3CA mutations. This involved the optimisation of a CRISPR/Cas9 protocol for efficient knockin …
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The use of human pluripotent stem cells to model HNF1B-associated diabetes
… nuclear factor 1B (HNF1B), result in multisystem disease including diabetes due to beta-cell dysfunction and pancreatic hypoplasia. However, the mechanisms that underlie development of diabetes in HNF1B mutation carriers are still not fully understood due to lack of an appropriate model …
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Activin/Nodal Signalling Controls the Epigenome and Epitranscriptome of Human Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) are an invaluable model for cellular and developmental biology, and hold great potential for translational applications. While great progress has been made in elucidating the signalling pathways regulating pluripotency and differentiation, our mechanistic …
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The Derivation of the Myocardial, Epicardial and Endocardial Lineages from Human Pluripotent Stem Cells
The adult heart is composed of cells from three major lineages: the myocardium, the epicardium and the endocardium. The myocardium is the working muscle and comprises primarily the ventricles and atria. The epicardium represents the outer epithelial layer that surrounds the myocardium and plays a …
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Characterizing the Immunogenicity of Islet-like Cells derived from HLA-edited Human Pluripotent Stem Cells
… much hope for the therapeutic use of islet-like cells generated from human pluripotent stem cells, important challenges limit the clinical translation of this technology. In this thesis, I describe my efforts to address two of these challenges: the inability to control the composition of the …
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O-linked N-acetylglucosamine in differentiation and gene expression of mouse and human pluripotent stem cells
… on the viability and pluripotency of mouse and human embryonic stem (ES) cells. Here we show that increased levels of O-GlcNAcylation in response to specific inhibition of O-GlcNAc hydrolase (Oga) hinder mouse ES cell differentiation. In addition to these findings, I could also demonstrate that …
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Characterization of the Effects of bFGF and GSK3β Inhibitors on Embryoid Bodies Derived from Human Pluripotent Stem Cells
… (EB) formation is a common first step in many human pluripotent stem cell (hPSC) differentiation protocols. Previous work suggests that EBs are sensitive to growth factor withdrawal if they are derived from hPSCs maintained in feeder independent media such as mTeSR1. To promote cell survival, …
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Forward programming of human pluripotent stem cells to a megakaryocyte-erythrocyte bi-potent progenitor population: an in vitro system for the production of platelets and red blood cells for transfusion medicine.
… demands and short shelf life. Our lab uses human induced pluripotent stem cells (iPSCs), as an attractive alternative supply, as iPSCs can be cultured indefinitely and differentiate into almost any cell type. Using a technique called forward programming, we over express three key …
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Derivation of lymphocytes from human induced pluripotent stem cells
Human pluripotent stem cells have the potential to produce essentially unlimited numbers of mature and functional blood lineage populations to study human hematopoiesis. Particularly, human induced pluripotent stem cells (hiPSCs) have the advantage to provide a source of autologous transplantable …
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Investigating the role of the Polycomb Repressive Complex 2 in human in vitro pancreatic differentiation
The potential to use human pluripotent stem cells in regenerative medicine is an idea that continues to excite and captivate much of the science community, decades after the initial discovery of human embryonic stem cells. This interest has grown with the discovery of induced human pluripotent stem …
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MICROGLIA-SUFFICIENT BRAIN ORGANOID
… development in animal models but their roles in human brain development remain poorly understood due to the lack of tissue access. The generation of brain organoids from induced human pluripotent stem cells (iPSCs) has been shown to recapitulate the human embryonic brain development process. …
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Materials and biological approach to gene delivery in human embryonic stem cells
… tool used in the study and manipulation of human pluripotent stem cells for regenerative medicine purposes. However current methods of transient gene delivery are still highly inefficient. Using materials and biologically based concepts, I aim to develop new methods and protocols to enhance …
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