Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Huh7"”.
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Regulation of Senescence Phenotypes by TIS21 gene in Huh7 Human Liver Cancer Cells
… 의해 유도되는 노화기전은 아직 정확히 규명되지 않았다. 본 연구에서 간암세포주인 Huh7 에 저농도의 doxorubicin을 이용하여 유도되어진 세포노화현상에 anti-proliferative gene 인 TIS21이 미치는 효과를 확인하였다. Huh7 세포에서 TIS21단독으로는 세포노화 과정에 관여하지만 흥미롭게도 Doxorubicin을 이용해 노화를 유도한 Huh7 간암세포에서는 오히려 Doxorubicin에 의해 유도되어지는 노화를 억제하는 결과를 보여주었다. 이를 몇 가지 대표적인 노화 표지들을 이용하여 …
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Induction of Cellular Induction Senescence by BTG2_(TIS21/PC3) Gene in Huh7 Hepatocellular carcinoma Cells
… (HDF) cells. Furthermore, when we treated Huh7 hepatoma cells with H₂O₂, the expression of BTG2 mRNA was highly induced, accompanied with senescence phenotypes. To investigate a role of TIS21 in the process of cellular senescence, Huh7 cells were transfected with TIS21 cDNA construct. The …
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Reduction of tumorigenesis in androgen receptor overexpressed Huh7 cells through TGF-β induced cellular senescence phenotypes
… the potential role of AR in hepatoma, Huh7-AR and Huh7-V cell lines were established by transfection of Huh7 hepatocelluar carcinoma (HCC) cells with human androgen receptor (AR) in pCMV5 and its empty vector, respectively. And their in vivo and in vitro tumorigenesis were evaluated in …
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Identification of a novel method for differentiating human monocytic cell line into macrophages
… a monocytic cell (THP-1) and a human liver cell (HUH7). The basis for choosing these two cells stemmed from research by Matak et al confirming their cellular communication. Aim: The aim of the research was to compare two different methods of THP-1 monocyte differentiation into M0 macrophages. An …
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Effect of TIS21 on DNA Damage Repair Induced by Etoposide
… H2AX after etoposide treatment in LacZ infected Huh7 cells were more retained than TIS21 infected Huh7 cells. Comet assay also showed that DNA damage induced by etoposide were smaller in TIS21 expressed cells. In order to examine TIS21 involved in DNA damage repair and apoptosis, immunoblot …
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Characterization of the antiviral activity of cyclosporine A against dengue virus
… DENV-2 in A549 lung alveolar epithelial cells, Huh7 human hepatoma cells, and THP-1 monocyte-derived macrophages. We demonstrated that CsA effectively inhibits DENV-2 in all three cell types. Notably, the antiviral activity of CsA against DENV-2 is independent of its target proteins, cyclophilin …
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Inhibition of Cell Proliferation through Regulated Intramembrane Proteolysis of CREB3L1
… ER stress. By comparing gene expression between Huh7 subclones that are permissive for hepatitis C virus (HCV) replication versus the non-permissive parental Huh7 cells, we identified CREB3L1 as a host factor that inhibits proliferation of virus-infected cells. Upon infection with diverse DNA and …
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AMP-activated protein kinase-mTOR signaling pathway and hepatitis B virus replication
… HepG2세포에서 유래한 HBV 가 증식하는 HepG2.2.15세포, PEB 세포와 Huh7세포에서 유래한 HBV 가 증식하는 Huh7 HBV WT stable 세포에서 AMPK의 발현과 인산화를 조사한 결과 세 가지 세포에서 AMPK의 인산화가 감소한 것을 밝혀냈다. HepG2.2.15세포, PEB세포와 Huh7 HBV WT stable 세포에서 mTOR 발현과 인산화가 증가하였다. HepG2.2.15 세포에서 AMPK의 인산화가 감소하였음에도 불구하고 regulatory-associated protein of …
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Viral and Host Genetic Determinants of Hepatitis C Virus Persistence and Interferon Resistance
… within a long-term culture system of Huh7 cell lines harboring different variants of an HCV genotype 1b subgenomic RNA replicon that differed only at two sites within the NS5A coding region. A replicon with a lysine (K) insertion at HCV codon 2040 (K2040) replicated efficiently and …
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Induction and Regulation of CXCL10 in Hepatocytes During Hepatitis C Virus Infection
… induction in a non-synergistic manner, and Huh7 human hepatoma cells expressing both receptors (TLR3+/RIG-I+ Huh7 cells) produced maximal CXCL10 during early HCV infection. Neutralization of type I and type III IFNs had no impact on virus-induced CXCL10 expression in TLR3+/RIG-I+ Huh7 cells, …
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The Role of FOXO3 in Mechanisms of Doxorubicin Resistance in Hepatocellular Carcinoma
… HCC as compared to treatment-naïve tumors. In Huh7 hepatoma cells, doxorubicin induced acetylation and S574-phosphorylation of FOXO3, and these modifications promoted doxorubicin-induced cell death by suppressing the pro-survival function of FOXO3. Resveratrol, an activator of SIRT deacetylase …
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Relationship between Hepatitis B Virus Core Particles and Intracellular Motility
… lines were established in hepatoma cell lines, HuH7 and HepG2 cells. Using Northern blot and Southern blot analysis, HBV transcription and replication were detected in HBV replicating stable cell lines. Distribution of intracellular organelles was elucidated by immunfluorescence staining. …
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Identifizierung und partielle Charakterisierung eines neuen humanen RNA-Bindeproteins, RBP138, und seiner Isoformen
… endogenem RBP138 gelang in der Hepatomazellinie Huh7 nur nach vorausgegangener Immunpräzipitation. In Lokalisationsstudien wurde für beide Isoformen eine cytoplasmatische, vorwiegend perinukleäre Verteilung beobachtet, die eine Assoziation mit intrazellulären Membranen oder Cytoskelettstrukturen …
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The Intracellular Changes and the Localization of Core particles by Hepatitis B Virus P Protein-Deficient Mutant
… stable cells had been established in HuH7 hepatoma cell lines. Using Northern blot and Southern blot analyses, HBV transcription and replication were examined in HBV replicating and non-replicating stable cells. Distribution of intracellular organelles was examined by immunofluorescene …
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Understanding the Role of the Hypervariable Region in the Open Reading Frame 1 of the Hepatitis E virus in Viral Replication
… in the human liver carcinoma cell line, Huh7, were examined. Replication levels of mutants carrying these deletions were compared with that of the wild-type HEV in Huh7 cells. We observed that deletions in the HVR did not abolish viral RNA synthesis but substantially reduced the …
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The Modulation of Innate Immunity by Cellular and Viral Noncoding RNAs
… results in higher virus infectivity in human Huh7 cells and human primary dermal fibroblasts. Transfection of sfRNA prior to DENV 2 infection inhibited type I and III interferon induction and signalling and enhanced viral replication. Therefore, we posit that sfRNA present in salivary …
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Charakterisierung von funktionellen Oberflächenstrukturen des Hepatitis-B-Virus Nukleokapsids
… den Ergebnissen des ersten Teils wurden stabile HuH7-Zelllinien etabliert, die konstitutiv die virale Polymerase und das Wildtyp (WT) Coreprotein bzw. die I126A Mutante des Coreproteins exprimierten. Ein mittels Transduktion von rekombinanten MuLV-Retroviren in die Zellen eingebrachtes …
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Hepatitis B Virion and cccDNA Formation in Primary Tupaia Hepatocytes and Human Hepatoma Cell Lines upon HBV Genome Transduction with Replication-defective Adenovirus Vectors and in vivo Infection of Tupaias
… like the human hepatoma cell lines HepG2 and HuH7, are efficiently transduced and produce all HBV gene products required to generate the secretory antigens HBsAg and HBeAg, replication-competent nucleocapsids, and enveloped virions. It was further demonstrated that covalently closed circular …
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Systematic analysis of the role of differential expression of microRNAs associated with cell death decisions
… in the hepatocellular carcinoma (HCC) cell line Huh7, microRNA expression changes resulting from treatments with different combinations of the cytokines IFN-[gamma] and TRAIL follow a time-dependent pattern that correlates with cell death. An initial stimulus with IFN-y, followed by a second …
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