Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Hsf1"”.
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THE ROLE OF HSF1 PROTEIN REGULATION ON NEURODEGENERATION
… activated heat shock transcription factor 1 (HSF1) via Hsp90 inhibition, is being investigated as a therapeutic option for proteinopathic diseases. HSF1 is a master stress-protective transcription factor which activates genes encoding protein chaperones (e.g. iHsp70) and anti-apoptotic …
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Hsp70-Mediated Regulation of Hsf1 Transcriptional Activity In Saccharomyces Cerevisiae
… by the transcription factor heat shock factor 1 (HSF1) during exposure to proteotoxic stress. HSF1 controls the basal and stress-induced expression of molecular chaperones and other protective targets. Dynamic regulation of HSF1 involves the major heat shock proteins Hsp70 and Hsp90. Recent …
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Investigation of Riluzole’s Synaptic Protection Mechanism Through HSF1-BDNF Axis
… via focus- ing on the Heat Shock Factor 1 (HSF1)-dependent mechanisms. We found that Riluzole could increase HSF1 and BDNF (Brain-Derived Neurotrophic Factor) expression both at transcriptional and translational levels. CA1 (Cornu Ammonia, the first region in the hip- pocampal circuit), is …
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Leveraging HSF1 chemical-genetic tools to elucidate mechanisms of proteostasis
… transcriptional regulator Heat Shock Factor 1 (HSF1), which induces expression of cytosolic chaperones in response to proteotoxic stressors. Previous work in our lab enabled precision regulation of HSF1 activity through constitutively active or constitutively dominant-negative variants. My …
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Regulation of transcription factors NRF2 and HSF1 in mediating cellular stress responses
… The HSR, mainly mediated by Heat Shock Factor 1 (HSF1), protects the integrity of the cellular proteome through the upregulation of a battery of genes involved in ameliorating proteotoxicity. Several small molecule activators of the KEAP1/NRF2/ARE pathway have been shown to activate HSR through …
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Mitochondrial respiratory defects induces claudin 1 expression via ROS-mediated HSF1 activation
… factor binding site Heat Shock Factor1 (HSF1), which are known to be regulated by intracellular redox status. Subsequently, we found, that HSF-1 binds to the Cln- 1 promoter. HSF1 knockdown using siRNA decreased Cln-1 expression and invasive activity in SNU449. These results suggest that …
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Dual Regulation of Telomerase Activity By HSF1 And Its Role in Prostate Cancer Progression
… and tumor progression have not yet been defined. HSF1, the primary mammalian heat shock protein transcription factor, may affect telomerase activity and transformation by regulating the expression of several hsp90 chaperone complex proteins in response to stress as well as regulating the …
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Chemical Biology Approaches to Interrogate Heat Shock Transcription Factor 1 Regulation in Cancer
<p>Heat Shock transcription Factor 1 (HSF1) has long been recognized as the master regulator and signal integrator in the eukaryotic proteotoxic stress response. Revealed by recent discoveries in cancer, the functions of HSF1 have extended far beyond its canonical role in protein folding, further …
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Overexpression of Heat Shock Factor 1 Protects against Pathological Proteins in Neurodegenerative Diseases
… transcriptional factor, heat shock factor 1 (HSF1). HSF1 is inactive when in complex with HSP90 and becomes active once it is released from this interaction in the presence of misfolded proteins. Studies have shown that levels of multiple HSPs are reduced in the spinal cord of ALS patients, …
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Regulation of The Heat Shock Response By Thiol-Reactive Compounds In The Yeast Saccharomyces Cerevisiae
… by the highly conserved transcription factor HSF1, which recognizes target <em>hsp </em>genes by binding to heat shock elements (HSEs) in their promoters. In recent years, significant efforts have been made to identify small molecules as potential pharmacological activators of HSF1 that could …
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Alcohol alters the expression of Soluble N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptors (SNAREs) and spontaneous γ-Aminobutyric Acid (GABA) release...
… of the transcription factor heat shock factor 1 (HSF1), while HSF1 transcriptional activity had no effect on Vamp1 mRNA levels. We then investigated whether ethanol altered neurotransmitter release in cortical neurons, using whole-cell voltage clamp electrophysiology. We found that alcohol …
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Evaluating the Therapeutic Effect of an Hsp90 Inhibitor in Mouse Models of Alzheimer’s Disease
… Downregulation of heat shock factor 1 (HSF1) by either a pharmacological inhibitor or a virus-delivered shRNA abolished OS47720’s effects on memory improvement against Aβ toxicity. Overexpressing HSF1 partially improved memory. Together with the notion that HSF1-regulated BDNF mRNA, this …
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Controlled Myogenic Differentiation of Mammalian Cell Lines by Temperature-Inducible Systems
… overexpression of exogenous heat shock factor 1 (HSF1), a multifaceted factor that enhances Hsp70 transcription, regulates the localization of binding partners, and modulates translation under stress. Our results demonstrate efficient induction of exogenous pro-myogenic factors with a 2-hour heat …
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Redox Sensing By Yeast Hsp70 Facilitates Modulation of Protein Quality Control and The Cytoprotective Response
… and viability. Ssa1 binds to and represses Hsf1, the major transcription factor controlling the heat shock response, and the oxidomimetic Ssa1 failed to stably interact with Hsf1, resulting in constitutive activation of the heat shock response. Consistent with the in vitro findings, …
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Screens for non-eukaryotic initiation factor two alpha dependent activation of ATF4 translation
… HSR to show that the ESR acts as a suppressor of Hsf1 activity in aneuploid yeast. We show that the ESR is cytoprotective and that active Hsf1 cannot compensate for loss of the ESR. We also show that ESR activation in aneuploid yeast is cytoprotective. These studies highlight the complexity of …
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Advancements in genome engineering and applications in mammalian systems
… dimerizer was fused with activator domain p65-HSF1. Addition of inducers enabled dimerization which resulted in the recruitment of p65-HSF1 molecules to targeted promoter regions for gene upregulation. These three platforms were tested with high induction efficiency and were able to regulate …
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Regulation of Mitochondrial Function by Histone Acetylation and Mitochondrial Acetyl-CoA Levels in Saccharomyces cerevisiae
… shift factor Gis1 and the heat-shock factor Hsf1. In the first result chapter, the contribution of the three classes of TFs to the expression of chaperone reporters, antioxidant defence systems, storage carbohydrate accumulation, and chronological lifespan (CLS) extension were assessed. While …
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Mechanism of Rare Variant In Acta2, P.Arg149Cys, Driving Diverse Vascular Disease
… was also found to activate heat shock factor 1 (HSF1) in <em>Acta2<sup>R149C/+</sup></em> SMCs, which increases endogenous cholesterol biosynthesis through increased levels and activity of HMG CoA reductase. The increased cellular cholesterol levels lead to ER stress, thus engaging an UPR and …
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An Intermediate Heme Threshold is Necessary for the Activation of the Stress Response and Redox Homeostasis in Yeast
… by the transcription factors Msn2/4, Gis1 and Hsf1. Here, we reveal that an intermediate threshold of heme biosynthesis is sufficient to activate the stress response and maintain mitochondrial redox homeostasis but not mitochondrial respiration. In the first result Chapter (Chapter 3), using …