Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 8 of 8 for “"HoxB8"”.
-
Optimization and Validation of the Derivation and Use of HoxB8 Transgenic Murine Phagocytes in In Vitro Studies
… explored the use of another system that utilizes HoxB8 conditionally immortalized progenitor cells. The work presented in my thesis further explores the validation that conditionally immortalized phagocyte progenitors are comparable to those obtained through current methods which will ensure that …
-
MicroRNA targeting in mus musculus and Caenorhabditis elegans
… has conserved complementarity to the 3'UTRs of Hoxb8, Hoxc8, and Hoxa7; in particular, miR-1 96 has complete complementarity to Hoxb8 with the exception of a single G:U wobble. In 2004, Yekta et al., were able to detect RNA fragments diagnostic of miR-1 96-directed cleavage of Hoxb8 transcript …
-
An evolutionary perspective on the sequence, mechanism, and regulatory function of animal microRNAs
… diagnostic of miR-196-directed cleavage of Hoxb8 were detected in mouse embryos. Cell culture experiments demonstrated down-regulation of Hoxb8, Hoxc8, Hoxd8, and Hoxa7 and supported a cleavage mechanism for miR-196-directed repression of Hoxb8. These results point to a miRNA-mediated …
-
Dissecting the early steps of MLL induced leukaemogenic transformation using a new mouse model of AML
… on the mouse haematopoietic progenitor cell line Hoxb8-FL. Hoxb8-FL cells are able to proliferate indefinitely in vitro in the presence of Flt3 ligand (Flt3L) and an estrogen (β-estradiol)-regulated form of Hoxb8, and show lympho-primed multipotent progenitor (LMPP)-like differentiation capacity …
-
Molecular signatures underlying the tumourigenic transformation in a CEBPA mutated model of leukaemia
… version of the gene was introduced into the Hoxb8-FL cell line as cell of origin. The Hoxb8-FL cells are multipotent and can differentiate into dendritic cells with the supplementation of Flt3 ligand (Flt3L). However, Hoxb8-FL cells transduced with the CEBPA N321D construct failed to …
-
From Mechanisms of Leukaemogenic Dysregulation to New Candidate Therapeutics
… haematopoietic progenitor murine cell line (HoxB8-FL) allowing for the reproducible tracking of early leukaemic transformation and transcriptional changes due to MLL-ENL expression (Basillico., *et al* 2020). Using ME-Transformed cells, we knocked out 64 genes via CRISPR-Cas9 followed by …
-
Deciphering pre-leukaemic dysregulation caused by mutant C/EBPα at the level of the single cell
… mutation. The conditionally immortalised murine Hoxb8-FL cell line can differentiate into relatively homogenous populations of functional myeloid, lymphoid and dendritic cells in appropriate cell culture conditions. These cells were transduced with retroviral vectors which expressed either no …
-
THE ROLE OF THE STRESS RESPONSE GENE GADD45A IN MODULATING MYC MEDIATED APOPTOSIS AND DIFFERENTIATION
… of Myc expressing cells, the loss of Gadd45a in Hoxb8 generated cell lines shifted differentiation towards increased neutrophils. Determining the role of Gadd45a on the biological outcome of myeloid cells in response to deregulated c-Myc will provide vital information in understanding the …