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Showing 1 to 20 of 82 for “"Horseradish Peroxidase"”.
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Unusual catalytic properties of horseradish peroxidase
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Applied Biological Sciences, 1986.
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Studies of the chlorinating intermediate of horseradish peroxidase
The chlorinating intermediate of horseradish peroxidase, Compound X, is the only know stable enzymatic halogenating intermediate known. The optical spectrum of Compound X is virtually identical to the optical spectrum of Compound II. Titration of Compound X by a single electron donor, potassium …
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Inhibition of horseradish peroxidase activity by specific antibody
… characterize the antibody-mediated inhibition of horseradish peroxidase activity. Inhibition of enzyme activity was determined when alternate substrates were used. Typical biphasic patterns of inhibition were observed with each of five electron donors and three hydroperoxides. In no case was …
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Engineering a highly enantioselective horseradish peroxidase by directed evolution
… of the commercially useful enzyme, horseradish peroxidase (HRP), toward a chiral phenol. In doing so, we discovered that mutations close to the active site not only preserve HRP catalytic activity but impact its enantioselectivity far greater than distal mutations. We thus examined …
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Inhibition and inactivation of prostaglandin synthase-2 and horseradish peroxidase
… containing both cyclooxygenase (COX) and peroxidase (POX) active sites. Development of PGHS-2-selective POX inhibitors may reduce the toxic and carcinogenic effects of PGHS-2 xenobiotic activation, as well as further define the role of this activity in vivo . Knowledge of the contribution …
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The Free Radical Nature of Compound I From Horseradish Peroxidase and Chloroperoxidase
Compound Is were prepared from Horseradish Peroxidase (HRP), Chloroperoxidase (CPO), mesohemin substituted Horseradish Peroxidase (M-HRP) and deuterohemin substituted Horseradish Peroxidase. The Compound Is were analyzed by visible absorption, electron paramagnetic resonance (EPR), Mossbauer and …
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Evaluation of immobilizing horseradish peroxidase and alcohol oxidase in PVA-AWP polymer
… film thickness on the activity and stability of horseradish peroxidase (HRP) and alcohol oxidase (AOX) when immobilized in a photo-crosslinkable and water-soluble polymer, poly (vinyl alcohol) azide-unit water pendant (PVA-AWP). The immobilized enzyme films were to be used in biosensing …
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Application of additives in horseradish peroxidase-catalyzed removal of phenol derivatives from aqueous solution.
Additives can be used in the horseradish peroxidase (HRP) catalyzed polymerization of phenolic compounds to increase the enzyme turnovers by more than 100-fold. In addition to polyethylene glycol (PEG) and gelatin, some polyelectrolytes may also prove effective as additives. The HRP saving is …
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Studies on the internalization and intracellular transport of horseradish peroxidase in Chinese hamster ovary cells
Soluble horseradish peroxidase (HRP) is internalized by Chinese hamster ovary cells, a cell line of fibroblastic origin (Adams et al., 1982). We have confirmed this result by showing no inhibition of uptake in the presence of divalent cation chelators (EGTA Mg or EDTA), excess (19 mg/ml) yeast …
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Stabilization of Horseradish Peroxidase Using Epoxy Novolac Resins for Applications with Microfluidic Paper-Based Analytical Devices
… of various concentrations was found to stabilize horseradish peroxidase for times in excess of two weeks. A variety of microPAD fabrications, enzyme dispensing methods, and substrate delivery techniques were explored.</p>
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Studies on the Mechanism of the Chlorination Reactions Catalyzed by Chloroperoxidase and by Horseradish Peroxidase With Chlorite
Chloroperoxidase catalyzes the dismutation of chlorite-forming chloride, chlorine dioxide, chlorate, and oxygen as products. Chloroperoxidase also catalyzes the decomposition of chlorine dioxide. Chloride, chlorate, and oxygen are the products of the decomposition of chlorine dioxide. The optimum …
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THALAMIC PROJECTIONS TO MOTOR PREFRONTAL, AND SOMATOSENSORY CORTEX IN THE SHEEPSTUDIED BY MEANS OF THE HORSERADISH PEROXIDASE RETROGRADE TRANSPORT METHOD
… OF THEIR THALAMIC AFFERENTS TRACED WITH THE HORSERADISH PEROXIDASE METHOD. THE MOTOR AREA (AREAS 4 AND 6) OCCUPIES THE CRUCIATE GYRUS. IT RECEIVES A SUBSTANTIAL PROJECTION FROM THE THALAMIC NUCLEI VEΝTRALIS ANTERIOR, VENTRALIS, LATERALIS, MEDIALIS DORSALIS, AND CENTRALIS LATERALIS AND A …
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Investigation of the interaction of GdnHCl with poly-L-lysine, horseradish peroxidase and cytochrome c monitored via FTIR, CD and fluorescence spectroscopies
… with three model systems: poly-L-lysine (PLL), horseradish peroxidase (HRP) and cytochrome c. Probing the chemically denatured states of proteins by FTIR is a novel technique, which has only recently been reported. To aid in the interpretation of the FTIR results, fluorescence and CD were …
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Novel Potentiometric Enzyme Electrode Based on the Fluoride Ion-Selective Sensor.
… compounds is catalyzed by the enzyme horseradish peroxidase to release fluoride ions.
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Synthesis of analogues of nordihydroguaiaretic acid and their oxidative metabolism
… oxidation with silver oxide, and oxidation with horseradish peroxidase. Results are analyzed via HPLC and UPLC-MS. It is found that 1 does not autoxidize at pH 7.4 as NDGA does. Two products are produced during incubation of 1 in rat liver microsomes with UPLC-ESI(-)-MS results giving m/z of …
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Isothermal Titration Calorimetry Uncovers Substrate Promiscuity of Bicupin Oxalate Oxidase
… the product H<sub>2</sub>O<sub>2</sub> to the horseradish peroxidase-catalyzed oxidation of 2,2'-azinobis-(3- ethylbenzthiazoline-6-sulphonic acid) and by membrane inlet mass spectrometry. Coupled enzyme assays often confound data interpretation. Isothermal titration calorimetry (ITC) measures …
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Investigation of the absorption pathways of some poorly absorbed drugs using human intestinal (CAC0-2) cell monolayers
… as well as the vesicularly transcytosed horseradish peroxidase was also investigated. As expected, 2 mM apical La3+ increases transepithelial electrical resistance 1.S-fold and decreases mannitol permeability by 63.0 % ± 1.37 %. This inhibition was not repeated by other cations. Apical 2 …
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Enhanced chemiluminescent immunoassay for staphylococcal enterotoxin B in foods
… reaction. The reaction was catalyzed by horseradish peroxidase (HRPO) conjugated to immunospecific anti-SEB immunoglobulin G (IgG). Addition of $\rho$-iodophenol to the reaction mixture resulted in $>$2,000 fold increase in light emission over the unenhanced reaction. Furthermore, light …
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Methods to Induce and Synchronize Ovulation and to Enhance Corpus Luteum Function and Fertility in Beef Females (Postpartum Cows, Heifers)
… in beef females was developed using a horseradish peroxidase labelled progesterone conjugate and polysterene tubes. The sensitivity of the assay was 5 pg and 84% of the progesterone spiked to samples was recovered, which was similar to recovery of tritiated progesterone spiked samples …
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