Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Homologous Recombination Repair"”.
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Chemosensitization of Hepatocellular Carcinoma to Gemcitabine By Non-Invasive Radiofrequency Field-Induced Hyperthermia
… of gemcitabine incorporated in the DNA by repair mechanisms could potentially contribute to resistance in chemo-refractory solid tumors. In this study, we evaluated homologous recombination repair of gemcitabine-stalled replication forks as a potential mechanism contributing to resistance. …
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RNA and protein expression patterns of the Drosophila XRCC2 Homolog
… XRCC3/SpnB, and Rad51 C/SpnD participate in homologous recombination repair. The function of DmRad51 D and DmXRCC2 are unknown. The goal of this project was to elucidate the function ofXRCC2 in Drosophila. RNA interference allowed us to knockdown the function XRCC2 and its possible binding …
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The Fanconi anemia pathway and HELQ work alongside dormant replication origins to suppress replication-associated genome instability
… and hypogonadism, but not defects in homologous recombination repair. Rather, HELQ was found to work in parallel to the FA protein FANCC to suppress replication-associated genome instability.
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CRISPR/Cas9 screenings and in silico investigations nominate low-frequency alterations in DNA repair genes as biomarkers for castration-resistant prostate cancers.
… (mCRPC) patients characterized by defects in homologous recombination repair genes. Clinical trials results suggest that further improvements are possible, for instance in terms of patients’ enrolment criteria. Only 50% of BRCA1/2-deficient patients (TRITON2 trial) respond to therapy, variable …
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Biochemical and towards structural characterization of RAD51 and RAD51AP1 complexes
… breaks (DSBs). When unresolved or misrepaired DSBs can cause chromosomal breakages, fusions and chromosomal loss that drives cell death or genomic instability, which are hallmarks and drivers of cancer. The homologous recombination repair (HRR) pathway is an essential process that …
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Transcriptional and Chromatin Disorientation Associated with BRCA2 Inactivation
… is involved in genome integrity maintenance via homologous recombination repair, DNA replication fork protection, and R-loop resolution. However, the mechanism by which BRCA2 inactivation causes cancer remains unclear. In this study, using RNA-seq and ATAC-seq I investigated whether BRCA2 acts as …
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Functional investigation of the role of the retinoblastoma protein in genome stability
… of reactive oxygen species as well as impaired homologous recombination repair in cells with RB1 mutations. When xenografted into immune compromised mice, RB1 mutation also results in an increased capacity to seed new tumors in the lungs. This thesis also investigates the functions of the …
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Double-Strand Break Repair Mechanisms in Human Embryonic Stem Cells
… response. There are two major types of DSB repair; homologous recombination repair (HRR) and non-homologous end joining (NHEJ), both of which are regulated by members of the phosphatidyl-inositol-3’-kinase-related kinase (PIKK) family, including Ataxia Telangiectasia Mutated (ATM), Ataxia …
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Combination Chemotherapy and In vivo Modeling of BRCA-deficient, High-grade Serous Ovarian Cancer
… to the disease. Mutations in a number of homologous recombination repair genes have been implicated in hereditary ovarian and breast cancer. Specifically, over half of ovarian cancer cases exhibit BRCA-deficiency by either germ line or somatic mutation, or epigenetic silencing. The …
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Identifying New Genes for Inherited Breast Cancer by Exome Sequencing
… genes function in biological pathways related to homologous recombination repair. In order to identify additional mutations in these three genes, unrelated women with breast cancer or ovarian cancer and controls were evaluated with high-throughput targeted sequencing approaches including BROCA …
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The Importance of Dna Repair Capacity to (And A Model to Predict) Cell Radiosensitivity to Ions
… radiosensitivity to photons, including DNA repair capacity. Thus, it is expected that an additional benefit of using ions is that they mitigate the great heterogeneities in treatment responses commonly observed in photon therapies.</p> <p>However, by analyzing the cell survival of human …
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Leveraging genomic and molecular variations to understand the regulatory landscape in human cancers and differentiating stem cells
… signatures, using the cancer hallmark of DNA repair deficiency as an example. The molecular cause and consequence of homologous recombination repair deficiency are not yet fully understood. We explore genome-wide molecular aberrations caused by this repair deficiency beyond the few previously …
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Functional characterization of poly(ADP-ribose) dependent ubiquitin E3 ligase in the DNA damage response
… 1 A. DNA damage response 1 B. DNA damage repair 3 1. Homologous recombination (HR) 4 2. Nonhomologous end joining (NHEJ) 5 C. Ubiquitylation mediated DNA damage response signaling 6 1. Ubiquitylation 6 2. Post-translational modification mediated DNA damage response 7 3. RNF4 functions in …
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Targeting DNA Repair in Prostate Cancer: Therapeutic Combinations & Disease Models
Targeting prostate cancer cells with DNA repair inhibitors presents an opportunity to improve outcomes in patients with poor prognosis cancers. There are multiple reasons which rationalise targeting DNA repair in prostate cancer, including the enrichment of DNA repair genetic alterations in men …
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Identification of determinants of sensitivity to duocarmycin analogues: A class of potent anti-cancer drugs
… RAD51 foci formation in A549 cells. Moreover, homologous recombination (HR) deficient cell lines (BRCA2-/- in human colorectal cancer cell line and RAD51D-/- in CHO hamster cell line) showed hypersensitivity to treatment with duocarmycin analogues. The findings suggested that DNA alkylation is …
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Sviperfðabreytingar í Eggjastokkakrabbameinum
… finna vanvirkni í viðgerð tvístrendingsbrota (e. Homologous recombination repair) en meðal gena sem stuðla að viðgerðinni eru BRCA1 og 2. Vanvirknin getur stafað af ýmsum orsökum svo sem BRCA-stökkbreytingu í kímlínu, áunni (e. Sporadic) stökkbreytingu og hypermetýleringu á stýrisvæði BRCA1. …
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Dynamics of DNA-repair factors and chromosomes studied by laser-UVA-microirradiation and laser-photobleaching
… processes during the cell cycle and during DNA-repair. Laser-UVA-microirradiation The protein Rad51 is essential for the repair of double-strand breaks (DSBs) via the conservative homologous recombination repair pathway. To test the hypothesis that Rad51 localizes to damaged sites during DSB …
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Single molecule investigations into DNA replication, recombination and repair
… is a principal cytotoxic damage that can be repaired by several well-known repair pathways. One of the most prominent routes is the homologous recombination (HR). The mechanism of repair has been studied primarily by biochemical analysis over the years. Recent advances in single molecule …
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Investigation of a fusogenic pH-sensitive liposomal system for tumour-targeted intracellular delivery of dinitrobenzamide mustard prodrugs
… Chinese hamster ovarian cell lines, 51D1.3, its homologous recombination repair defective isogenic derivative 51D1 and 51D1 sPOR, which over-expressed a truncated form of the human NADPH:cytochrome P450 oxidoreductase (sPOR), known to be the major SN23816 reductase in hypoxic cells. Free SN23816 …
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Functional and evolutionary analysis of sugar transporters in Kluyveromyces marxianus
… Mutants were obtained in both yeasts both by non-homologous end- joining (NHEJ) and homology-dependent repair (HDR) methods, though the capacity to carry out allelic replacement by homologous recombination using repair fragments was of variable efficiency in different strains. To improve gene …