Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Homing endonuclease"”.
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Mobile genetic elements in Coxiella burnetii: Friends, foes or just indifferent?
… dnaB (encoding replicative DNA helicase) and a homing endonuclease. The introns are self-splicing ribozymes and able to inhibit ribosome function and retard bacterial growth through internal guide sequence (IGS)-dependent and -independent mechanisms. The introns were found to be highly conserved …
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Mobile genetic elements in Coxiella burnetii: Friends, foes or just indifferent?
… dnaB (encoding replicative DNA helicase) and a homing endonuclease. The introns are self-splicing ribozymes and able to inhibit ribosome function and retard bacterial growth through internal guide sequence (IGS)-dependent and -independent mechanisms. The introns were found to be highly conserved …
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Exogenously-introduced Homing Endonucleases Catalyze Double-stranded DNA Breaks in Aedes aegypti
… dengue fever, and dengue hemorrhagic fever. Homing endonucleases are selfish genetic elements which introduce double-stranded DNA (dsDNA) breaks in a sequence-specific manner. In this study, we aimed to validate a somatic assay to detect recombinant homing endonuclease (rHE)-induced dsDNA …
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DNA hydrolysis and genome editing applications of GIY-YIG family homing endonucleases
… Different classes of naturally occurring DNA endonucleases, including homing endonucleases, have therefore been explored as candidates for genome modification studies as they target long stretches of DNA. Homing endonucleases are mobile genetic elements whose biological role is to introduce …
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Harnessing Saccharomyces cerevisiae Genetics for Cell Engineering
… a robust in vivo DNA assembly method relying on homing endonuclease-stimulated homologous recombination. Reiterative Recombination elongates constructs of interest in a stepwise manner by employing pairs of alternating, orthogonal endonucleases and selectable markers. We anticipate that …
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Protein Engineering via in Vitro Coevolution
… coevolution was used to the engineer variants of homing endonuclease I-SceI with novel DNA cleavage activity. Homing endonucleases have great potential for applications such as targeted gene correction in gene therapy and gene alteration in systems biology and metabolic engineering. However, the …
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Improving Viral Vectors for Gene Targeting in Gene Therapy
… gene targeting has been achieved using the homing endonuclease I-Sce in AAV2, targeting has never been achieved using two zinc-finger nucleases (ZFNs) in any AAV serotype. This is significant because the recognition site for I-Sce is not found in the human genome, while ZFNs are designed to …
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Discovering and Characterizing New Homing Endonucleases for Genome Engineering
LAGLIDADG Homing Endonucleases (LHEs) are a family of highly specific DNA-cutting enzymes capable of recognizing target sequences of ~20 bp. In many eukaryotes, including humans and yeast, double-strand breaks induced by LHEs stimulate repair by Homologous Recombination, which can be used to alter …