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Showing 1 to 16 of 16 for “"Holliday junction"”.
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Characterisation of potential Holliday junction resolvases in Trypanosoma brucei
… a promising opportunity to treat the infection. Holliday junctions are an essential part of homologous recombination, which is used for DNA repair and for the crossover of DNA. Identifying T. brucei proteins which act as Holliday junction resolvases are potential candidate targets for inhibiting …
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The role of Bacteroides Host Factor A in CTnDOT integration and Holliday junction resolution
… The first set of strand exchanges generates a Holliday junction (HJ) intermediate that isomerizes through an overlap region and which is resolved by a second set of strand exchanges. Most tyrosine recombinases require identical DNA sequences in the overlap region where the strand exchanges …
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Solving the crystallographic structure of the Cl2J construct and occupancy titration trials to quantitatively determine isomer ratio
… the study of halogen bonds is the 4-stranded DNA Holliday junction. Incorporating engineered halogen bonds into the structure results in halogen bonds competing energetically against hydrogen bonds for stabilization of the junction. The structure that was refined in order to analyze chlorinated …
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Characterization of the Integrase of the Bacteroides Mobilizable Transposon NBU1
… system. I used nicked attBT1-1 substrates and a Holliday junction trapping peptide to show that NBU1 integration proceeds via formation of a Holiday junction intermediate that is formed by exchange of bottom strands. Some mismatches next to the first strand exchange site (in reactions with C(-3)G …
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Modeling and Simulating Biomolecular Machineries at Atomic Scale and Beyond
… tetramerical central pore; (3) a four-way DNA junction (Holliday junction), a central intermediate in homologous recombination, for which we have constructed a minimum geometric model and deduced a schematic framework of its conformer transition. The studies have been conducted mainly by …
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Analysis of Structure-Function Relationships in *Lambda and HK022 Site -Specific Recombination
… Int mutants were characterized that resolved Holliday junctions with biases different from the wild-type Int. The T168N and E153K substitutions may directly affect cooperative interactions between Int proteins bound to the Holliday junction intermediate. The V175E substitution appears to …
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Fluorescence-force spectroscopy at the single molecule level
… conformational transitions of the DNA four-way (Holliday) junction; 2) to dissect protein diffusion and dissociation mechanisms on single stranded DNA, 3) to calibrate FRET-based in-vivo force sensors and 4) to study mechanical unfolding of single proteins. The results could not have been …
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5-hydroxymethylcytosine and endonuclease G as regulators of homologous recombination
… 5hmC forms a unique hydrogen bond to stabilize Holliday junctions, the four-stranded DNA intermediate in HR. 5hmC also induces a global structure change to the junction, increasing protein access to the junction crossover and providing potential for either direct or indirect readout of 5hmC. …
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Functional analyses of hTel2 and TRF2 deltaB: Insights Into the Role of a New DNA Damage Pathway and Homologous Recombination in Mammalian Telomere Function
… and XRCC3, a component of the RAD51C/XRCC3 Holliday junction (HJ) resolvase, suggesting the involvement of homologous recombation (HR) in the deletions. Consistent with a role for HR, TRF2AB induced the formation of t-loop-sized telomeric circles. These telomeric circles were detected in …
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Pathways of NHEJ at Dysfunctional Telomeres and Their Resolution
… mammalian cells appear to employ the Holliday Junction resolvase Gen1, which is capable of cleaving cruciform DNA structures that can be formed when telomeres fuse. The deletion of Gen1 from TRF1 null cells resulted in the accumulation of chromatin bridges and isochromosomes, …
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Transport properties of DNA nanostructures
… The idea came from the nature-occurring Holliday junction (HJ) which allows more than 2 DNA strands to be assembled. Multiple HJs can be combined with desired orientation to form complex 2- or 3-dimensional objects. One of the most popular methods that realize this concept is the DNA …
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The Role of Esc2 and DNA in Smc5/6-mediated Sgs1-Top3-Rmi1 Sumoylation
… to the formation of DNA intermediates such as Holliday junctions (HJ), which are processed by one of two mechanisms: resolution or dissolution. In double Holliday junction dissolution, Sgs1-Top3-Rmi1 (STR) complex in yeast creates non-crossover products, and this process is regulated by …
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Genetic Analysis of Crossover Defective Mouse Spermatocytes Reveals Discrete Crossover Precursor Intermediates
… lacks a polymerized strand. The second, a double Holliday Junction (dHJ), requires the MutL homolog MLH3 in a nuclease-independent manner. We suggest that the dHJ is not fully ligated in mammals, unlike in yeast, where dHJ has been shown to be ligated. Finally, our evidence suggests that MLH3's …
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Characterization of the specificity and functions of the protein phosphatase Cdc14
… From this list, I identified the protein Yen1, a Holliday junction (HJ) resolvase, as a potential Cdc14 substrate. HJs are DNA intermediates that form during homologous recombination (HR) in response to repair of DNA double strand breaks. Yen1 has been shown to resolve HJs in a cell cycle and …
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Structure-function analysis of IntDOT, the CTnDOT integrase
… The first set of strand exchanges forms the Holliday Junction (HJ) intermediate and the second set of strand exchanges resolves the HJ to complete the recombination reaction. During formation and resolution of the HJ, the four IntDOT monomers communicate with each other and coordinate the …
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Acquisition and analysis of DNA recombination intermediates using single molecule FRET microscopy
Submitted by Meng Tao (mengtao2@illinois.edu) on 2012-10-23T20:45:46Z No. of bitstreams: 1 McKinney_Sean.pdf: 2035766 bytes, checksum: 414180e8b31f044c02a9ee30c58f9b8f (MD5)