Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Hoechst 33342"”.
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Somatic Stem Cell Populations and Studies on the Functional Role and Regulation of ABCG2
… chemotherapeutic drugs. Abcg2 can also efflux Hoechst 33342, resulting in a side population phenotype when stained cells are sorted by FACS. Recent studies have suggested that Abcg2 may be a marker for stem and progenitor cells. This paper presents experiments that were undertaken to further …
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Two -Photon Standing Wave Fluorescence Photobleaching With Application to the Study of Chromatin Motion
… labeled using the DNA minor groove binding dye Hoechst 33342 or GFP labeled histone H2B. The diffusion is highly constrained and cannot be observed in a standard, single beam fluorescence recovery after photobleaching experiment. To determine the chemical origin of the diffusion, we study motion …
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On the mechanisms of substrate translocation by the ABC transporter MsbA
… the major results related to the complexity of Hoechst 33342 described in Chapter 4 are published on Scientific Reports (2). 1. D. Guo et al., Energetics of lipid transport by the ABC transporter MsbA is lipid dependent. Communications Biology 4, 1379 (2021). 2. B. M. Swain et al., Complexities …
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Isolation and characterization of primitive hematopoietic cells
… rapid efflux of the fluorescent DNA-binding dye Hoechst 33342. Murine BM SP cells had long-term repopulating activity and proved negative for the antigen CD34. Such SP cells were detected in rats, pigs, monkeys and human cell sources. Within SP cells from human umbilical CB, CD7+/D34- lymphoid …
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Effects of a carcinogenic alkyl lesion on DNA: A structural study by x-ray crystallography
… complexed to minor groove binding drugs like Hoechst 33258, Hoechst 33342, Netropsin, as well as SN6999. In addition, the native dodecamer d(CGCGAATTCGCG) was crystallized with three drugs for comparison. The structures were solved by molecular replacement method and refined by constrained …
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Preclinical Pharmacology of the MDM2 Antagonist Nutlin-3a
… of BCRP substrates such as mitoxantrone and Hoechst 33342 in cells expressing functional BCRP without altering the expression level or localization of BCRP. Interestingly, nutlin-3b, considered virtually "inactive" in disrupting the MDM2/p53 interaction, reversed Hoechst 33342 efflux with the …
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Enhancement of death receptor-mediated apoptosis in multiple myeloma cells
… by assessment of nuclear morphology using Hoechst 33342/PI staining. TRAIL-resistant cells were generated by acute exposure of TRAIL sensitive cells to TRAIL followed by the selection of TRAIL-resistant cells (TRAILR). Apoptotic effects in quiescent cells (labelled as PKH26Hi) were also …
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Investigating the function of the multidrug transporter ABCG2
… a frequently used fluorescent ABCG2 substrate, Hoechst 33342, revealed that it was unsuitable for this purpose. Despite its efficient transport by ABCG2, the dye’s local pH can drastically affect its fluorescence when associated with lipid and DNA. Therefore, ethidium was favoured as a substrate …
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Study of porphyrins and heterodimers targeting ABCG2 transporter and development of a multiplexed flow cytometric approach for detection of anti SARS-CoV-2 IgG, IgM and IgA
… não alterou o efluxo de mitoxantrona e hoechst 33342 mediado por ABCG2. Por sua vez, o colesterol anula o efeito da chalcona já que o heterodimero chalcona-colesterol modificou o perfil inibitório quando comparado à chalcona sozinha. Estes resultados trazem novas ideias sobre o papel do …
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Assay Setup for High Throughput Screening to Identify Novel Efflux Pump Inhibitors against Escherichia coli
… E. coli -kannan efluksiaktiivisuus määritettiin Hoechst 33342 (H33342) -kumuloitumiskokeella, sekä ilman että yhdessä viiden EPIn kanssa. Samaa menetelmää hyödynnettiin annos-vastekokeissa, joissa EPIen optimaalinen annos selvitettiin. Matalin bakteerikasvua estävä lääkeainepitoisuus (MIC-arvo) …
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Charakterisierung humaner kurz-zeitrepopulierender Blutstammzellen im NOD/SCID-ß2m-/- Xenotransplantationsmodell
… die Ausbildung einer "Side Population" nach Hoechst 33342 Färbung in der durchflußzytometrischen Analyse lag diesen Experimenten zugrunde. CD3 depletierte SP und nicht-SP mPB Zellen wurden in Verdünnungsreihenexperimenten in insgesamt 105 NOD/SCID-ß2m-/- Mäuse intravenös transplantiert und …
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Intracellular signalling by arachidonic acid metabolites
… by condensation of chromatin (visualised after Hoechst 33342 staining), appearance of nucleosomal DNA fragmentation upon electrophoresis and flow cytometry analysis, and activation of caspase-3. Apoptotic cell death was inhibited in the presence of serum (0.5% w/v) and albumin, not serumderived …
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Phytochemistry and biological studies of constituents from Breonadia Salicina (VAHL) Hepper and J. R. I. Wood
… bromide) and Hoechst 33342/Propidium iodide (PI) dual staining assays. Eight compounds (bodinioside Q, 5-O-caffeoylquinic acid, D-galactopyranose, hexadecane, kaempferol 3-O-(2’’-O-galloyl)-glucuronide, lupeol, palmitic acid, and sucrose) were isolated for the …