Global ETD Search
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Showing 1 to 10 of 10 for “"Hoechst 33258"”.
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Digital frequency domain fluorometry and the study of Hoechst 33258 DYE-DNA interactions
… lifetime at every ""pixel"" of an image. The dye Hoechst 33258 has been used for many years in the study of DNA and DNA binding. However, the fluorescent properties of Hoechst 33258 are not well understood. The dye is highly quenched in aqueous solutions and becomes brightly fluorescent when bound …
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Investigating the binding mode of azide labelled derivatives of Hoechst 33258 by NMR, UV-Vis and IR spectroscopy
… of the archetypal minor groove binding ligand, Hoechst 33258, with the azide acting as a reporter functional group. Chapter 1 describes both the state of the art, and also what is unknown regarding the processes of association and dissociation of small molecules from dsDNA, and how this project …
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Ultrafast infrared spectroscopy investigation of DNA-ligand interactions
… the interaction of a well-established DNA stain, Hoechst 33258, across a set of different dsDNA sequences. In the following chapter, 2D-IR spectroscopyis applied again to study the interaction of three new types of hairpin polyamide ligands, screened across a set of different minor grooves. The …
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Crystallographic studies of interactions between ligands and DNA oligonucleotides
… Chapter Two describes nine crystal structures of Hoechst 33258 analogues with d(CGCAAATTTGCG)2 and d(CGCGAATTCGCG)2 oligonucleotides, and is divided into two sections. Section A includes seven structures with Halogenated Hoechst 33258 analogues that are potential agents in radiotherapy, …
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Zellzykluseffekte von Mitomycin C
… Durchflusszytometrie nach dem BrdU/Hoechst 33258-Ethidiumbromid Verfahren durchgeführt. Mit diesem Verfahren ist es möglich zwischen proliferierenden und nichtproliferierenden Zellen (Ellwart, Stunkel et al. 1981) zu unterscheiden und Verteilungen der Zellen in bis zu vier …
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Exploring the dynamic landscape of small molecule-DNA binding
… binding of an archetypical minor groove binder, Hoechst 33258, could be reliably extracted. For the binding of Hoechst 33258, these modes reveal details about the interactions underpinning the formation of complexes with both its target and a sub-optimal DNA sequences. The insights gained were …
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ATM-Dependent ERK Signaling in Response to DNA Double Strand Breaks
… (BrdU)- substituted DNA were treated with Hoechst 33258 followed by long wave-length UV (UV-A) (BrdU photolysis). BrdU photolysis resulted in well-controlled, dose-dependent generation of DSBs equivalent to 0.2 - 20 Gy of IR, as detected by pulse-field gel electrophoresis, accompanied by …
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The Effects Of Equine Spermatozoa/Oocyte Co-Culture On Spermatozoal Acrosome Reaction And Viability
… to incubate for 4.5 hours. A viability stain, Hoechst 33258, was added to the second portion. Three groups of six salt-stored oocytes were inseminated with stained spermatozoa. Spermatozoa and oocytes were allowed to co-culture for one minute. At one minute, three oocytes from each group were …
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Effects of a carcinogenic alkyl lesion on DNA: A structural study by x-ray crystallography
… complexed to minor groove binding drugs like Hoechst 33258, Hoechst 33342, Netropsin, as well as SN6999. In addition, the native dodecamer d(CGCGAATTCGCG) was crystallized with three drugs for comparison. The structures were solved by molecular replacement method and refined by constrained …
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Dynamics of DNA-repair factors and chromosomes studied by laser-UVA-microirradiation and laser-photobleaching
… nuclear DNA and staining with the live cell dye Hoechst 33258, the system can be used to introduce double-strand breaks and single-strand breaks in the irradiated spots. The response of Rad51 to microirradiation By use of laser-UVA microirradiation the localization of Rad51 at damaged sites …