Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 48 for “"Hoechst"”.
-
Digital frequency domain fluorometry and the study of Hoechst 33258 DYE-DNA interactions
… lifetime at every ""pixel"" of an image. The dye Hoechst 33258 has been used for many years in the study of DNA and DNA binding. However, the fluorescent properties of Hoechst 33258 are not well understood. The dye is highly quenched in aqueous solutions and becomes brightly fluorescent when bound …
-
Investigation of the microbial populations in the activated sludge of the Hoechst-Celanese wastewater treatment plant
The microbial populations in the Hoechst-Celanese activated sludge were examined. Heterotrophs, denitrifiers, sulfate-reducers, protozoa and filamentous bacteria were enumerated. Variations in microbial populations were compared with influent and effluent constituent concentrations, and with …
-
Investigating the binding mode of azide labelled derivatives of Hoechst 33258 by NMR, UV-Vis and IR spectroscopy
… of the archetypal minor groove binding ligand, Hoechst 33258, with the azide acting as a reporter functional group. Chapter 1 describes both the state of the art, and also what is unknown regarding the processes of association and dissociation of small molecules from dsDNA, and how this project …
-
Charakterisierung von Patienten mit Typ-1-Diabetes, die bis 2002/2003 mit täglich zwei Injektionen von Depot-Insulin Hoechst CR oder CS behandelt wurden
… zur Therapiezufriedenheit mit Depot-Insulin Hoechst® CR/CS bzw. der anschließend durchgeführten intensivierten Insulintherapie. Statistische Analyse: ANOVA, Kontingenz-Tafeln.Ergebnisse: Bei gleichem Manifestationsalter (28 15 vs. 26 15 J.; p = 0,56) waren Patienten unter Therapie mit …
-
Factors affecting the growth of Nostocoida limicola II and other filamentous microorganisms in activated sludge systems
… in the bulking activated sludge at the Hoechst Celanese wastewater treatment plant. First, futile attempts were made to isolate the filamentous bacterium, <i>Nostocoida limicola II</i>, from activated sludge for purposes of its characterization. Chemostat and batch experiments were also …
-
The antimalarial activity of Dicoma anomala and the chloroquine resistance reversing effects of Sclerocarya birrea on Plasmodium falciparum in vitro
… the Asteraceae and Sclerocarya birrea [(A. Rich) Hoechst. subspecies caffra (Sond.) Kokwaro], a member of the Anarcadiaceae families, are used widely in Africa for a variety of traditional treatments. In this thesis it has been shown that these plants possess in vitro pharmacological activities …
-
Ultrafast infrared spectroscopy investigation of DNA-ligand interactions
… the interaction of a well-established DNA stain, Hoechst 33258, across a set of different dsDNA sequences. In the following chapter, 2D-IR spectroscopyis applied again to study the interaction of three new types of hairpin polyamide ligands, screened across a set of different minor grooves. The …
-
Somatic Stem Cell Populations and Studies on the Functional Role and Regulation of ABCG2
… chemotherapeutic drugs. Abcg2 can also efflux Hoechst 33342, resulting in a side population phenotype when stained cells are sorted by FACS. Recent studies have suggested that Abcg2 may be a marker for stem and progenitor cells. This paper presents experiments that were undertaken to further …
-
Isolation and characterization of primitive hematopoietic cells
… rapid efflux of the fluorescent DNA-binding dye Hoechst 33342. Murine BM SP cells had long-term repopulating activity and proved negative for the antigen CD34. Such SP cells were detected in rats, pigs, monkeys and human cell sources. Within SP cells from human umbilical CB, CD7+/D34- lymphoid …
-
Effects of a carcinogenic alkyl lesion on DNA: A structural study by x-ray crystallography
… complexed to minor groove binding drugs like Hoechst 33258, Hoechst 33342, Netropsin, as well as SN6999. In addition, the native dodecamer d(CGCGAATTCGCG) was crystallized with three drugs for comparison. The structures were solved by molecular replacement method and refined by constrained …
-
Crystallographic studies of interactions between ligands and DNA oligonucleotides
… Chapter Two describes nine crystal structures of Hoechst 33258 analogues with d(CGCAAATTTGCG)2 and d(CGCGAATTCGCG)2 oligonucleotides, and is divided into two sections. Section A includes seven structures with Halogenated Hoechst 33258 analogues that are potential agents in radiotherapy, …
-
Analyzing GFP-tagged cytoskeletal protein colocalization in human carcinoma cells
… were fluorescently labeled in three channels-Hoechst for nucleus, Texas Red phalloidin for actin, and GFP for protein-and images were captured using Cellomics scanning microscopy. After collecting data and testing software applications, we analyzed our data with Definiens software and …
-
Unique localization of protein kinase C isozymes in T51B rat liver cells and their role in proliferation and apoptosis.
… by apoptosis, as determined by flow cytometry, Hoechst staining and TUNEL assay. Furthermore, flow cytometric analyses indicate that as the cells approach confluence, the percentage of apoptotic cells increases with a corresponding decrease in the percentage of the G2 cell population. The role …
-
Effect of copper and nickel on the performance of an activated sludge system treating cellulose acetate wastewater
… influent to the activated sludge process at the Hoechst Celanese cellulose acetate manufacturing plant, located at Narrows, Virginia. The influent wastewater to the experimental unit was pretreated with lime and then selected amounts of copper and nickel were added separately or together to …
-
Zellzykluseffekte von Mitomycin C
… Durchflusszytometrie nach dem BrdU/Hoechst 33258-Ethidiumbromid Verfahren durchgeführt. Mit diesem Verfahren ist es möglich zwischen proliferierenden und nichtproliferierenden Zellen (Ellwart, Stunkel et al. 1981) zu unterscheiden und Verteilungen der Zellen in bis zu vier …
-
Exploring the dynamic landscape of small molecule-DNA binding
… binding of an archetypical minor groove binder, Hoechst 33258, could be reliably extracted. For the binding of Hoechst 33258, these modes reveal details about the interactions underpinning the formation of complexes with both its target and a sub-optimal DNA sequences. The insights gained were …
-
Phosphonium-functionalised gold nanoparticles for mitochondria targeted therapeutics.
… followed by irradiation, this was confirmed by Hoechst and caspase-3 staining. Quantification studies of phosphonium-functionalised gold nanoparticles by ICP-MS illustrated that these nanoparticle have good uptake in cells (above 75%). TEM data confirmed that phosphonium-functionalised gold …
-
ATM-Dependent ERK Signaling in Response to DNA Double Strand Breaks
… (BrdU)- substituted DNA were treated with Hoechst 33258 followed by long wave-length UV (UV-A) (BrdU photolysis). BrdU photolysis resulted in well-controlled, dose-dependent generation of DSBs equivalent to 0.2 - 20 Gy of IR, as detected by pulse-field gel electrophoresis, accompanied by …
-
Plant-Pathogen Interactions Associated with Wasting Disease in the Tropical Seagrass Thalassia testudinum.
… Detection of nuclear condensation by TUNEL and Hoechst staining were also inconclusive and showed diffuse genetic material throughout the cytoplasm. It appears as though lesions from Labyrinthula spp. infection are likely to be a direct result of pathogen-based damage as opposed to host PCD.
-
Einfluß von PTK787/ZK222 584, einem spezifischen Rezeptor-Tyrosinkinase-Inhibitor, auf Anatomie, Dichte, Permeabilität und Blutfluß in Tumorgefäßen im murinen Nierenzellkarzinom
… Mit Hilfe der Fluoreszenzfärbung mit Hoechst H33342 konnte die Dichte der Mikro- und Makrogefäße und ihrer Kaliber dargestellt werden. Die anti-tumorale und anti-angiogene Wirkung des Rezeptortyrosinkinase-inhibitors PTK787/ZK222 584 konnte reproduziert werden. Die dopplersonographisch …
Page 1 of 3