Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 155 for “"Histone modifications"”.
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Histone Modifications in Trypanosoma Brucei
… at inactive ES. In other organisms, the role of histone posttranslational modifications (PTMs) in influencing transcriptional states has been well-documented. To begin to understand the role of chromatin structure in the regulation of gene expression in trypanosomes, I developed a protocol for …
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Histone modifications and the Arabidopsis thaliana circadian clock
… or involved in light responses. Rhythmic histone acetylation has been demonstrated in the promoter of TIMING OF CAB EXPRESSION1 (TOC1). Here, I used semi-quantitative Reverse Transcriptase Polymerase Chain Reaction (semi-quantitative RT -PCR) to investigate which enzymes are involved in …
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Colocalization between Akirin, the NuRD Complex, and Histone Modifications
… HDAC1, functions to remove acetyl groups from histone tails. Therefore, the colocalization between Akirin and histones modified by acetylation and methylation were also examined.</p> <p>A novel polytene squash method was developed as a byproduct of our study to improve the quantity of usable …
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SWI/SNF COMPLEXES COORDINATE WITH HISTONE MODIFICATIONS TO REGULATE CHROMATIN REMODELING
… to intercalate between the DNA gyre and the histone octamer at SHL2. Consistent with its DNA footprint, RSC however makes extensive contacts with both nucleosomal and extranucleosomal DNA through five major subunits Sth1, Rsc2, Rsc3, Rsc30 and Rsc4. Excepting the catalytic subunit Sth1 which …
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Patterns of post-translational histone modifications, chromatin condensation and DNA fragmentation during apoptosis
… von spezifisch modifizierten Nucleosomen/Histonen in den Kern oder ins Zytoplasma nach Apoptoseinduktion zeigen kontinuierlich ansteigende Konzentrationen aller vier Core Histon Typen (H2A, H2B, H3, H4), wobei bevorzugt die modifizierten Core Histone H3 di-/trimethyl K4, H3 dimethyl K9 und …
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Dynamic histone modifications at the promoters of Hox genes in embryonic stem cells
Histone modifications have been closely associated with changing levels of gene expression, but their role in determining, or possibly predicting, patterns of expression is uncertain. Here, the link between histone modifications and Hoxb gene expression in mouse embryonic stem (ES) cells was …
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New Microfluidic Technologies for Studying Histone Modifications and Long Non-Coding RNA Bindings
… without changing DNA sequences. Epigenetic modifications, such as DNA methylation, histone modification, and non-coding RNA interaction, play critical roles in cell differentiation and disease processes. The conventional approach requires the use of a few million or more cells as starting …
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Trim24-Regulated Estrogen Response Is Dependent On Specific Histone Modifications In Breast Cancer Cells
… activation is dependent on specific histone</p> <p>modifications in tumorigenic human breast cancer-derived MCF7 cells. In the first</p> <p>part, I proved that TRIM24-PHD finger domain, which recognizes unmethylated</p> <p>histone H3 lysine K4 (H3K4me0), is critical for ERα-regulated …
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Analysis Of The Role Of Amphetamine In The Induction Of Changes In Histone Modifications Of Dopaminergic Pathway Genes
… that repeated cocaine administration changes histone acetylation patterns at the promoter sequences of several important genes. Sirtuin proteins are histone deactylases that regulate important biological functions have also been found to be elevated in the nucleus accumbens after cocaine …
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Methodology development for imaging histone modifications and for site-specific protein labeling in vitro and on the surface of living cells
… methodology for monitoring post-translational modifications of histone H3 in living cells was developed using genetically encoded fluorescent reporters. These reporters were constructed for sensing histone phosphorylation and methylation by fluorescence resonance energy transfer (FRET). These …
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DISCOVERY OF NOVEL EPIGENETIC MECHANISMS OF CARCINOGENESIS BY GENOME-WIDE PROFILING OF NON-CODING REGULATORY ELEMENTS
… events. Far less is known about the role of histone modifications in epigenetic mechanisms of human cancers, and of disease in general. There are a number of reasons why histone modifications are especially important to investigate in the context of tumor formation. While DNA methylation …
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Elucidating the regulation of the Yeast Metabolic Cycle through the integration of gene expression and chromatin status
… phases and have described the effect of certain histone modifications in gene regulation. This project constitutes a novel application of different statistical methodologies for the integration of chromatin status data (ChIP-Seq) and gene expression data (RNA-Seq) to better understand the YMC. …
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Reproducibility and reliability of chromatin immunoprecipitation in clinical research
<p>Association between histones and DNA is crucial for many cellular functions such as gene transcription and epigenetic silencing. Changes to chromatin structure influence gene expression via histone modifications. Chromatin immunoprecipitation (ChIP) is an experimental technique used to …
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Integrating Epigenetic Priors For Improving Computational Identification of Transcription Factor Binding Sites
Transcription factors and histone modifications play critical roles in tissue-specific gene expression. Identifying binding sites is key in understanding the regulatory interactions of gene expression. Nave computational approaches uses solely DNA sequence data to construct models known as Position …
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Definition of The Landscape of Chromatin Structure At The Frataxin Gene In Friedreich’S Ataxia
… A number of studies demonstrated that specific histone deacetylase inhibitors (HDACi) affect either histone modifications at the <em>FXN</em> gene or <em>FXN</em> expression in FRDA cells, indicating that the hyperexpanded GAA repeat may facilitate heterochromatin formation. However, the …
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Molecular targets of chromatin marks H3K4ME3, H3K9ME3 and H3K27ME3 in an adult germinal niche
… in neural progenitors regulated by chromatin modifications. Neural progenitors of baboon SVZ were examined using ChIP-Seq (chromatin immuneprecipitation followed by deep sequencing) to determine genome wide gene targets of three histone modifications: H3K4me3, H3K9me3 and H3K27me3. Our data …
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Induced Gene Expression In Bladder Cancer: Kindlin-2 And Metallothionein-1x
… transcription factor binding, as well as, histone modifications in the MT-1X promoter of metal-transformed cells and compare it to that of the non-transformed parent. In addition, the effect of the histone deacetylase inhibitor (HDACi) MS-275 on factor binding and histone modifications was …
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Rationale Entwicklung rekombinanter CHO-Zellen basierend auf Histonanalyse und RNA-Seq: Identifizierung und Verwendung stabiler Integrationsorte
… CHO-K1 genome. Using genome-wide analysis of the histone modifications H3K4me3, H3K9me3, and H3K27ac and their association with gene expression, 709 potentially stable integration sites with specific histone modifications were predicted. The enhanced green fluorescent protein (eGFP) gene was …
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The chromatin landscape of barley: gene expression, evolution and epigenetics
… the genic and genomic distributions of nine histone modifications.<br/><br/>Firstly, regions of HR and LR were identified and compared for gene evolution, expression and diversity. LR regions of the barley genome were found to be restrictive for gene evolution and diversity, but not gene …
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