Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 85 for “"Histone Modification"”.
-
Histone modification during the induction of tyrosine transaminase
"The induction phenomenon is a useful model for the study of mechanisms which regulate the synthesis of specific enzymes in mammalian tissue, as well as for the role of hormones in this process". The in vivo administration of hormone initiates a chain of reactions which culminate in the increase of …
-
The Novel Regulation of Histone Modification In Cancer Development
<p>Dynamic changes in histone acetylation by various physiological cues play important roles in gene transcription and cancer. However, the cellular signaling underlying this regulation is not well understood. Here, we show that, in a glucose dependent manner, E3 ubiquitin ligase NEDD4 …
-
Histone modification and the epigenetics of X chromosome inactivation
… of ways, including the hypoacetylation of core histones, a common property of genetically inactive chromatin. This study has used Xi to explore the functional significance of hypoacetylation and patterns of histone methylation in silent chromatin. Xi was shown to be depleted for di- and …
-
Discovery and Characterization of Methylation of Arginine 42 on Histone H3: A Novel Histone Modification with Positive Transcriptional Effects
… turns of DNA wrapped around an octamer of core histone proteins composed of four histone species: one histone H3/H4 tetramer and two histone H2A/H2B dimers. Histones are basic globular proteins rich in lysine and arginine residues, with unstructured N-terminal "tail" regions protruding outside …
-
Computational algorithm development for epigenomic analysis
… developed for analyzing ChIP-seq datasets of histone modifications. For basic ChIP-seq data processing, the problems of ambiguous short sequence read mapping and broad peak calling of diffuse ChIP-seq signals were solved by novel statistical methods. Their performance was systematically …
-
Microfluidics for Low Input Epigenomic Analysis and Its Application to Brain Neuroscience
… cell-type-specific and spatially resolved histone modification profiling on microfluidic platforms. First, I applied Microfluidic Oscillatory Washing-based Chromatin Immunoprecipitation followed by sequencing (MOWChIP-seq) to study the effect of culture dimensionality, hypoxia stress and …
-
Microfluidic Technology for Low-Input Epigenomic Analysis
Epigenetic modifications, such as DNA methylation and histone modifications, play important roles in gene expression and regulation, and are highly involved in cellular processes such as stem cell pluripotency/differentiation and tumorigenesis. Chromatin immunoprecipitation (ChIP) is the technique …
-
THE APPLICATION OF HISTONE ANALYSIS FOR IDENTIFYING DISTAL REGULATORY ELEMENTS - REGULATION OF FSHR
… of chromatin structure and the role that histone modification plays in transcriptional regulation. In particular, these advances revealed important associations between functional, non-coding DNAs and specific histone modifications, which have been used technically to identify numerous …
-
The role of Polycomb-mediated epigenetic regulation in embryonic stem cell differentiation
… Complex 2 (PRC2) catalyzes the posttranslational histone modification H3K27me3, a histone modification associated with transcriptional repression. Although PRC2 has critical functions in lineage commitment and in mediating cell fate transitions, it has proved difficult to study its precise role in …
-
Theoretical and Computational Studies on the Dynamics and Regulation of Cell Phenotypic Transitions
… levels to name a few. Epigenetic histone modification plays an important role in cell phenotype maintenance and transitions. However, the underlying mechanism relating dynamical histone modifications to stable epigenetic cell memory remains elusive. Incorporating key pieces of …
-
Dynamic epigenetic modifications during human fetal germ cell development
… of normal germ cell development. Epigenetic modifications, which modify gene expression without changing the underlying DNA sequence, can affect the fate of germ cells. Abnormal epigenetic reprogramming will facilitate abnormal germ cell development; therefore, understanding of epigenetic …
-
Low-Input Multi-Omic Studies of Brain Neuroscience Involved in Mental Diseases
… syndromes are preserved by epigenetic modifications identified in several specific brain regions. In this thesis, we developed the second generation of one of our microfluidic technologies (MOWChIP-seq) and used it to profile genome-wide histone modifications in three mental …
-
DEFINING THE ROLE OF THE PCGF3-CONTAINING POLYCOMB REPRESSIVE COMPLEX 1 IN SYNOVIAL SARCOMA
… of the fusion oncoprotein for H2AK119ub, the histone modification deposited by Polycomb Repressive Complex 1 (PRC1). However, the precise molecular mechanisms underlying SS18-SSX transformation remain unclear. Given SS's disruption of epigenetic regulators SWI/SNF and Polycomb complexes, my …
-
From Protein Folds to Chromatin: Topological Data Analysis and Deep Learning at Multiple Scales
… CHROME predicts transcription factor binding and histone modification landscapes with high accuracy, generalizes across cell lines, and reveals how distal chromatin interactions influence local regulatory activity. Together, we bring a unified multi-scale approach that couples geometric, …
-
The effects of histone acetylation on the maize allele PL1-blotched
Covalent modifications of DNA and nucleosomal histone proteins associated with eukaryotic chromatin have the potential to alter expression of a gene without change in its DNA sequence. One gene regulated through this so-called epigenetic process is the Pl1- Blotched gene of maize. Pl1-Blotched is a …
-
Characterising the MLL complex: epigenetic regulation of Hoxa genes
… factor that acts to regulate genes via its histone methyl-transferase activity. This study aimed to examine how MLL1 and its associated complex contribute to gene regulation in a functionally relevant cell background. To assess dynamic processes involved, we developed a haematopoietic Stem …
-
Molecular mediators of cardiac-specific enhancer activation
… and integrated these data with maps of histone modification patterns and gene expression across several stages of embryonic stem cell (ESC) differentiation toward cardiomyocytes (CMs). Based on bioinformatic analysis of these data, we identified the TEA domain family (TEAD) TF TEAD1 as a …
-
EPIGENETIC REGULATION IN AN IPSC-NEURON MODEL OF FAMILIAL ALZHEIMER’S DISEASE
… in the brain. Dysregulation of epigenetic histone modifications may lead to the expression of transcriptional programs that may play a role in disease genesis or the worsening of disease pathology. One such histone modification, acetylation of histone H3 lysine residue 27 (H3K27ac), is …
-
Microfluidics for low input epigenomic analysis and application to oncology and brain neuroscience
… map RNA polymerase II, transcription factors and histone deacetylase binding in 1,000-50,000 cells. We examined mouse prefrontal cortex and cerebellum using this technology. We found extensive differences that correlated with distinct neurological functions of the brain regions. The same platform …
-
Define The Epigenetic Profiles and Subtype-Specific Genes of Breast Cancer
… wide profiling and global levels of eleven histone modifications localizing at promoter, gene body, and enhancer in distinct subtypes of breast cancer cell lines. With this knowledge, we will address how changes in chromatin modification landscape contribute to differential breast cancer …
Page 1 of 5