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Showing 1 to 18 of 18 for “"Histidine tag"”.
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EFFECT OF HISTIDINE TAG ON THE ENZYMATIC ACTIVITY OF PSEUDOLYSIN AND ITS INTERACTION WITH THE STREPTOMYCES METALLOPROTEASE INHIBITOR (SMPI) PROTEIN
… This study investigated the effect of N-terminal Histidine (His) tag on the enzymatic activity of PLN and its interaction with SMPI. His-tagged PLN and SMPI proteins were expressed in bacteria and purified by affinity chromatography and gel filtration. His tag was removed by digestion with tobacco …
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Soluble and Functional Overexpression of the Ligand Binding Domain of Mouse Aryl Hydrocarbon Receptor in <i>E.Coli</i>
… under native conditions with the use of 6 histidine tag but soluble overexpression was not possible. Thus a solubility enhancing tag called maltose binding protein (MBP) was used for purification of mAhR aa211-384 under native conditions, which still did not yield soluble overexpression. …
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The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants
… in Nicotiana benthamiana and purified using a 6x histidine-tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its ability to detect IgG antibodies against CCHFV in human sera.
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CHARACTERIZATION OF THERANOSTIC PEPTIDES FOR GLIOBLASTOMA MULTIFORME
… for periplasmic prokaryotic expression and histidine-tag purification was created. However, prokaryotic expression and purification of histidine-tagged rCTX was not obtainable. Confocal data also supports variable labelling activity of commercially sourced CTX and rBSG <em>in vitro</em> for …
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Molecular mechanisms of DNA repair in Mycobacterium tuberculosis
… of the DNA damage (SOS) response in adaptive mutagenesis, and focused on two specific components: the role of the specialist translesion synthesis DNA polymerase, DnaE2, in mutagenesis under stress and, secondly, the function of the mycobacterial homologue of a putative SOS response associated …
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Characterization of an altered MoFe protein from a nifV- strain from Azotobacter vinelandii
… of homocitrate in nitrogenase catalysis. A poly-histidine tail was incorporated into the C-termini of the a-subunit permitting isolation of the homocitrateless MoFe protein by using metal affinity chromatography. We have found that the addition of a poly-histidine tag does not alter the catalytic …
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Etude de l'interaction entre GpJ, une protéine du bactériophage Lambda, et LamB, une protéine de la membrane externe des bactéries gram-négatives
… de GpJ (684-1132) en fusion avec un 6×Histidine-tag. Cette protéine est exprimée sous forme de corps d’inclusion dans E.coli. Après purification et renaturation, une protéine de nouveau soluble peut être obtenue. Lors d’expériences de Film Noir, His-gpJ intéragit certes avec LamB, mais …
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Transition-Metal Complexes as Enzyme-Like Reagents for Protein Cleavage
… hydrolytic cleavage of the amide bond in histidine-containing dipeptides, has been studied. Transition-metal complexes are emerging as new reagents for selective cleavage of unactivated amide bonds in proteins. Such complexes bind to the side chains of methionine and histidine forming an …
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The Structure-Function Interface in the Cytochrome Bc1 Complex Family
… of an extra threonine residue between the histidine ligands of the two b-hemes in helix D resulted in modification of the spectrum and redox potential of the bL-heme. Mutations of Tyr-156, located close to the iron-sulfur cluster in the ISP to Leu, Phe or Trp led to alteration of the …
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Evaluation Of Immunogenicity Of Transgenic Chloroplast Derived Protect
… the pag (2205 bp) with the N-terminal 6X histidine tag, into the chloroplast genome by homologous recombination. Chloroplast integration of the pag was confirmed by PCR and Southern analysis. The PA83 protein was detected in transgenic chloroplasts by immunoblot analysis using anti-PA83 …
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Engineering intracellular antibody libraries
… correct size and could be purified using a poly histidine tag. Due to its bioaffinity and specificity, scFvs were constructed to profile disease patterns, and to identify potential drug targets. In addition to its original application to health-related studies, scFvs could also be extended to …
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A Study of the Membrane Binding Properties of the Matrix Protein from Human Respiratory Syncytial Virus
… The M protein was expressed in E. coli with a histidine tag and purified by immobilised metal affinity chromatography (IMAP). The protein was found to have acquired a mutation at position 254 from methionine to arginine, but this did not appear to affect its behaviour and both the native and …
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Regulating Inositol Biosynthesis in Plants: Myo-Inositol Phosphate Synthase and Myo-Inositol Monophosphatase
… cDNA was fused at the N-terminus to a 6X histidine tag (5' GAC GAC GAC GAC GAC GAC 3'), cloned into an overexpression vector and overexpressed in <I>E. coli</I>. The fusion protein, HISMIPS, was extracted using denaturing conditions and purified using Ni²⁺ affinity chromatography. …
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Characterization of two novel proteins containing the rhodanese homology domain: YgaP and YbbB of Escherichia coli
… YgaP is a cytoplasmic membrane protein. A polyhistidine-tagged variant of YgaP was subsequently solubilized from the membrane by detergent extraction and purified by metal chelate chromatography. Similar to the other characterized rhodaneses, purified YgaP-His6 as well as the …
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IDENTIFICATION AND ANALYSIS OF PROTEINS AND GENES RESPONSIBLE FOR MICROBIAL ANAEROBIC NITRATE-DEPENDENT IRON OXIDATION AND OVEREXPRESSION IN E. coli OF PERCHLORATE REDUCTASE
… overexpress perchlorate reductase and use a polyhistidine tag to enable ease of the subsequent purification. The pcrAB genes encoding the structural subunits of perchlorate reductase were cloned into an expression vector in E. coli. The purification of the recombinant perchlorate reductase was …
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Biosynthesis of the Nitrogenase FeMo-cofactor from Azotobacter vinelandii: Involvement of the NifEN complex, NifX and the Fe protein
… NifEN. In addition to gene fusion, a poly-histidine tag was incorporated into NifEN, allowing purification through the application of immobilized metal-affinity chromatography (IMAC). NifEN obtained in this way was characterized using a variety of biophysical techniques and found to contain …
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DEVELOPMENT OF CRISPR-CAS IMMOBILIZATION SRATEGIES FOR THE FABRICATION OF A BIOSENSING PLATFORM
… as a superior choice for its several advantages: their miniaturized format significantly reduces the amount of materials and samples required for analysis, while also offering high multiplexing capacity, enabling the simultaneous analysis of multiple targets in a single experiment. To …
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DNA enzymes for peptide-nucleic acid conjugation and for lysine methylation
… acid polymers, DNA offers additional advantages over RNA because DNA can be directly amplified by polymerases whereas RNA requires an extra reverse transcription step. DNA is also cheaper and more stable compared to RNA. An approach to site-specifically modify peptide and protein side …