Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 50 for “"Histidine Kinase"”.
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Targeting Receptor-Histidine Kinase Signaling in Staphylococcus Aureus
… signaling systems involving receptor-histidine kinases (RHKs) are present in all branches of life outside of the animal kingdom. Remarkably, very little is known concerning the extracellular ligands that presumably bind to RHKs to initiate signaling. The two-component agr signaling …
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Predicting and testing determinants of histidine-kinase functions by leveraging protein sequence information
… networks in bacteria is a pair of proteins, a histidine kinase and a response regulator. Upon receiving an input signal, a histidine kinase autophosphorylates and then catalyzes transfer of its phosphoryl group to a cognate response regulator, which often activates a transcriptional response. …
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Molecular and Biochemical Analysis of the Histidine Kinase CusS and its Role in Metal Resistance in Escherichia coli
… reactions between the prototypical histidine kinase CusS and the response regulator CusR. Copper sensing by the periplasmic domain of CusS is proposed to initiate signal transduction in the Cus system. Despite the frequency with which bacteria employ histidine kinases to sense their …
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Analysis of signal output by the ethylene receptor ETR1 from Arabidopsis
… of five members: ETR1 and ERS1 have a functional histidine kinase domain and form subfamily 1; members of subfamily 2, including ETR2, ERS2, and EIN4, possess a highly diverged histidine kinase domain predicted not to be functional. To analyze signal output by the ethylene receptor ETR1 from …
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A novel two-component signal transduction system in propionibacterium acnes and its association with a putative extracellular signalling peptide
… signalling peptide, and divergently transcribed histidine kinase and response regulator. The aim of this project is to investigate the relationship among these three components as being elements of a putative quorum sensing system. Using purified proteins and in vitro phosphorylation assays, the …
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Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in V. alginolyticus
… and ATP binding (CA) domain, a dimerization and histidine phosphor-transfer (dHp) domain and a response regulator. For REC domain, itasser homology model and limited proteolysis revealed a two-domain structure connected by a flexible linker. Based on B-factor of linker truncation may be necessary …
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Structural Studies on the Regulation of Sporulation in Bacillus and Antibiotic Sulfation
… of a Geobacillus stearothermophilus sporulation histidine kinase in complex with an inhibitor of the kinase reaction (Chapter 2). (2) Developed an approach using the methods of protein semisynthesis and small molecule organic chemistry that will facilitate the study of active state conformations …
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Pervasive degeneracy and epistasis in a protein-protein interface
… These pathways, typically consisting of a histidine kinase and a response regulator, are an ideal model system for studying protein-protein interactions as they rely almost exclusively on molecular recognition for specificity. The kinase uses a limited set of residues to recognize the …
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Mechanism and specificity of bacterial two-component signaling systems
… of paralogous proteins; often hundreds of the histidine kinase (HK) and response regulator (RR) pairs that make up a TCS system can be found in a single cell. How do these systems maintain faithful signal transmission and avoid cross-talk? To understand how specificity is determined, we …
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Photosensing with LOV: Molecular Insights into the Shared Mechanism of Light-Regulated Kinase Signaling between Plant Phototropins and Bacterial Two-Component Systems
… on characterizing the role of LOV domains in kinase signaling and the mechanism of signal propagation from LOV domains to a diverse set of downstream effectors. I started from the studies of the LOV domain regulated serine/threonine kinase activity from plant phototropins, later comparing this …
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Role of the Nla6S and Nla28S histidine kinases in fruiting body development of <i>Myxococcus xanthus</i>
… changes in gene expression. They contain a histidine kinase sensor that detects environmental cues and a response regulator that modulates cellular processes. Two key regulators of the early stages of fruiting body development are the Nla6S/Nla6 and Nla28S/Nla28 TCSs. The response regulators …
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Threonine Phosphorylation Regulates Two-Component Systems Involved in Cell Wall Metabolism
… systems (TCS). TCS are composed of a sensor histidine kinase that upon activation autophosphorylates a conserved histidine residue and then transfers this phosphate to a response regulator at a conserved aspartate residue. This phosphorylation event leads to dimerization of the response …
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Induction kinetics of the PhoQ-PhoP two-component system in Escherichia coli
… factors. Upon activation, the bifunctional histidine kinase PhoQ autophosphorylates and subsequently phosphotransfers to the response regulator PhoP, thereby activating it to increase transcription of PhoP target genes. Because PhoQ is bifunctional, PhoQ acts as a phosphatase on …
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Structural Basis of Bacterial Transmembrane Signaling
… McpZ in Sinorhizobium meliloti and the signaling histidine kinase GacS in Pseudomonas aeruginosa. What made this work particularly challenging is that the ligands that induce signal transduction across the inner cell membrane in either protein remain unidentified. We solved the crystal structure …
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Investigation of two-component signaling systems in Pseudomonas aeruginosa and their roles in the mucus barrier
… are typically comprised of a membrane-bound histidine kinase that senses external cues signals, and a cognate cytoplasmic response regulator that triggers changes in gene expression. The importance of these systems is highlighted by the fact that they have been identified in the vast majority …
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Towards unveiling the Photoactive Yellow Proteins: characterization of a halophilic member and a proteomic approach to study light responses
… (Bph) domain and by a C-terminal histidine kinase domain. The PYP domain acts as a blue-light switch reversing the effects of red light on the Bph domain, resulting in a light-regulated histidine kinase activity of Ppr. Comparative proteomics of wild-type Rh. centenum relative to a …
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Investigation of the Roles of CheD in the Bacillus Subtilis Chemotactic Signal Transduction
… chemoreceptors, and then transmitted to the histidine kinase CheA. Once activated, CheA interacts with the response regulator CheY through phosphorelay, which causes a change in the rotation of the flagella. The direction of flagella rotation determines whether a cell swims straight or just …
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Studying the Competence Regulon Quorum Sensing Circuity in Streptococcus mitis
… and binding of CSP and its analogs with the histidine kinase receptor, ComD. We isolated the CSP and confirmed its sequence by MS/MS analysis. Analyzing the structure-activity relationship of CSP's alanine screen analogs gave us insights of their binding and interaction with ComD receptor. …
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Genetic Control of Root System Architecture in Rice
… a mutation in a gene encoding a putative rice histidine kinase (OsHK1) that results in plants with increased seedling root depth. Using time-lapse imaging, we show that OsHK1 mutants have reduced circumnutation, or circular root tip growth. This supports a previously underappreciated link …
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Structural Studies of the Bacterial Histidine Kinases RetS and GacS, Key Components of the Multikinase Network that Controls the Switch Between a Motile Invasive Lifestyle and a Sessile Biofilm Lifestyle in Pseudomonas aeruginosa
… as their primary means of signal transduction. Histidine kinase (HK) and response regulator (RR) proteins comprise these TCSs and phosphorelays. Previously, signal transduction within TCSs and phosphorelays was thought to only occur through a linear series of phosphotransfers between HKs and …
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