Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"His-tagged"”.
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EFFECT OF HISTIDINE TAG ON THE ENZYMATIC ACTIVITY OF PSEUDOLYSIN AND ITS INTERACTION WITH THE STREPTOMYCES METALLOPROTEASE INHIBITOR (SMPI) PROTEIN
… caused by <em>P</em>. <em>aeruginosa</em>. This study investigated the effect of N-terminal Histidine (His) tag on the enzymatic activity of PLN and its interaction with SMPI. His-tagged PLN and SMPI proteins were expressed in bacteria and purified by affinity chromatography and gel …
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The development of a model system and high throughput assay for the study of interactions between zinc finger proteins and DNA
… finger protein was initially synthesised as a His tagged product. It was not possible however to develop a high throughput assay using the His tagged zinc finger protein. The gene encoding the zinc finger protein was altered and the protein synthesised as a Glutathione S-Transferase (GST) …
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Studies of Lipoic Acid Related Enzymes
… chain fatty acid-CoA ligases. I expressed the His-tagged protein at a high level in E. coli and purified it to homogeneity. To explore the physiological application of this enzyme in E. coli fatty acid metabolism, I constructed the first E. coli fabH (beta-ketoacyl-ACP synthetase III) bypass …
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Synthesis and performance of novel supramolecular tools for single-particle cryogenic electron microscopy and drug and gene delivery
… lipid coatings were then used to capture His6-T7 bacteriophage and His6-RplL from cell lysates, purified His8-GFPuv, and nanodisc embedded His6-MalFGK2. Graphene-oxide-NTA-based affinity grids were fabricated via covalent modification of graphene-oxide and monolayer fabrication on a …
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Homology Cloning, Heterologous Expression and Characterization of A New Channelrhodopsin
… function as light-gated cation channels and this channel activity has been exploited to trigger action potentials in neurons with light to control neural circuits (“optogenetics"). Four channelrhodopsins were identified in two algal species, <em>Chlamydomonas reinhardtii</em> and <em>Volvox …
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FIV-FCA and FIV-PCO Reverse Transcriptases: Expression, Purification and Activity Comparison
… enzymes from a virus with a long coevolutionary history with its host will have higher processivity. In this thesis we expressed, purified and compared the processivity of recombinant His-tagged RTs of FIV-Fca and FIV-Pco. Both of the RTs contained a hexa N-terminal His tag and were expressed in …
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FIV-FCA and FIV-PCO Reverse Transcriptases: Expression, Purification and Activity Comparison
… enzymes from a virus with a long coevolutionary history with its host will have higher processivity. In this thesis we expressed, purified and compared the processivity of recombinant His-tagged RTs of FIV-Fca and FIV-Pco. Both of the RTs contained a hexa N-terminal His tag and were expressed in …
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Functional studies on the extrinsic proteins of Photosystem II
… were produced, which contained an N-terminally His6-tagged PsbO-1 protein. Photosystem II closure kinetics demonstrated that the defective double reduction of QB and the delayed exchange of QBH2 with the plastoquinone pool in the psbo1 mutant were effectively restored to the wild type levels by …
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Recombinant Expression In Pichia pastoris System Of The Potassium Channel Inhibitor Peptide, Ts6.
… of being treated have not been discussed. In this study, Ts6 which has proven blocking activity in Kv1.3 has been analyzed recombinantly for Kv1.5 and Kv1.3. This toxin (Ts6) purified from Tityus serrulatus venom (a Brazilian scorpion) is composed of 40 amino acid residues, four disulfide …
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Design of Metalloporphyrin Liposome for Biomedical Applications
… unique chemical property of metalloporphyrin, this thesis focuses on exploring the novel biomedical applications of metalloporphyrin liposome. Chapter 1 provides a comprehensive background on metalloporphyrin, especially reviewing existing metalloporphyrin nanoparticles for theranostic …
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The role of latent membrane protein 2 in the pathogenesis of Epstein-Barr virus associated malignancy
… pathogenesis of HL remains largely unknown. In this study, HL cell lines stably expressing LMP2A and LMP2B HL were generated. The impact of global gene expression in KMH2 cells expressing LMP2A and LMP2B was performed using Gene expression microarrays; this identified a number of potentially …
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Biochemical Evaluation of SRH Analogs as Potential LuxS Inhibitors
… interspecies quorum sensing. The goal of this project is to establish biochemical assays for the evaluation of small molecules as potential LuxS inhibitors. The first assay is a conventional colorimetric assay that utilizes Ellman’s reagent to quantify the homocysteine byproduct of DPD …
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Identification of surface protein complexes of Streptococcus pyogenes through protein microarray technology
… under reasonably straightforward conditions. In this study approximately 100 proteins of Group A Streptococcus (GAS) predicted to be secreted or surface exposed by genomic and proteomic approaches were purified in a His-tagged form and used to generate protein microarrays on nitrocellulose-coated …
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Haem and xenosiderophore mediated iron acquisition by sinorhizobium meliloti and pseudomonas aeruginosa
… and purification of full-length recombinant His-tagged FoxB was achieved.
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Regulation der Transkription des hspA-Gens in Stigmatella aurantiaca und Analyse der Funktion des HspA-Proteins
… core RNA polymerase binding sites. Deletion of this region completely blocks hspA transcription. The second domain carries putative regulator-binding sites. Heat shock and phosphorylation enhance the binding of the regulator(s) to the hspA promoter. Deletion of this region reduces hspA …
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Molecular genetics and microbiology of bioremediation using methane-oxidising bacteria
… steps, a new system was developed by inserting a His-tag in the wild type hydroxylase β-subunit. The His-tag hydroxylase was then purified in one step by using an affinity column. The new His-tag system yields hydroxylase with detectable activity when it was tested toward propylene. Solid phase …
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Modulation of Immunity via Antibodies Displayed on in situ Forming Peptide Co-assemblies
… can lead to severe immune related side effects. This dissertation investigated a versatile platform to display antibodies locally in diseased tissues to minimize unwanted systemic exposure. This system is a stimuli-responsive and injectable formulation utilizing in situ forming peptide …
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Mapping protein-protein interactions in the <i>Escherichia coli</i> Twin Arginine Translocase
… experiments in detergent solution, using a His-tagged TatC variant, resulted in co-purification of TatB along with a small amount of associated TatA. The amount of co-purifying TatA was notably increased when TatB was absent, suggesting that the proteins may compete for the same binding site …
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Untersuchung und Spezifizierung der molekularen Interaktion von Ubiquitin und Sumo mit dem rekombinanten a1-Glyzinrezeptor
… and ubiquitination of membrane proteins – in this case of the GlyR alpha 1-subunit and its ubiquitin mediated internalisation – is described. The clinical relevance of those mechanisms is wide spread and would in this case produce an inadequate receptor regulation with the expected …
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Molecular Characterization of Mth203 protein
… with MthCdc6-1 in a yeast two-hybrid screen and His-tagged full-length protein pull-downs. Mth203 is a SF2 family helicase. The proteins of this superfamily perform a wide array of functions in DNA/RNA processing and often are a part of multi-protein complexes. The Mth203 homologue (Mmp0457) in …
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