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Showing 1 to 2 of 2 for “"His-tag purification"”.
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Synthesis and Applications of Error Free DNA Using Polymerase-Enabled Rapid, Frugal Error Correcting Technology (PERFECT) PCR
… Escherichia coli BL21(DE3) and purified through his-tag purification. We analyzed the enzyme's activity, kinetics, thermal stability, and optimal concentrations for DNA template error corrections. The second aim focused on integrating the NucS enzyme into PCR to establish a Polymerase-Enabled …
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Single copy gene expression system as a tool for the purification of membrane proteins from Pseudomonas aeruginosa
… clinical use. One of the major contributors to this resistance is the active efflux of antibiotics from the cell, primarily by action of the Resistance Nodulation Division (RND) family of efflux pumps. These pumps are composed of three proteins; an inner membrane RND pump, a periplasmic membrane …