Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"His Tag"”.
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Production and Characterization of Monoclonal Antibodies against a Recombinant Nfa1 Protein Cloned from Pathogenic Naegleria fowleri
… monoclonal antibodies against a recombinant His-tag fusion Nfa1 protein cloned from pathogenic N. fowleri were produced, and its characterization was elucidated. In addition, to observe the usage in immunological studies, the localization of the Nfa1 protein in N. fowleri was observed by …
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Ortsgerichtete Immobilisierung fluoreszenzmarkierter IL-6-Typ-Zytokine und Analyse ihrer Signaltransduktion
… were fused at the N-terminus with different tags by use of molecular genetics methods. After expression of these recombinant cytokines in E. coli or HEK293-cells, these tags enabled a) the purification (His-tag), b) a reproducible, site-specific coupling in bioactive orientation (His-tag or …
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EFFECT OF HISTIDINE TAG ON THE ENZYMATIC ACTIVITY OF PSEUDOLYSIN AND ITS INTERACTION WITH THE STREPTOMYCES METALLOPROTEASE INHIBITOR (SMPI) PROTEIN
… caused by <em>P</em>. <em>aeruginosa</em>. This study investigated the effect of N-terminal Histidine (His) tag on the enzymatic activity of PLN and its interaction with SMPI. His-tagged PLN and SMPI proteins were expressed in bacteria and purified by affinity chromatography and gel …
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Molecular genetics and microbiology of bioremediation using methane-oxidising bacteria
… steps, a new system was developed by inserting a His-tag in the wild type hydroxylase β-subunit. The His-tag hydroxylase was then purified in one step by using an affinity column. The new His-tag system yields hydroxylase with detectable activity when it was tested toward propylene. Solid phase …
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Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT)
… from Plasmodium falciparum from a vector with a His-tag facility to one without, expression of the protein in E. coli, and purification. On an analytical scale (40 ml culture), a purification procedure was developed that involves extraction of contaminating proteins by anion exchange …
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Cloning and Expression of Bovine Alpha S1 Casein Peptides of Variable Size and Position of spPEP Inhibitor Sequence
… to hPEP (Jeanneret, 2011). The Objective of this study was to produce 16 recombinant peptides derived from αS1casein of varying lengths and varying placement of the non-allergenic inhibitory sequence. The inhibitory region is positioned at 0, 10, 20 and 30 amino acids from either N’ terminal …
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MOLECULAR CHARACTERIZATION AND EXPRESSION OF GENTISATE 1,2-DIOXYGENASE FROM PSEUDOMONAS ALCALIGENES NCIB 9867
… generated by both random and site directed mutagenesis of the gene cloned from Pseudomonas alcaligenes NCIB 9867. Alignment of known GDO sequences indicated the presence of a conserved central core region containing a two-motif signature of the cupin superfamily, designated motifs 1 and 2. …
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Modulation of Immunity via Antibodies Displayed on in situ Forming Peptide Co-assemblies
… can lead to severe immune related side effects. This dissertation investigated a versatile platform to display antibodies locally in diseased tissues to minimize unwanted systemic exposure. This system is a stimuli-responsive and injectable formulation utilizing in situ forming peptide …
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FIV-FCA and FIV-PCO Reverse Transcriptases: Expression, Purification and Activity Comparison
… enzymes from a virus with a long coevolutionary history with its host will have higher processivity. In this thesis we expressed, purified and compared the processivity of recombinant His-tagged RTs of FIV-Fca and FIV-Pco. Both of the RTs contained a hexa N-terminal His tag and were expressed in …
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FIV-FCA and FIV-PCO Reverse Transcriptases: Expression, Purification and Activity Comparison
… enzymes from a virus with a long coevolutionary history with its host will have higher processivity. In this thesis we expressed, purified and compared the processivity of recombinant His-tagged RTs of FIV-Fca and FIV-Pco. Both of the RTs contained a hexa N-terminal His tag and were expressed in …
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Recombinant Expression In Pichia pastoris System Of The Potassium Channel Inhibitor Peptide, Ts6.
… of being treated have not been discussed. In this study, Ts6 which has proven blocking activity in Kv1.3 has been analyzed recombinantly for Kv1.5 and Kv1.3. This toxin (Ts6) purified from Tityus serrulatus venom (a Brazilian scorpion) is composed of 40 amino acid residues, four disulfide …
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NON-FOULING AFFINITY PLATFORMS FOR PROTEIN IMMOBILIZATION IN ELECTRON MICROSCOPY
… system. The selective and reversible capture of his-tag T7 bacteriophage, RplL, and GroEL from crude lysates, as well as purified nanodisc-solubilized his-malFGK2, on these NTA-modified grids with an exceptionally low level of adsorption by non-target proteins has been observed. Our data …
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Herstellung von Biochips basierend auf selbstaggregierender Monolagen
… Analyse zu ermöglichen. Die Verwendung von Selbstaggregierenden Monolagen (SAMs) ist für die oben genannten Ziele eine ideale Plattform. Durch diese Monolagen lassen sich chemische und physikalische Eigenschaften der Oberflächen sehr genau kontrollieren. Sie dienen als Bindeglied zwischen …
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Pretargeted cancer radioimmunotherapy and radioimaging using antibodies and antibody fragments
… antibodies to achieve maximum tumor binding. This slow blood clearance results in high normal tissue irradiation and a poor therapeutic index. This is exemplified by the fact that to date, only two radiolabeled antibodies have been approved by the FDA for radioimmunotherapy of cancer. … …
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A novel approach for the suppression of photorespiration in C 3 plants by gene transfer
… vectors in N-terminal translational fusion to a His-tag. The genes were first expressed in bacteria and the enzymatic activity of the constructs was shown. Transgenic tobacco plants were created containing all genes necessary for the proposed pathway by plastdial as well as nuclear …
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Development of Building Blocks - Thermostable Enzymes for Synthetic Pathway Biotransformation (SyPaB)
… of enzymes and cofactors. To overcome this obstacle, strong motivations have driven intensive efforts in discovering, engineering, and producing thermostable enzymes. In this project, ribose-5-phosphate isomerase (RpiB), one of the most important enzymes in the pentose phosphate pathway, …
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Ric-8: un nuevo GEF con estructura tipo "armadillo"
… about its structure. Therefore, the aim of this thesis was to study the relationship between structure and function on RIC-8, using as model RIC-8 from X. laevis. Analysis in its primary structure did not give us information about the function of xRIC-8 and RIC-8 proteins were showed as a …
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Characterization of XVEF and XvCaM : two calcium-binding proteins isolated from the resurrection plant Xerophyta viscosa
… in E. coli as a fusion protein with a 6X His-tag and it was shown to be a functional protein that binds Ca2+ by utilizing a 45CaCh overlay assay. Northern blot analyses of XvCaM using total RNA under low (4°C) and high temperature treatment (42°C) showed constitutive expression levels of …
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The effect of bacterial vaginosis on HIV infection
… play a role in enhancing HIV acquisition. This study investigated whether VLY, sialidase and biofilm formation played a role in HIV infection. Recently, it was discovered that Gardnerella spp. comprises G. leopoldii, G. vaginalis, G. piotii and G. swidsinskii as well as nine other genome …
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Enhancement of plant expression vectors using replication and silencing suppressor elements
… for difficult to express proteins. In this study virus-derived silencing suppressors and replication elements were used with the aim of increasing expression and yield of enhanced green fluorescent protein (EGFP) and the Zika PrME polyprotein in Nicotiana benthamiana. A comparison of …
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