Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"High-throughput assays"”.
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Integrative Biomarker Identification and Classification Using High Throughput Assays
… all aspects of cell activity. Recent advances in high throughput technologies allow biologists to generate massive amounts of data, more than might have been imagined decades ago. These advances have made it possible to launch comprehensive projects such as (TCGA) and (ICGC) which systematically …
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A Critical Evaluation of Screening versus Sample-by-Sample Analysis of Protein Aggregation using a Variety of Chromatographic and Spectroscopic Techniques
… variety of chemical and biophysical techniques. High-throughput assays are commonly utilised for screening protein formulations. Two high-throughput assays were critically examined to assess the effectiveness and robustness of this approach for detecting protein aggregation after forced …
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Analysis of DNA-binding Proteins in Yeast Saccharomyces Cerevisiae
… in vivo remains a bottleneck. I developed two high-throughput assays to address this issue. The first is a modification of the yeast "one-hybrid" assay. The second is probing protein microarrays with DNA sequence elements. Using these methods, I identified two proteins, Sef1 and Yjl103c, that …
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Mechanistic Studies and Inhibition of N-hydroxylating Monooxygenases
… containing siderophores. Siderophores are high affinity iron-chelators composed of catechol and hydroxamate functional groups that are synthesized and secreted by several microorganisms and plants. It has been showed that many NMOs are essential for virulence in many opportunistic pathogens …
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Inferring hypotheses from complex profile data - by means of CSB.DB, a comprehensive systems-biology database
… data triggered the development of multiplex high-throughput assays allowing simultaneous measurement of transcripts, proteins and metabolites. With genome information and profiling technologies now in hand a highly parallel experimental biology is offering opportunities to explore and …
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The microbiology of diabetic foot infections: a Ghanaian perspective
… hospital admissions (53%) among diabetics with high rates of amputation (33.3%) and death (8.8%). Diabetic foot ulcers are predisposed to infections from bacteria in the environment which normally colonise these wounds as multicellular communities called biofilms. Biofilms have been found to …
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Evaluating Chemical Toxicity: A Novel Behavioral Assay Using Daphnia
… of their toxicity. There is a need for high-throughput assays to rapidly assess the toxicity of these emerging contaminants. A behavioral assay utilizing freely swimming Daphnia pulex was developed to evaluate the sub-lethal chemical effects. Daphnia, a keystone species, are small …
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Characterization of the Non-Proteolytic Mechanism and Cellular Site of Action of PCSK9-Mediated Degradation of the Low-Density Lipoprotein Receptor
… class (Class VI) of FH mutants. Finally, two high-throughput assays were developed to discover new small molecule inhibitors of intracellular PCSK9 autocleavage and to identify previously unrecognized protein activators of PCSK9 action. Use of these assays could provide additional avenues for …
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Engineering synthetic biology routes for protein analysis using microfluidic systems
… to be relished and divided up for additional assays. Furthermore, the system allows for parallelisation, this way many identical reactions or assays can be replicated on a single microfluidic chip hence increasing throughput, a quality very much desired by pharmaceutical companies.Different …
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Enhancing the Industrial Potential of Filamentous Cyanobacteria
… SYTO® 9 is a reliable assay and can be used in high-throughput assays. To develop cyanobacteria strains with increased tolerance to biofuels, directed evolution under the pressure of higher biofuel concentrations was used. As these biofuels are highly volatile, it was necessary to conduct …
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Targeting survival pathways in cancer stem cells
… failed to generate distant lesions. Afterwards, high-throughput assays have been exploited in order to define molecular mechanisms underlying this differential metastatic proclivity. In particular, most up- and down-regulated genes were evaluated for their convergence on canonical signal …
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Identification and characterisation of host-pathogen protein-protein interactions in the blood stages of malaria
… in our understanding of parasite biology and in high-throughput biomolecular interaction detection methods have paved the way to a number of successes. In this work I produced a library of recombinant Plasmodium falciparum proteins to screen for interactions with human receptors in a number of …
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Leveraging genomic and molecular variations to understand the regulatory landscape in human cancers and differentiating stem cells
… As technological advances have resulted in high-throughput assays for different molecular dimensions, it has become challenging to turn these large-scale data into meaningful insights and to delineate biological cause and consequence. In this thesis, I use computational modelling to detect …
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Engineering TEV Protease Specificity: An Exploration of Machine Learning and High-Throughput Experimentation for Protein Design
… to engineer TEV (tobacco etch virus) protease, a highly sequence-specific protease, to cleave alternative substrates. We incorporate novel high-throughput assays and powerful machine learning (ML) methods for highly effective protein engineering. The first portion of this thesis focuses on …
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An investigation of the mechanisms of piperaquine resistance in Plasmodium falciparum malaria
… resistance. The study first developed high-throughput assays to reliably detect one of these markers, a copy number variation (CNV) in the plasmepsin 2 and plasmepsin 3 (PM2-PM3) genes, in parasites likely to be PPQ-resistant. In addition to assay development, this study used gene …
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A genetic analysis of molecular traits in skeletal muscle
… traits, combined with the development of high throughput assays, has motivated the mapping of molecular quantitative trait loci (QTLs), genetic associations with molecular traits such as gene expression (eQTLs) and DNA methylation (mQTLs). The Finland-United States Investigation of NIDDM …
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Network to pology In Human Protein Interaction Data Predicts Functional Association
High-throughput assays, such as yeast two-hybrid system, have generated a huge amount of protein-protein interaction (PPI) data in the past decade. This tremendously increases the need for developing reliable methods to systematically and automatically suggest protein functions and relationships …
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Next generation approaches toward engineering therapeutic proteases
… predicated on the availability of quantitative high-throughput assays that maintain the essential link between genotype and phenotype. In this work we have investigated a variety of novel single cell fluorescence assays and selections for engineering protease substrate specificity and …
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Analysis and Redesign of Protein-Protein Interactions: A Hotspot-Centric View
… For example, small interfaces can have high affinity, and when multiple binding partners are generated to the same protein, they are predisposed to binding the same regions and often have the same hotspots. Even small molecules that bind to interfaces and disrupt protein-protein …
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