Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"High-throughput DNA sequencing"”.
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Expanding the applications of high-throughput DNA sequencing
DNA sequencing is the process of determining the identities of the nucleotides that make up a molecule of DNA. The rapid pace of advancements in sequencing technologies in recent years have made it possible to simultaneously determine the sequences of hundreds of millions of short DNA fragments. …
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Elucidating aquatic virioplankton diversity and dynamics using high-throughput DNA sequencing
… in viral ecology was the application of high-throughput DNA sequencing to entire viral communities. Known as shotgun viral metagenomics, this approach allows access to the majority of viruses that cannot be maintained in culture. Viral metagenomics has revealed surprising insight into …
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Molecular display of synthetic oligonucleotide libraries and their analysis with high throughput DNA sequencing
High throughput methods in molecular biology have changed the landscape of biomedical research. In particular, advances in massively parallel DNA sequencing and synthesis technologies are defining our genomes and the products they encode. In the first part of this thesis, we have constructed a …
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Computational methods for high-throughput pooled genetic experiments
Advances in high-throughput DNA sequencing have created new avenues of attack for classical genetics problems. This thesis develops and applies principled methods for analyzing DNA sequencing data from multiple pools of individual genomes. Theoretical expectations under several genetic models are …
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High-throughput methods for characterizing the immune repertoire
… have been limited by the use of traditional, low-throughput technologies. In this thesis, we leverage recent advances in high-throughput DNA sequencing technology to develop new methods to characterize and probe the immune repertoire in unprecedented detail. We use this technology to 1) …
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Indoor biological exposures : what can HVAC filter dust tell us?
… the home is crucial. Recently, rapid advances in high-throughput DNA sequencing technology have spurred increased study of the relationships between the human and built environment microbiomes. HVAC filters hold promise as long-term, spatially integrated, high volume samplers to characterize the …
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Ehrlichia chaffeensis TRP47 enters the nucleus via a MYND-binding domain-dependent mechanism and predominantly binds enhancers of host genes associated with signal transduction, cytoskeletal organization, and immune response
… demonstrated that TRP47 directly binds host DNA via its tandem repeat domain. Utilizing chromatin immunoprecipitation followed by high-throughput DNA sequencing (ChIP-seq) with E. chaffeensis-infected cells, TRP47 was found to bind at multiple sites in the human genome (n = 2,051 at p < …
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The impact of whey protein consumption and exercise on the composition and diversity of the gut microbiota: a high through-put DNA sequencing approach
… technologies over the last decade have highlighted the pivotal role which the gut microbiota plays in maintaining human health. Conversely, perturbations to the composition or actions of the ‘normal/functioning’ microbiota have been frequently associated with the pathogenesis of several …
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Deep Learning Based Proteomic Language Modelling for in-silico Protein Generation
… of genomic data that is now available due to high-throughput DNA sequencing, it is now possible to examine protein families in a scale and resolution that has never before been possible. By using this abundance of data to learn high dimensional representations of amino acids sequences, in this …
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Systems level investigation of the genetic basis of bovine muscle growth and development
… compensatory growth (chapter 5). Finally, using high-throughput DNA sequencing, a candidate list of 200 genes was interrogated to identify genes which may be evolving at different rates, and under evolutionary selection pressure, in beef compared to dairy animals (chapter 6). A number of genes …
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Antibiotic resistance in the gut microbiota
… infants compared to untreated controls using high-throughput DNA sequencing. The results demonstrated the significant effects of antibiotic treatment, resulting in increased proportions of Proteobacteria and decreased proportions of Bifidobacterium. The species diversity of bifidobacteria was …
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Characterization of novel prophages in lysogenic murine isolate lactobacillus murinus EF-1
… sample. After initial antibiotic induction, high-throughput DNA sequencing and assembly identified the potential lysogenic bacterium as L. murinus EF-1. A 2.30 Mbp draft genome was generated with a 39.6% G+C content and 2196 identified coding regions. These metrics are similar to other …
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Biogeochemical investigation of centrifuged fine tailings deposits at an oil sands mine in Northern Alberta, Canada
… in oil sands process-affected water (OSPW). High sodium (Na) concentrations in OSPW increase the electrical double layer (EDL) thickness at clay-mineral surfaces, which hinders aggregation and, therefore, settlement. Production of CFT involves dredging FFT from tailings ponds, amending with …
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Computational analysis of eukaryotic metatranscriptomes from diverse marine environments
… in the Earth's atmosphere. Recent advances in high-throughput DNA sequencing technologies have allowed scientists to sample the set of genes actively transcribed from communities of microbes in-situ. This set of transcripts (the metatranscriptome) provides a snapshot of actively transcribed …
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Assessment of stress-induced and developmentally-induced DNA methylation changes in barley (Hordeum vulgare L.)
DNA methylation is involved in both plant development and adaptation to environmental stress. Changes in DNA methylation can affect the expression of genes that are important for both plant tissue differentiation and stress response. Characterisation of tissue and stress specific methylation …
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Leveraging community-based sequencing approaches to characterise the dairy microbiome
… specific groups of microorganisms, advances in high-throughput DNA sequencing have enabled the study of entire microbial communities in food or food-related samples. In this thesis, shotgun metagenomic sequencing was employed to investigate microbial communities in bulk tank milk. First, shotgun …
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Malleefowl and anthropogenic change: an integrated analysis of population trends, landscape genetics and movement ecology
… leading to more frequent and longer periods of high temperatures, reduced precipitation and increased frequency and intensity of wildfires. This thesis aimed to investigate how historic and recent habitat fragmentation and climatic variables influenced Malleefowl on the Eyre Peninsula and how …
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Developing high-resolution methods to study DNA and RNA dynamics
Nucleic acids, including DNA and RNA, are one of the most important biomacromolecules inside the cell. DNA stores genetic information, while RNA has more versatile roles including conveying and deciphering genetic information, catalyzing biological reactions, conducting post- transcriptional …
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The Last Stand of Angraecum: Biogeographic and Evolutionary History of Darwin's Muses
… a limited number of markers derived from Sanger sequencing have proved to be insufficient for informing the construction of such a classification. Using Angiosperms353, a target sequence capture probe set, hundreds of low-copy nuclear genes were sequenced from a broad sampling of …
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Species response to shifting and variable climates
… (n = 3 taxa) from this vast region. Using high-throughput DNA sequencing, hindcast Species Distribution Modeling, and a reassessment of pollen records, I found evidence that Alnus expanded from several population nuclei (i.e. refugia) that existed during the LGM and coalesced during the …
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