Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"High throughput technologies"”.
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Novel High-throughput Technologies for Applications in Microbiology
… protocol is laborious, time-consuming, low throughput, and operator dependent, hindering the progress of bacterial genetic engineering. To address these challenges, this study focuses on developing high-throughput solutions to streamline the process, improve efficiency, and ensure consistent …
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Exploring protein fitness landscapes with new high-throughput technologies
… climb through a fitness landscape, reaching higher fitness (or activity) with each step (or mutation). The valleys of such a space relate to the starting points of protein engineering campaigns. Understanding this area could enlighten principles of how proteins quickly adapt in nature and …
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Novel Therapeutic Targets Identified By High-Throughput Technologies For Triple-Negative Breast Cancer
… cancers.</p> <p>681 genes showed consistent and highly significant overexpression in TNBC compared to receptor-positive cancers in 2 data sets. For two genes, 3 of the 4 siRNAs showed preferential growth inhibition in TNBC cells. These two genes were the low density lipoprotein receptor-related …
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Integrating High-throughput Technologies for the Identification and Validation of Novel Ovarian Cancer Biomarkers
… in North America. Although survival rates are high when the disease is diagnosed at an early stage, this decreases significantly in late-stage diagnoses. The majority of cases are of epithelial origin, which can be classified into four major subtypes: serous, mucinous, endometrioid and clear …
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Integration of Genome Scale Data for Identifying New Biomarkers in Colon Cancer: Integrated Analysis of Transcriptomics and Epigenomics Data from High Throughput Technologies in Order to Identifying New Biomarkers Genes for Personalised Targeted Therapies for Patients Suffering from Colon Cancer
Colorectal cancer is the third most common cancer and the leading cause of cancer deaths in Western industrialised countries. Despite recent advances in the screening, diagnosis, and treatment of colorectal cancer, an estimated 608,000 people die every year due to colon cancer. Our current …
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Enhancing Workflows in Biologics Drug Substance Process Development Through Automation
… development is through the implementation of high throughput technologies, equipment (and associated methods and software) used to generate and process large amounts of data in little time. It is important, however, that implementation of these solutions is optimized across the entire process …
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Extensible neural network software : applications in gene expression analysis
… life sciences for analysis of large data sets. High-throughput technologies, such as gene expression microarrays, have challenged traditional statistical learning algorithms given their high dimensionality. This thesis describes GAINN, a neural network software package I created. GAINN was …
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Navigating exponentially large spaces in biology : methods for directed evolution and smFRET time series analysis
The recent explosion of high throughput technologies in many fields of biology has necessitated the use of sophisticated algorithms to guide experimental design and analyze results. This thesis explores two such fields: directed protein evolution and single molecule fluorescence resonance energy …
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Artificial neural network techniques to investigate potential interactions between biomarkers
High-throughput technologies in biomedical sciences, including gene microarrays, supposed to revolutionise the post-genomic era, have barely met the great expectations they inspired to the biomedical community at first. Current efforts are still focused toward improving the technology, its …
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Attributed Graph Classification via Deep Graph Convolutional Neural Networks
… deep neural network model which captures the higher-order latent attributes of weighted, labeled, undirected, attributed graphs of arbitrary size. The architecture of AGCP is an efficient variant of convolutional neural network (CNN) and has a linear filter function that convolves over the …
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Systems biology of breast cancer
… is the focus of this thesis. The development of high throughput technologies such as expression microarrays has paved the way for investigating thousands of genes at a time. Microarrays with their high data volume, multivariate nature and non-linearity pose challenges for analysing using …
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Harnessing Cancer Omics to Inform Precision Oncology
<p>The development of high-throughput technologies greatly facilities precision oncology in the era of big data. With the growing size of pan-cancer genomic, transcriptomic and proteomic profiling data, there is imperative need for integrative analysis of molecular and clinical information in an …
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Proteomic analysis of biomarkers associated with immunotherapy in murine tumour models
Emergence of proteomics and high-throughput technologies has allowed the identification of protein expression patterns of disease that potentially hold clinical importance in predictive medicine. The analysis of complex data generated by these technologies incorporates the use of computer …
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Refinement of Subnetwork Discovery Algorithm for Biological Networks
… of biological experimental data from new high-throughput technologies, such as microarray data, suggests the need for new methods to study biological processes using a systems-based approach. Handling this huge volume of data requires the development of new computational methods to analyze …
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Detecting Phenotype-Specific Interactions Between Biological Processes From Microarray Data And Annotations
<p>The development of high throughput technologies such as DNA microarrays has enabled researchers to measure expression levels on a genomic scale. Correct and efficient biological interpretation of the voluminous data generated by these technologies, however, remains a challenging problem. A …
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Development and Validation of Quantitative PCR Assays for DNA-Based Newborn Screening of 22q11.2 Deletion Syndrome, Spinal Muscular Atrophy, Severe Combined Immunodeficiency and Congenital Cytomegalovirus Infection
The development of new high throughput technologies able to multiplex disease biomarkers as well as advances in medical treatments has lead to the recent expansion of the newborn screening panel to include DNA-based targets. Four rare disorders; deletion 22q11.2 syndrome and Spinal Muscular Atrophy …
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High-Throughput Methods For Reaction Development Using The Mosquito® Liquid Handling Robot
High-throughput technologies have dramatically advanced processes such as drug screening and protein crystallisation. Application of related high-throughput experimentation practices have the potential to revolutionise chemical synthesis. This thesis describes the standardisation and implementation …
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Analysis of fecal biomarkers to impact clinical care and public health
DNA sequencing and metabolomics technologies have accelerated the discovery of novel biomarkers in clinical samples. In this thesis, I explore the potential of fecal biomarkers to impact clinical and public health practice through non-invasive assessments. First, I highlight the potential of the …
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Bayesian Modeling of Complex High-Dimensional Data
With the rapid development of modern high-throughput technologies, scientists can now collect high-dimensional complex data in different forms, such as medical images, genomics measurements. However, acquisition of more data does not automatically lead to better knowledge discovery. One needs …
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Comprehensive analysis of high-throughput experiments for investigating transcription and transcriptional regulation
… to answer these questions frequently make use of high-throughput technologies that allow for investigation of multiple genomic regions, or transcribed copies of genomic regions, in parallel. In this dissertation, I present three high-throughput studies I have been involved in, in which data gained …
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