Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"High throughput imaging"”.
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OrganixInsights: High throughput imaging and high content screening of organoids
Advances in biomedical imaging technologies have significantly expanded the ability of researchers to study complex biological systems at cellular and subcellular resolution. In particular, three-dimensional (3D) organoid models have emerged as powerful experimental systems for investigating tissue …
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CRISPR-based approach enables targeted random nucleotide diversification in mammalian cells
… systems, preventing the evolution of proteins in higher organisms in their cellular context. The only tools available for mammalian cells rely on the AID cytidine deaminases, able to promote only G and C nucleotide diversification. Here we present hEvolvR, a CRISPR/Cas-guided error-prone DNA …
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Developing a clinically applicable hydrogel for the treatment of liver disease
… clinical need. To achieve these objectives, a high throughput imaging platform was first developed to identify critical extracellular matrix proteins involved in the maintenance of stem cell pluripotency and hepatocyte differentiation. Next, a selection of hydrogel-forming natural proteins was …
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Spectral modelling of molecular nitrogen in Aurora
… installed and operated with the HiTIES (High Throughput Imaging Echelle Spectrograph) at the Nordlysstasjonen in Svalbard, Norway (78.2025N and 15.829E). The filter provides images of the resonant scattered spectra of N+2 1N (0,1), (1,2), (2,3) bands and a neutral N2 2P (0,3) band. Ab …
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Identification of regulators in autophagosome formation using image-based siRNA screening
… we conducted a genome-wide siRNA screen using a high-throughput imaging system to identify undiscovered regulators in autophagosome formation. In this study, HEK293 cells stably expressing GFP-DFCP1 (GFP-tagged zinc finger FYVE-type containing 1) were used and amino acid starvation was used to …
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Evaluating Estrogen Receptor Signaling in Pancreatic β-Cells
… in pancreatic β-cell death as observed through high throughput imaging analysis and apoptotic endpoint assays (caspase 3/7). ERα46 expression resulted in increased transcriptional and caspase 3/7 activity similar to ERα66. Interestingly, in all assays ERα46 was more sensitive to ligand treatment …
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A Mechanism for Spatial Orientation Based on Sensory Adaptation in Caenorhabditis Elegans
… chemotaxis circuit, aided by a newly developed imaging system with flexible stimulus delivery and elevated throughput. I found reliable sensory responses to the behaviorally relevant range of odor concentrations. I then followed odor-evoked activity to downstream interneurons that integrate …
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Alkyladenine DNA glycosylase (Aag)-dependent cell-specific responses to alkylating agents
… of cell death after drug treatment by high-throughput imaging. CGNs isolated from WT, Aag-/-, and mAagTg mice exhibited cell sensitivity to MMS treatment that was dependent on Aag and Parp activity, thus recapitulating in vivo results and proving that CGN death is cell-intrinsic. Cell …
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Volumetric Imaging Across Spatiotemporal Scales in Biology with Fluorescence Microscopy
… them incapable of simultaneously achieving high spatial and temporal resolution. This thesis will describe the development of novel microscopy techniques and complementary computational tools capable of addressing some of the aforementioned limitations of fluorescence microscopy and further …
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Epigenetic Determinants of CRISPR-Cas9 genotoxicity
… Genotoxicity Assessment (MEGA) screen, a high-throughput imaging-based platform designed to identify multiple genotoxicity endpoints. These endpoints can be quantified and provide an assessment of how genotoxicity is impacted. The inclusion of the DNA methyltransferase inhibitor RG108 …
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Genomics and host response of S. Typhi infections
… and 1.1.0. All in all, this part of the thesis highlights the importance of better genetic surveillance in Latin America. In Chapter 5, I investigated the cell-specific responses of human induced pluripotent stem cell (hIPSC)-derived intestinal organoids and human gallbladder organoids to …
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Smart microscopy methods for quantitative microbiology
… has revolutionised this field by enabling high-throughput, non-invasive, time-lapse observations of cellular processes at the single-cell level. However, despite its power, this method is not without limitations. Optical artefacts distort quantitative measurements, obscuring the accuracy …
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High throughput platform for multiscale quantitative phase imaging
Quantitative phase imaging (QPI) yields the spatial phase map of the object’s scattering potential. QPI has enabled unprecedented label-free studies in biomedicine, ranging from cell dynamics and growth to cancer diagnosis and prognosis. The field is currently transitioning from technology-driven …