Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"High throughput assay"”.
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Developing a spectroscopy-based high throughput assay for anthocyanin content in corn
… Food & Drug Administration (FDA) and hence, are highly sought after as natural color pigment for the food industry. However, anthocyanins are susceptible to gradual degradation when exposed to certain food processing techniques or even due to prolonged/improper storage of food products that …
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Development and Application of a High-Throughput Assay for Discovery of Starch Hydrolase Inhibitors
For in vitro diabetes assay, to search for the active ingredients in low Glycemic Index foods, a 96-well based high-throughput method (HTS) for rapidly measuring the inhibition of starch hydrolase was developed, by monitoring the decrease in turbidity over time during the enzymatic digestion of …
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The development of a model system and high throughput assay for the study of interactions between zinc finger proteins and DNA
… to the elucidation of a code with a single stage high throughput screening assay. The work outlined here describes the development of a model system for the study of DNA protein interactions and the development of a high throughput assay for detection of such interactions. A zinc finger protein …
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A tractable nematode model for the emerging fungal pathogen, Candida auris
… are needed, especially those amenable to high-</p> <p>throughput analysis. The nematode <em>Caenorhabditis elegans</em> has been validated as an effective</p> <p>tool for studying multiple fungal and bacterial pathogens. In this thesis, I describe a <em>C. elegans</em></p> <p>infection …
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Trehalose Monomycolate Biosynthesis and Utilization in Mycobacterium tuberculosis
… difficult to treat is its extremely hydrophobic, highly impermeable cell wall, most notably characterized by the presence of long chain, ¿¿-branched mycolic acids. The Antigen 85 (Ag85) family of enzymes catalyze the transfer of mycolic acids from a single donor, trehalose monomycolate (TMM), to …
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Engineered Oligosaccharyltransferases With Unique N-Glycosylation Site Preferences
… against XXNXT library were built through a high-throughput assay. Two engineered OSTs combining distinct transmembrane domain and periplasmic domain of PglBs from C. jejuni and D. desulfuricans was also investigated. And the study interestingly revealed they can lose all their glycosylation …
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Development of chemical inducers of dimerization for screening competitive histone deactelyase inhibitors
… done using an in vitro fluorescence-screening assay, which is unable to test for selectivity. Alternatively, HDAC inhibitors are screened against commercially produced proteins, which is very time consuming and can be prohibitively expensive. To improve the screening methods for HDAC …
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Exploiting novel antibodies for the early detection of cardiac disease
… elevated levels of cardiac troponin I (cTnI) are highly-specific for cardiovascular damage. Using a specific targeting strategy, a panel of novel antibodies were successfully generated against key epitopes in cTnI that are of paramount importance for specific cardiovascular disease detection. …
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INSIGHTS INTO POLYMERISATION OF FTSZ AND OTHER CYTOMOTIVE FILAMENTS
… ZapA. I also present the development of a high- throughput assay for detection of better FtsZ inhibitors, which uses principles derived from the structural studies. Finally, I demonstrate that the conformational switch upon polymerisation seen in FtsZ is conserved within the tubulin …
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The prevalence of toxin-antitoxin systems and their tractability as novel antimicrobial targets
… therefore further investigated. A cell-based high-throughput assay was developed to screen for small molecules that induce toxin-dependent cell death in E. coli expressing RelBE. Additionally, mechanistic studies were performed to determine the mode of action by which the Txe toxin causes cell …
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Modeling the Fourier transform near infrared spectra of unreacted starch in corn for monitoring applications
… and (3) room temperature of 20°C. In addition, a high throughput assay based on the Fourier transform near infrared spectra of starch blends and corn flour was developed to predict unreacted starch contents in corn. Results of the study showed unreacted starch content in corn increased during the …
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Combinatorial lipid-like materials for intracellular delivery of small RNAs that activate innate antiviral immune responses and adjuvant vaccines
… immunostimulatory RNA (isRNA), a novel in vitro high- throughput assay was developed to screen the lipidoid library for delivery of isRNA.
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Quantitative modeling and control of nascent sprout geometry in in vitro Angiogenesis
… of nascent vessel growth in 3D microfluidic assays, formulate a quantitative process model based on our experimental characterization, and formulate a feedback approach to regulate growth. We begin by developing a new microfluidic assay consisting of a collagen gel scaffold with features to …
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Enhanced polymeric nanoparticles for gene delivery
… in the presence of serum proteins and have high gene delivery without toxicity to human primary cells. The biophysical properties of PBAE/DNA nanoparticles have good correlation to transfection efficacy when tested in the appropriate media conditions. We also show that electrostatic …
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Investigation of the molecular basis of PAMP-induced resistance
… to Pto DC3000. For this, I developed a high-throughput assay for bacterial infection in Arabidopsis seedlings that enabled to select mutants impaired in flg22-induced resistance to Pto DC3000. The pir (PAMP-induced resistance) screen identified four loci whose mutation leads to a …
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Genetically engineered sensors for non-invasive molecular imaging using MRI
… to image deep inside tissues at relatively high spatial and temporal resolution. However, the range of molecular phenomena currently accessible to MRI is limited by a lack of suitable molecular sensors. Most efforts to create such sensors have focused on synthetic contrast agents, whose …
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The specificity and evolution of gene regulatory elements
… by 30 UTR length. Finally, I introduce a new, high-throughput assay for the quantification of transcription factor in vitro binding affinity to millions of sequences. I apply this method to GCN4, a yeast transcription factor, and reconstruct all known properties of its binding preferences. …
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Genomic Approaches for Pathway Identification in Regenerating Sensory Epithelia of the Inner Ear
… affects on proliferation using a 96 well high throughput assay and profiled gene expression changes that resulted from each knockdown. Using these techniques we have determined genes required for SE proliferation and identified novel epistatic relationships between many of these genes. In …
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Evaluating Chemical Toxicity: A Novel Behavioral Assay Using Daphnia
… of their toxicity. There is a need for high-throughput assays to rapidly assess the toxicity of these emerging contaminants. A behavioral assay utilizing freely swimming Daphnia pulex was developed to evaluate the sub-lethal chemical effects. Daphnia, a keystone species, are small …
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Development And Optimization Of The First High Throughput In Vitro Fret Assay To Characterize The Saccharomyces Cerevisiae Gpi-T
… AND OPTIMIZATION OF AN IN VITRO FRET ASSAY TO CHARACTERIZE THE SACCHAROMYCES CEREVISIAE GPI TRANSAMIDASE</p> <p>By</p> <p>SANDAMALI AMARASINGHA EKANAYAKA</p> <p>December 2013</p> <p>Advisor: Dr. Tamara L. Hendrickson</p> <p>Major: Biochemistry</p> <p>Degree: Doctor of Philosophy</p> …
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