Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"High resolution melting"”.
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Application of High Resolution Melting analysis for haplotype detection in phylogeographic research and case studies of Arenaria ciliata, A. norvegica and Minuartia recurva (Caryophyllaceae)
… of phylogeography. First, the technology of High Resolution Melting (HRM) analysis based on real-time PCR is introduced as a tool for haplotype detection in phylogeographic research. Second, phylogeographic study of three arctic-alpine or alpine plant species in the family Caryophyllaceae is …
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Malignant hyperthermia: allele specific expression and mutation screening of the ryanodine receptor 1 : a dissertation presented to Massey University in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biochemistry
… wild type allele was found to be expressed at higher levels than the mutant RyR1 allele. These differences were not caused by variations in RYR1 mRNA stabilities. Secondly, high-throughput amplicon sequencing was employed for the quantification of both the T4826I and H4833Y causative MH …
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Optimization of Molecular Beacon-Based Multicomponent Probes for Analysis of Nucleic Acids
… followed by measuring ?Tm, the difference in melting temperatures of matched and mismatched hybrids. The approach enables SNS differentiation at elevated temperatures (usually 40-65oC) often only in a narrow range of < 10oC and requires high-resolution melting devices. Here we demonstrate that …
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Genomic analysis of the diapause program in the West Nile virus vector Culex pipiens.
… the male phallosome (genetalia). Here we offer high resolution melting curve analysis which allows the use of fixed, single nucleotide polymorphisms (SNPs) to cheaply and quickly validate the identities of mosquito specimens. Isolating and disruption of genes contributing to the life strategies …
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Discovery and characterisation of viral-like sequences and viruses associated with Phytophthora pluvialis in New Zealand
… pluvialis genotypes, a diagnostic method using high-resolution melting (HRM) analysis was developed and used to track horizontal transmission of PplRV1 between P. pluvialis isolates. The HRM results revealed PplRV1b was potentially transmitted between the isogenic isolates of P. pluvialis NZFS …
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Sviluppo di marcatori molecolari per il miglioramento genetico dei tratti qualitativi del frutto in uva da tavola.
… fenotipica, utilizzando approcci sia high-throughput che low-throughput, delle principali caratteristiche qualitative morfologiche, chimiche e fisiche del frutto su una popolazione segregante di uva da tavola risultante dall'incrocio delle cultivar ‘Italia’ e ‘Crimson Seedless’. …
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ROCK2 Polymorphism in Patients with Vasospastic Angina
… across the gene region, and then genotyped by high-resolution melting. Linkage disequilibrium and haplotype analyses were performed by using SHEsis program. The incidence of coronary risk factors, including age, sex, dyslipidemia, hypertension, diabetes, cigarette smoking and the polymorphisms …
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Bionomics of vector-borne diseases in sites adjacent to lakes Victoria and Baringo in Kenya
… Plasmodium parasites and arboviruses. This study highlights the presence and relative importance of zoonotic VBPs in both study areas.
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Detección de un polimorfismo marcador de sexo mediante CRISPR-CAS 12A en salmón coho
… asociado a un ensayo de enzima de restricción y “high resolution melting” (HRM), no obstante requieren de equipos especializados y en algunos casos son muy laboriosas de implementar en la práctica. El sistema CRISPR-Cas permite la detección de nucleótidos utilizando el mecanismo de acción de la …
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Classification of ornamental accessions of Miscanthus with molecular techniques
… accessions within the United States. 1) High Resolution Melting analysis was used to determine if accessions of Miscanthus could be distinguished at the species level. HRM was able to distinguish species and furthermore, it was found that material provided as M. x giganteus ‘Kurt Bluemel’ …
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Evaluation and adaptation of molecular approaches for detection and characterization of viruses of the respiratory tract
… of pathogenic viruses of the respiratory tract. High Resolution Melting (HRM) analysis was used in this study for screening similar sequences to avoid unnecessary sequencing from genomic libraries and detection of common pathogenic viruses of the respiratory tract. Four different structures of …
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Caracterização do perfil de resistência a antibióticos de isolados de Salmonella enterica obtidos na cadeia produtiva de aves
… resistência a ciprofloxacina) foram submetidos a High Resolution Melting (HRM) para detecção de mutações e os produtos de amplificação foram posteriormente sequenciados para caracterizar as mutações identificadas. A atividade de bombas de efluxo foi mensurada pela avaliação em ágar da exportação …
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Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health
… issue was addressed through the use of post-qPCR high resolution melting (HRM) analysis, providing a proof-of-concept for this approach, using 3 closely related Aspergillus, i.e., A. versicolor, Aspergillus creber and Aspergillus sydowii. This HRM tool will allow a more accurate monitoring of …
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Optimalizace izolace DNA z šípků
Plod růže šípkové (Rosa canina) nachází využití v potravinářském průmyslu, konkrétně při výrobě čajů. Plody šípku jsou bohaté na různé látky, jako např. fenolické kyseliny, které působí jako kontaminanty při izolaci rostlinné DNA. Tato práce má za cíl upravit CTAB protokol používaný k izolaci …