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Showing 1 to 20 of 70 for “"High pressure liquid chromatography"”.
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High pressure liquid chromatography of nucleotides and nucleic acid bases from fungi
High resolution liquid chromatographic methods have been developed for the determination of nucleotide pools at the nanogram level in four representative species of ascomycetes (P. citrinum, A. niger, F. moniliforme, and C. herbarum). Nucleotides were extracted from the mycelial mat in high yield …
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High Pressure Liquid Chromatography Studies of the Reaction of Platinum Complexes with Peptides
Platinum complexes (cisplatin, carboplatin and oxaliplatin) are effective anticancer agents. However the major drawbacks of platinum chemotherapy are toxic side effects and resistance. The affinity of platinum complexes to sulfur donor ligands of side chains of methionine and cysteine amino acids …
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Use of High Pressure Liquid Chromatography to Detect and Quantify Oxytetracycline Marks in Walleye Otoliths and Dorsal Spines
… The goal of this study was to determine if high pressure liquid chromatography (HPLC) is a feasible and superior alternative for detecting and quantifying OTC in otoliths of fry-marked and spines of fingerlingmarked walleyes. Study objectives were to validate methodology of extraction of OTC …
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Investigation of the Chemiluminescent Reaction Between Lucigenin and Organic Reductants and Its Application as a Detector for High Pressure Liquid Chromatography
The reaction of basic lucigenin (N,N'-dimethyl-9,9'-biacridinium dinitrate) with organic reductants was investigated as an analytical tool. Investigation of this reaction was carried out in three distinct steps: (1) stopped flow investigation of the reaction parameters; (2) development of a flow …
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Constituents of Aplysina Fistularis
… Aplysina fistularis, were examined by gas chromatography/mass spectrometry (GC/MS), high pressure liquid chromatography (HPLC) and classical fractionation and purification procedures. GC/MS indicated that many brominated tyrosine metabolites were accumulated in two A. fistularis samples, …
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Analysis of rubber vulcanisates
A study has been made, using High Pressure Liquid Chromatography, of the migration of TMQ (a quinoline type) and 6PPD (a paraphenylenediamine type) antidegradants from a tyre sidewall compound into adjacent casing and liner compounds containing no antidegradant. Migration takes place at a rapid …
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ΔΙΑΧΩΡΙΣΜΟΣ ΣΤΟΙΧΕΙΩΝ ΜΕΤΑΠΤΩΣΕΩΣ ΜΕ ΥΓΡΗ ΧΡΩΜΑΤΟΓΡΑΦΙΑ ΥΨΗΛΗΣ ΠΙΕΣΗΣ
… OF A NUMBER OF TRANSITION METALS BY HIGH PRESSURE LIQUID CHROMATOGRAPHY (HPLC). THE SEPARATION OF NI, CO, FE, CR, MO, V AND W FIRSTLY AS CHLORIDES AND SECONDLY AS CHELATES WAS STUDIED. SEPARATIONS OF NI/CO DIETHYLDITHIOCARBAMATES MO/CO/CR OXINATES NI, V/CR THIONATES WERE OBTAINED. THE …
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Electrochemical speciation and quantitative chromatography for modelling of indium bioleaching solutions
… ions, as well as with electrowinning additives. High pressure liquid chromatography techniques were adapted to quantify polysulfides (Sx2–) and metal ions in leaching and extraction solutions. This cumulative information was used in geochemical modelling. Predominance, Pourbaix, and speciation …
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Methods to Improve Marker Assisted Selection (Mas) for the Sugary Enhancer1 (Se1) Trait in Maize
… glucose and fructose were quantified using high pressure liquid chromatography (HPLC). The data indicate families and years are significantly different, and there is a strong family x year interaction for all sugars measured. Data showed a bimodal distribution among sucrose and fructose …
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Synthesis and Catalytic Decomposition of a Lipid Hydroperoxide
… be studied. Analysis of the reaction progress by high pressure liquid chromatography indicates a loss of lipid hydroperoxide and an increase of decomposition products. Spectral data for the ultra-violet active decomposition components have been collected. These results are compared to the latest …
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The Effects of Certain Oral Bacteria on the In Vitro Growth of Bacteroides gingivalis
… by use of a satellite phenomenon study and in a liquid medium by use of an optical density study. Incubation was conducted under aerobic conditions for seven days at 30°C.</p> <p>The sterile filtrates of trypticase soy broth which was used as the culture medium during incubation and growth of the …
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Neurochemical Levels Correlate with Population Level Differences in Social Structure and Individual Behavior in the Polyphenic Spider, <em>Anelosimus studiosus</em>.
… antagonistically with octopamine. We used High Pressure Liquid Chromatography with Electrochemical Detection to quantify levels of these neurochemicals among adult females from social (multi-female) and solitary (single-female) webs in east Tennessee. A subset of spiders was scored for …
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Surfactants and enhanced oil recovery
… sulphonated derivatives were given especially high priority. The surfactants were well-characterised in order to understand their EOR potential. High pressure liquid chromatography, mass spectrometry, Raman spectrometry, nuclear magnetic resonance spectrometry and other quantitative techniques …
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LC-MS/MS determination of various drugs of abuse and metabolites in municipal wastewater effluent samples
A method was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the quantification and confirmation of 8 drugs of abuse (cocaine, codeine, MDMA, methadone, methamphetamine, morphine, nicotine, and oxycodone) and their various metabolites (acetylmorphine, cotinine, EDDP, …
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Enzyme Kinetics of Recombinant Human Arylsulfatase B (rhASB)
… to a direct hydrolysis assay analyzed by High Pressure Liquid Chromatography (HPLC), this gives two potential methods of examining the kinetics of rhASB with both a monosaccharide substrate and the natural intracellular substrate</p>
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Photochemistry of Polycyclic Aromatic Hydrocarbons with Environmentally Relevant Metals
… simulator and resulting samples are analyzed by high-pressure liquid chromatography (HPLC), UV-Vis, attenuated total reflectance Fourier transformer infrared spectroscopy (ATR-FTIR), solid-phase extraction (SPE), and electrospray ionization/atmospheric pressure chemical ionization mass …
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Mouse Embryo Development in the Presence of Capsaicin
… with 1% ethanol and from 1 to 3mM capsaicin. The higher levels of capsaicin significantly inhibited embryo development. Post implantation fetuses were treated in the dam with 0.3, 0.6, 1.5, and 3 μmoles capsaicin, and tested for developmental defeds. No significant differences were found between …
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Η ετερογένεια των γ-αλυσίδων σε περιπτώσεις με αυξημένη εμβρυϊκή αιμοσφαιρίνη
… alkali denaturation and further characterized by high pressure liquid chromatography. Data for the three types of γ-chain were compared with those obtained for the HbF from previously reported black and nonblack patients with similar conditions. The ᴳγ-chain levels in Greek β-thal. heterozygotes …
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Natural and synthetic bis-indole alkaloids as cytotoxic agents
… of active compounds was attempted by open column liquid chromatography with both alumina and silica gel supports and by thin layer and high pressure liquid chromatography on silica gel. A compound was eventually isolated and purified and it proved to be a new bis-indole alkaloid. It was given the …
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