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Showing 1 to 16 of 16 for “"High performance thin layer chromatography"”.
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Characterisation of sandalwood essential oils using high performance thin-layer chromatography and high resolution gas chromatography mass spectrometry
… and oil yield have been observed both within and across Santalum species. Gas Chromatography-Mass Spectrometry (GC-MS) is a technique commonly used to identify and quantify sesquiterpene compounds in sandalwood essential oils. High Performance Thin-Layer Chromatography (HPTLC) has yet to …
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Prey-mediated effects of imidacloprid on Laricobius nigrinus (Coleoptera: Derodontidae) and Sasajiscymnus tsugae (Coleoptera: Coccinellidae), two predators of hemlock woolly adelgid
… enzyme linked immunoassay (ELISA) kit and a high performance thin-layer chromatography technique for detecting and quantifying imidacloprid residues in hemlock wood and needle tissues. ELISA is advantageous because of its cost, sensitivity, and ease of use. However, matrix effects in the form …
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Quantitative HPTLC
Advances in thin layer chromatography (TLC), including smaller more uniform particles, use of a scanning spectrophotometer (densitometer), and sample application devices, led to the development of the High Performance Thin Layer Chromatography (HPTLC) technique. HPTLC allows quantitative as well as …
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Human brain lipid fatty acid composition in relation to infant diet
… was analysed in these tissues by gas liquid chromatography after extraction and derivatisation. The subjects were divided according to their dietary history, either breast or formula feeding. Formula-fed infants were further subdivided according to the content of -linolenic acid in the …
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Changes in muskmelon perisperm envelope tissue during germination
… melo</i> L.) embryos are surrounded by a single layer of endosperm and a two- to four-cell-layered perisperm. The embryonic axis must penetrate the perisperm envelope (perisperm + endosperm) for germination to occur. Radicle emergence could result from increased turgor, weakening of the perisperm …
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Carbohydrates in Glycine max (L.) Merr. fruits during early ontogeny
… in the young developing fruits were found to be high at anthesis and then decreased with fruit growth. However, 6-8 DAF, fruits 7-9 mm showed an influx of soluble sugars and an accumulation of starch. Glucose was the major component of the soluble sugars in the 80% ethanolic extracts analyzed by …
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Flight activity and hemolymph diacylglyceride concentrations in Heliothis zea (Boddie) (Lepidoptera:Noctuidae)
In this study, a sensitive high performance thin-layer chromatography method (HPTLC) was developed to detect lipids in microgram (ug) quantities of hemolymph. The effect of age, diurnal rhythms, and flight activity on hemolymph 1,2-diacylglyceride concentrations in male and female Heliothis zea …
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Potential sources for the large scale production of human protein C
… production and downstream recovery of highly purified and biologically active hPC. The partial characterization of the protein with respect to post-translational modifications which are essential for functionally active, was also evaluated. Several studies were undertaken: 1. Cohn …
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Influence of flight activity and octopamine on hemolymph trehalose titers in Heliothis zea (Boddie) (Lepidoptera: Noctuidae)
… and female Heliothis zea were quantitated by high performance thin-layer chromatography (HPTLC) at various ages and times of the day and related to flight activity. Effects of octopamine injection or stress on trehalose levels were also quantified. Flight activity was measured with a …
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Thermal, oxidative and hydrolytic stability of selected frying shortenings evaluated by new and conventional methods
… and sensory. Three were new: contact angle; high temperature; and high-temperature gas chromatographic analysis of triglyceride; and polar component % as determined by high-performance thin-layer chromatography (HPTLC). Under static heating conditions, varying heating periods or shortening …
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A nutriceutical approach to inhibiting H1N1 influenza infection
… block virus infectivity but only at relatively high HMO concentration. The inhibitory concentration necessary to achieve 50% inhibition of infectivity was ~10 mg/ml for all HMO tested with the exception of 3-fucosyllactose (~5 mg/ml); a concentration likely far exceeding what would be feasible …
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Analysis of two single trait loci affecting flavonol glycoside accumulations in Arabidopsis thaliana natural variations
… derivatives in A. thaliana seedlings. First, high performance liquid chromatography photo diode array coupling with electro spray -ionization mass spectrometry (HPLC-PDA-ESI-MS) was employed to identify molecular mass of flavonoid aglycones and conjugate sugars. Second, high performance thin …
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Respiration rates and Factors which Influence the levels of Carbohydrates and Lipids in Honey Bee Eggs (<I>Apis mellifera </I>Linnaeus)
… and laying workers on day 1, 2 and 3 using high performance thin layer chromatography. The mean respiration rate for fertilized and unfertilized eggs from queens was 0.1 ± 0.0 µL CO₂/hr/egg, the same as the mean respiration rate obtained for unfertilized eggs from laying workers. The results …
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Investigations of Colorado potato beetle [Leptinotarsa decemlineata (Say)] pest management including: sampling strategies for insecticide resistance detection, development of a knowledge-based expert system and the physiology of cold tolerance
Within-field variation in mortality of Colorado potato beetle, Leptinotarsa decemlineata (Say), in a filter-paper insecticide bioassay was determined in three Virginia potato fields in 1989 and five fields in 1990. Bioassays were performed for each of three different insecticides on Colorado potato …
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Phytochemical, biological and toxicity studies of terminalia sericea burch. (Combretaceae)
… initially fractionated using silica gel column chromatography, where after, some of the fractions were further purified using silica gel and Sephadex LH-20 column chromatography. Final purification of the enriched fractions was achieved using preparative high performance liquid chromatography …