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Showing 1 to 20 of 678 for “"High affinity"”.
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Analysis of soluble high-affinity PD1 variants
Despite achieving significant clinical impact in several major cancer types such as melanoma or Hodgkin’s lymphoma, immunotherapies have not had much success in the treatment of pancreatic cancer. This cancer is characterised by a pro-inflammatory and immunosuppressive stroma which causes …
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Design of a high-affinity carbohydrate binding protein
Kohlenhydrat-Protein Interaktionen sind in der Natur weitverbreitet. Sie stellen die Grundlage für viele biologische Prozesse dar, wie zum Beispiel Immunantworten, Wundheilung und Infektionsprozesse von pathogenen Viren oder Bakterien mit einem Wirt wie dem Menschen. Neben der Infektion von …
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Structure Based Drug Design of High Affinity Kras Inhibitors
… that bind to mutant KRAS, we conducted a high-throughput virtual screen of drug-like compounds against a previously characterized allosteric pocket on a molecular dynamics simulation-derived KRASG12D structure. The in silico predicted hits were then validated with a battery of cell-based …
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Engineering Soluble, High Affinity Receptor Antagonists for Bacterial Exotoxins
… the engineering of murine Vbeta8.2 for picomolar affinity to staphylococcal enterotoxin B (SEB). As the known contact regions had already been heavily mutagenized, additional engineering of the Vbeta was performed through extension of the CDR1 loop. Soluble forms of the high-affinity Vbeta regions …
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Engineering and Characterization of High-Affinity T Cell Receptors
In Chapter 6, the role of TCR affinity in T cell activation was studied using CD8-negative T cells, as well as T cells expressing the co-receptor CD8. The peptide sensitivity of T cells that expressed the highest-affinity TCRs reached a plateau, suggesting the existence of an affinity maximum for T …
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The High Affinity Anti-Fluorescyl Immunoglobulin-M Response in Rabbits
… were initiated to determine the inducibility of high affinity IgM populations in the fluorescein hapten system. Fluorescein has characteristically elicited IgG antibodies of relatively higher affinity than values observed with other hapten systems. Characterization of protein-ligand interactions, …
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Searching for M13 bacteriophage with high affinity for nanodiamond particles
… vacancy centers to find phage that have an affinity for these nanoparticles. The phage DNA was sequenced and found to have the protein sequence SKMYHTP. At this point, although we have sequences of peptide that bind to nanodiamond, we are unable to determine the affinity or best binders …
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Kinetic Analysis of High Affinity Polyclonal Anti-Fluorescyl Immunoglobulin-G Antibodies
Mathematical and experimental tools for the measurement and assessment of association and dissociation kinetics in anti-fluorescyl antibodies were developed in these studies. An analytic procedure for the characterization of heterogeneous first order kinetics was derived. This procedure was based …
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Biophysical characterization of high affinity engineered single chain Fv antibody fragments
High affinity antibody binding interactions are important for both pharmaceutical and biotechnological uses. However, designing higher affinity interactions has remained difficult. Both high affinity interactions from nature and the results from directed evolution affinity maturation processes may …
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MYO1C Binds to PiP2 With High Affinity Through a Putative Ph Domain
… a non-cooperative manner. It binds with slightly higher affinity to Ins(1,4,5)P3 as well as other inositol phosphates which may act as inhibitors to membrane binding in the cell. By sequence and secondary structure analysis, we identified this phosphatidylinositol binding site in the tail to be a …
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Novel therapies for cancer treatment : designing high affinity selective ligands against SIRT1 enzyme
… “How can SIRT1 behave as a tumour suppressor?”, highly selective ligands (aptamers) were developed against SIRT1 enzyme as the first step towards the development of an alternative chemotherapy for cancer diseases. The objectives of current study are to: (i) produce by in vitro SELEX procedures, …
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The Effect of Modifying the Higher-Order Structure of Plant High-Affinity Phosphate Transporters
… to cope with phosphate starvation, including high-affinity phosphate transporters (Phts). Phts are membrane-localized transporters involved in the transport of phosphate and arsenate and have a high capacity for transporting phosphate in areas where phosphate concentrations are low, behaving …
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Polymorphisms of High-affinity IgE Receptor Genes in Patients with Aspirin-Intolerant Chronic Urticaria
… chronic urticaria have autoimmune urticaria. The high-affinity receptor for IgE (FcεRI) expressed on mast cells and basophils plays a critical role in mediating allergic reactions induced by antigen-specific IgE antibody bindings. FcεRI is composed of three subunits: the IgE-binding α-chain, a …
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Binding, Thermodynamic and Structural Studies of High-Affinity T Cell Receptor-Peptide MHC Interactions
Finally, in chapter 5, fluorescence spectroscopy was used in stability and binding studies of scTCRs. Urea denaturation analysis was used to show that scTCRs engineered in the yeast display system have similar stabilities as single chain antibody Fvs. The yeast display system was successful in …
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Cryoprecipitation Properties and Structural Analysis of a High Affinity Anti-Fluorescein Immunoglobulin-M Antibody
A high affinity (K$\sb{\rm a} = 2.9 \times 10\sp $ M$\sp{-1}$) murine monoclonal anti-fluorescein IgM antibody (18-2-3) exhibiting low temperature insolubility in the absence of bound ligand has served as a model to study cryoprecipitation. Insolubility of 18-2-3 at low temperature (4$\sp\circ$C) …
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Building a Genetic System in Yeast to Search for High Affinity Proteins in Sequence Space
… step in discovering new binders is called affinity maturation, when an initial hit that shows some ability to bind the target is further mutated in additional steps to improve binding affinity, specificity, solubility, pharmacokinetic profile. Ideally, the methods for affinity maturation …
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Engineering soluble, high affinity T cell receptor domains for detection of staphylococcal and streptococcal exotoxins
… both cell types. Although SAgs bind with low affinity to the variable region of the beta chain (Vβ) of the TCR, they are very potent toxins. These toxins have been incriminated in food poisoning, sepsis, toxic shock syndrome (TSS), infective endocarditis, and skin conditions like atopic …
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Methylated DNA detection using a high-affinity methyl-CpG binding protein and photopolymerization-based amplification
… to evolve the hMBD2 protein for improved binding affinity. Affecting five amino acid substitutions doubled the affinity of the wildtype protein to 3.1+/-1.0 nM. Further, concatenating the high-affinity MBD variant from 1x to 3x and expressing it in Escherichia coli as a green fluorescent protein …
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