Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"HiSeq"”.
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Transcriptiome analysis of a migraine model
… Samples were sequenced using the Illumina HiSeq 4000 platform to produce paired end reads of 100 bp. Opalin, Slc32a, Cacna1b, and H2-Eb2 were among the 110 genes exhibiting significant interaction effects. The 165 genes presenting different expression patterns between the treatment and …
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Metagenomic Analysis of Antibiotic Resistance Genes in the Fecal Microbiome Following Therapeutic and Prophylactic Antibiotic Administration in Dairy Cows
… DNA extraction followed by Illumina paired-end HiSeq sequencing and quantitative polymerase chain reaction (qPCR). Illumina sequences were analyzed using MG-RAST and the Antibiotic Resistance Gene Database (ARDB) via BLAST. Abundance of ampC, ermB, tetO, tetW, and 16S rRNA genes were determined …
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Encoded synthesis and evolution of clinically approved 2'-modified ribonucleic acids
… followed by deep screening using an Illumina HiSeq instrument. By advancing these aptamer selections further, this study lays the groundwork for the development of the first MOE-RNA aptamer and demonstrate the potential of encoded synthesis of 2'-modified nucleic acids in creating biostable …
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Through the layers of the Ethiopian genome: a survey of human genetic variation based on genome-wide genotyping and re-sequencing data
… newly collected and sequenced on an Illumina HiSeq platform. These populations were chosen, from among the ones typed on the SNP array, to represent the main components of Ethiopian genetic diversity. Of the 25 samples per population, 24 were sequenced at low depth to generate a broad list of …
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Comparison of the molecular phenotypes of pigs carrying different IGF2 alleles at four developmental time points
… total 46 samples were sequenced on the Illumina HiSeq 2000 with a target depth of 20 million reads per sample. Analysis of the RNA-seq data using tweeDEseq determined differentially expressed genes between Apat and Gpat pigs at each time point. Additionally, IPA analysis was used to determine if …
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Studio in vitro dell'espressione di varianti del gene CFTR con ruolo patofisiologico non ancora definito
… sequencing (NGS) kit, using the platform HiSeq 2000 (Illumina), in order to scan the entire CFTR gene. After a careful evaluation of the possible alternatives, we chose a target enrichment strategy and designed a set of probes covering the full-length CFTR to be used for DNA capture …
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Error Correction and de novo Genome Assembly of DNA Sequencing Data
The ability to obtain the genetic code of any species has caused a revolution in biological sciences. Current technologies are capable of sequencing short pieces of DNA with very high quality. These short pieces of DNA determint the sequence of bases in the genome of any species. This information …
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Functional and community investigation of nodule endophytes for the selection of phosphate solubilizing rhizobial inoculants for clover : A thesis submitted in partial fulfilment of the requirements for the degree of Doctor of Philosophy at Lincoln University
… 16S rRNA gene amplicons sequencing (Illumina HiSeq) was used to characterise the bacterial communities of nodules, roots and rhizosphere of white and subterranean clovers, grown in different P fertilization histories. Bacterial communities were different in the two clover hosts and in the …
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Transcriptomic and metagenomic impacts of dietary energy of milk replacer in pre-weaned Holstein heifers
… a paired-end analysis at 100 bp using Illumina HiSeq 2500. Operational taxonomic unit (OTU) clustering analysis was conducted using the 16s rRNA Greengenes reference. A PERMANOVA analysis was conducted in R to determine OTU populations for age and treatment. There was no difference in microbiome …
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Comparing Differentiated Rod And Cone Transcriptomes Reveals Rax As A Controller Of A Defined Cone Gene Regulatory Program
… by RNA sequencing (RNA- seq) using Illumina Hiseq 1500 (n=3). Moreover, after overexpression of human Retina And Anterior Neural Fold Homeobox (RAX), using subretinal injection of AAV2/8 hGNAT1-hRAX2aeGFP at a dose of 5 x 10<sup>11</sup>gc/eye we studied the GRN associated with RAX, by a …
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The Epidemiology and Evolution of Rifampicin Mono Resistant Tuberculosis in Khayelitsha, Cape Town, South Africa
… whole genome sequencing (WGS) on the Illumina HiSeq. The raw fastq WGS data files of the sequenced DNA were securely transferred to UCT. TB profiler was used to identify RIF R conferring mutations in rpoB and strain lineages. rpoB mutations were classified as high/moderate and minimal …
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Whole exome sequencing: a customised approach to exploring the genetic basis of musculoskeletal soft tissue injuries
… Paired-end WES was performed on the Illumina HiSeq 2000/2500 platform at 30X coverage. A customised, tiered filtering strategy was developed to screen for candidate variants. All candidate variants were confirmed using Sanger Sequencing and genotyped, together with the other prioritised …
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Genetic and Maternal Factors Underlying Early Milk Production and Their Influence on Calf Health
… CHAR calves, n=13). Samples were run on the HiSeq 2500 platform as paired end reads according to Ilumina's standard sequencing protocol. A regression analysis was done in SAS using PROC GLM and regressing mode of birth on milk components for d 1,3, and 28. After, milk components found to be …
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Implementación de técnicas de secuenciación masiva para el desarrollo de nuevos algoritmos diagnósticos y bioinformáticos en distrófias hereditarias de retina
… SOLiD 5500xl, Ion Torrent PGM) e Illumina (ej: HiSeq, MiSeq, NextSeq). El objetivo de la presente tesis doctoral es diseñar, validar e implementar una aproximación para el diagnóstico de las DHR basada en paneles de genes específicos de nuestra población y un sistema personalizado de los datos …