Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Hfq"”.
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Investigating Hfq-Mrna Interactions In Bacteria
… requires the action of an RNA binding protein, Hfq. Hfq facilitates these RNA-RNA interactions by stabilizing duplex formation, aiding in structural rearrangements, increasing the rate of structural opening, and/or by increasing the rate of annealing [18-21]. Hfq has two well characterized …
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Modes and mechanisms of hfq mediated stress regulation in bacteria
… gene expression. The RNA binding protein Hfq facilities this process by, helping sRNA to base pair with its target mRNAs to initiate gene regulation. A common feature of Hfq-mediated gene regulation is the network-based organization where a single sRNA can control multiple messages to …
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Mapping RNA Binding Surfaces on Hfq Using Tryptophan Fluorescence Quenching
<p>Abstract</p><p> Hfq is a pleiotropic posttranscriptional regulator and RNA chaperone that facilitates annealing of trans-encoded sRNA/mRNA pairs. It regulates many different cellular pathways including environmental stress responses, quorum sensing, virulence and maintenance of membrane …
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Identification and Characterization of the Hfq protein and small RNAs in Francisella novicida
… determine the role of the RNA chaperone protein Hfq and identify potential novel small RNAs in <italic>F. tularensis</italic> subsp. <italic>novicida</italic> strain U112. The Hfq protein is recognized as an important regulatory factor in a variety of cellular processes, including stress …
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Investigating Hfq-dependent Small Regulatory RNA, Bsr34, and Its Roles in Brucella abortus Physiology and Pathogenicity
… regulatory RNAs (sRNAs), often chaperoned by Hfq, are key post-transcriptional regulators of bacterial virulence and stress adaptation, yet remain largely uncharacterized in Brucella. Following the identification of Hfq-associated sRNA candidates in B. suis, this study provides the first …
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The Characterization of PG0228 in Porphyromonas gingivalis W83
… facilitation of the bacterial Sm-like protein, Hfq, for optimum function. Hfq is a RNA chaperone involved in RNA stability, sRNA function, and polyadenylation. Mutants lacking in Hfq often show pleiotropic phenotypes, although the extent and severity of hfq null phenotypes is often …
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Studying the riboregulatory role of Hfq in Klebsiella pneumoniae using CLASH (crosslinking, ligation and sequencing of hybrids)
… proteins (RBPs). In this study, I investigated Hfq, an RBP in a Gram-negative nosocomial pathogen Klebsiella pneumoniae (Kp), using a high-throughput sequencing technique named CLASH (Crosslinking, ligation, and sequencing of hybrids). I performed CLASH in two Kp strains - Ecl8 and Ecl8ΔramR …
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The role of post-transcriptional regulators in pathogenesis and secondary metabolite production in Serratia sp. ATCC 39006.
… was investigated. It was hypothesized that Hfq-dependent small regulatory RNAs (sRNAs) might also play a role. Hfq is an RNA chaperone involved in post-transcriptional regulation that plays a key role in stress response and virulence in other bacterial species. An S39006 ∆hfq mutant was …
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Towards Understanding Helicase and Chaperone Activities in the RNA Degradosome
… shown to associate with the RNA chaperone Hfq to form a small RNA guided machinery that targets defined transcripts. This thesis attempts to investigate several characteristics of the degradosome, including the importance of RhlB, the structure of a portion of the RNase E C-terminal domain …
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Fantastic ribonucleoprotein complexes and how to study them. Architectural principles of regulatory assemblies in bacteria.
… of gene expression. A key RBP in bacteria is Hfq, which is best studied as a facilitator for sRNA-mRNA pairing. In Pseudomonas aeruginosa, however, Hfq is known to modulate translation of more than 100 genes in cooperation with an effector protein, Crc, by sequestering the ribosome binding …
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An E. coli small RNA inhibits translation initiation from a distance
… initiating ribosomes while the chaperone protein Hfq plays a supporting role. Contrary to this widely accepted model, there are a few examples in the literature where the sRNA base pairs far from the SD region, yet translation of the target mRNA is still inhibited. Mechanistically, non-canonical …
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Investigation into the transcriptional regulation and role in virulence of the Type VI Secretion System of <em>Serratia marcescens</em> Db10
… of the Sma Db10 T6SS is under the control of Hfq, a global post-transcriptional regulator. Hfq plays a critical role in small (s) RNA mediated- regulation, acting as a chaperone to facilitate the interaction of sRNAs with their target mRNAs. Deletion of Hfq in Sma Db10 results in a severe …
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Secretion and regulation of the chitinolytic machinery in <i>Serratia marcescens</i>
… ChiR, a LysR-type transcriptional regulator, and Hfq, a RNA-binding protein, on Serratia chitinolytic machinery. Results revealed that Hfq plays a role on ChiR synthesis which modulates chitinase and chiWX transcription. In addition, overexpression of chiR resulted in a higher population of ChiC …
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The Response of Staphylococcus aureus to Culture in a Low-Fluid-Shear Environment
… decreased expression of the RNA chaperone gene, hfq, which parallels the low-shear response of certain Gram-negative microorganisms. This is the first report describing an Hfq association with fluid shear in a Gram-positive organism, indicating that the ability to sense and respond to mechanical …
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Developing high-resolution methods to study DNA and RNA dynamics
… via basepairing interaction with the guide of Hfq protein. Nevertheless, how individual nucleotides within sgrS sequence contribute to the regulation process is not clear. Here we utilized the recently developed Sort-Seq method combining fluorescence-activated cell sorting (FACS) and …
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Study Of Protein-Rna Interactions Using Fluorescence Resonance Energy Transfer (fret) And Single-Molecule Fret
… extended duplex. In the cell, proteins, such as Hfq, facilitate the formation of functional RNA structures. Here we show that Hfq acts as a chaperone to overcome high-energy barriers and promote the progression of kissing hairpins through strand displacement to an extended duplex formation.</p>
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The phenotypic and molecular responses of Listeria monocytogenes to stressors
… virulence potential. Inactivation of ctsR, hfq, lisR and lisK by site-directed mutagenesis gave rise to mutant cells with increased sensitivity to H2O2. Citric acid resistance was impaired by ctsR and hfq disruption. Pre-conditioning under oxygen limiting conditions significantly increased …
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A small RNA regulates RpoS in the Lyme disease spirochete Borrelia burgdorferi
… as, identified a non-canonical RNA chaperone Hfq. The alternative sigma factor RpoS (sigma 38 or sigma S) plays a central role in the reciprocal regulation of the virulence-associated major outer surface proteins OspC and OspA. Temperature is one of the key environmental signals controlling …
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