Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Heterologous proteins"”.
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Modulating Tumor Immune Microenvironment Using Heterologous Proteins
… Modulating the tumor microenvironment with heterologous non-human proteins opens avenues for enhanced therapeutic efficacies with a minimal chance of developing resistance . In this thesis we have explored therapeutic potential of engineered proteins derived from pathogenic bacteria …
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Modulating Tumor Immune Microenvironment Using Heterologous Proteins
… Modulating the tumor microenvironment with heterologous non-human proteins opens avenues for enhanced therapeutic efficacies with a minimal chance of developing resistance . In this thesis we have explored therapeutic potential of engineered proteins derived from pathogenic bacteria …
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Development of a Transformation System for Caldariomyces Fumago and Expression of Heterologous Proteins
… in the gene area. Sequences coding for the heterologous proteins ligninase and calcitonin were cloned into the site of the chloroperoxidase structural gene and the plasmids transformed into spheroplasts. Integration of the genes into the C. fumago genome was verified by polymerase chain …
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Engineering dual-glycan responsive expression systems for tunable production of heterologous proteins in Bacteroides thetaiotaomicron
… for the capability to produce functional heterologous proteins within a host intestine. To improve the strength and regulatory fidelity of transgene expression in B. theta, I have developed platform expression strains with engineered regulatory proteins under control of promoter elements …
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Cloning and characterization of the Pichia Pastoris PMR1 gene
… system, is limited in its ability to secrete heterologous proteins. The PMR1 gene, the disruption of which is known to improve the secretion of prochymosin, human prourokinase, and human tissue plasminogen activator in Saccharomyces cerevisiae, was cloned from P. pastoris. The pmr 1 mutant in …
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Characterization of the MATα pre-/pro- peptide by mutagenesis as a means to optimize secretion in pichia pistoris
… as one of the most popular host systems for heterologous proteins due to its relatively cheap cost, easy genetic manipulability, ability to perform post-translational modifications on proteins, and respiratory growth capabilities which allow it to be cultured in very high concentrations. Over …
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Production and preliminary characterization of a fusion protein comprising streptavidin and a cellulose-binding domain
… (CBDCex) from Cellulomonas fimi can be fused to heterologous proteins. The fusion proteins can be purified by affinity chromatography on cellulose or immobilized on cellulose (Ong et al., 1989b). Streptavidin, a protein produced by Streptomyces avidinii, binds to the water-soluble vitamin, …
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Cloning and characterization of MET2 in Pichia pastoris
… a protein expression system to express over 500 heterologous proteins. P. pastoris provides many advantages over other organisms that have been utilized for this purpose. In this project, we developed a new host/selectable marker and auxotrophic strains of P. pastoris based on methionine …
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Characterizing potential secretion components that increase secretion of recombinant proteins in Pichia Pastoris
… for its ability to produce and readily secrete heterologous proteins. Nonetheless, there are obstacles in making this useful yeast into a more efficient secretion system that readily secretes problem proteins. In the Lin-Cereghino lab, mutant strains were developed by the method of restriction …
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A directed evolution approach to engineering recombinant protein production in S. cerevisiae
… expression host for the manufacture of these proteins is a potential solution. Although heterologous proteins are frequently made in organisms as disparate as E. coli and bovines, the single-celled organism S. cerevisiae has emerged as a well-qualified candidate due to its approachable genetic …
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Characterization of components that increase secretion of recombinant proteins in pichia pastoris
… used for its ability to express and secrete heterologous proteins. However, some proteins are not readily secreted in P pastoris and so adjustments in the secretion pathway must be made in order to achieve secretion. The Lin-Cereghino lab previously developed mutant strains using restriction …
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Characterization of the 5̕ untranslated region ( 5̕ UTR) of the alcohol oxidase I (AOX I) gene in Pichia pastoris.
… The 5' UTR influences the expression of many heterologous proteins in P. pastoris. However, no systematic analysis has ever been performed on this region to date. Several truncated versions of the 5' UTR were constructed using the QuikChange II XL Site Directed Mutagenesis Kit from Stratagene, …
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USING ANTISENSE MESSENGER RNA TO DOWNREGULATE LON MEDIATED PROTEOLYSIS IN ESCHERICHIA COLI
… has instigated the introduction of foreign heterologous proteins into host cells, such as Escherichia coli. However, the metabolic burden incurred by the host cell to produce the desired recombinant protein elicits a cellular stress response that can result in reduced yields and degradation …
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Transient heterologous protein expression in green microalgae
Introduction: Recombinant proteins are produced using recombinant DNA technology and are widely used in numerous industrial applications, as well as innovative therapeutics. As the requirements of industries and pharma grow more complex chemically, traditional chemical engineering is, at times, …
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Use of the cellulose-binding domain of a cellulase from cellulomonas fimi for affinity purification of fusion preteins
… cellulase as an affinity tag for purification of heterologous proteins. The CBD of endoglucanase A (CenA) from Cellulomonas fimi is an N-terminal domain comprising amino acids. CenA binds strongly to cellulose, and the CBD retains this function when separated from its cognate catalytic domain by …
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Investigating the Roles of CwlD and GerS in <i>Clostridioides difficile</i> Spore Cortex Modification
… degradation may impede the expression of these heterologous proteins in B. subtilis. This contrasts with prior research demonstrating observable production of variants even though they remained non-functional in B. subtilis. These findings highlight the significance of the native cellular …
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Hybride Modellbildung zur Prozessführung von Fed-Batch-Kultivierungen mit Aspergillus niger
… for the industrial production of homologous and heterologous proteins. In this work, the growth and product formation of A.niger in fed-batch cultivations is modelled by using the enzyme glucoamylase as an example product. Since classical modeling of bioprocesses involves a great deal of effort, …
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Production and Secretion of Recombinant Human Fibrinogen by the Transgenic Murine Mammary Gland
… animals has several advantages for production of heterologous proteins including a high cell density that results in high concentrations of secreted protein and the ability to perform several types of post-translational modifications. Transgenes were constructed from the 4.1 kbp murine Whey Acidic …
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Structural characterization of the MATα prepro-peptide secretion leader in Pichia pastoris
… system for the production of recombinant proteins for biopharmaceutical or industrial purposes. P. pastoris has the ability to produce foreign proteins at high levels extracellularly, and since it secretes few endogenous proteins, this ability eliminates the need for expensive purification …
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Calcium homeostasis in Pichia pastoris
… the effects of calcium and magnesium on growth, heterologous protein expression, and calcium homeostasis in P. pastoris . The divalent cations calcium and magnesium are responsible for diverse roles in in the function of the cells in eukaryotes. Although it is known that calcium is responsible …
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