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Showing 1 to 7 of 7 for “"Heterologe Genexpression"”.

  1. Optimierung von Schizosaccharomyces pombe für die heterologe Genexpression

    … deutlich erhöhten Ausbeute des disulfidhaltigen heterologen Proteins Orf19p-HA führt. Dieser Befund impliziert, dass in S. pombe die Oxidation der Disulfidbrücken für die Faltung von Proteinen vermutlich limitierend ist.

    qucosa-diss

  2. Entwicklung von Fermentationsprozessen zur Produktion rekombinanter Antikörperfragmente in Pichia pastoris und Nicotiana tabacum

    Being partly funded by the EU-project "Processing technology for recovery of recombinant antibody produced in crop plants" (FAIR-PL-CT96-1039), the aim of this work was to provide approximately 500 mg of the recombinant single-chain antibody fragment 4813 to enable the development of a synthetic …

    aachen Repository record for Entwicklung von Fermentationsprozessen zur Produktion rekombinanter Antikörperfragmente in Pichia pastoris und Nicotiana tabacum (opens in a new tab)

  3. Rekombinante Herstellung und Charakterisierung phenoloxidierender Enzyme aus Geobacillus stearothermophilus zur Evaluierung einer biosensorischen Anwendung

    In the current thesis the genetic structure of the phenol hydroxylase of Geobacillus stearothermophilus has been clarified. The single components Phe A1 (oxygenase component), Phe A2 (Flavin reductase component) and a tandem construct consisting of both components were successfully produced with an …

    aachen Repository record for Rekombinante Herstellung und Charakterisierung phenoloxidierender Enzyme aus Geobacillus stearothermophilus zur Evaluierung einer biosensorischen Anwendung (opens in a new tab)

  4. Entwicklung eines Enzym-Modul-Systems zur Synthese und in situ Regeneration von dTDP-aktivierten Desoxyzuckern

    The results of this thesis showed for the first time that it is possible to produce glycosylated natural compounds in vitro with in situ regeneration of dTDP-activated deoxysugars. The deoxysugar biosynthesis genes from the avilamycine- (aviS, aviT), urdamycine- (urdR) and midekamycine-biosynthesis …

    aachen Repository record for Entwicklung eines Enzym-Modul-Systems zur Synthese und in situ Regeneration von dTDP-aktivierten Desoxyzuckern (opens in a new tab)