Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"Hepatoma cell"”.
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Defect of Mitochondrial Translation Machinery Enhances Hepatoma Cell Invasiveness
I. INTRODUCTION 1 II. MATERIALS AND METHODS 4 1. Cell cultures, drug treatment and cell growth rate 4 2. Reverse transcription-polymerase chain reaction (RT-PCR) 4 3. Endogenous cellular oxygen consumption rate 5 4. Estimation of intracellular and mitochondrial ROS level 5 5. Total genomic DNA …
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Regulation of mitochondrial respiration by LDHB suppression in hepatoma cell lines
… and mitochondrial respiratory defects in hepatoma cells. In this study, we aimed to investigate how LDHB suppression is linked with mitochondrial respiratory dysfunction. When LDHB expression was suppressed by siRNA-mediated knockdown, cellular oxygen consumption rate was significantly …
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Regulation of Phospholipid Transfer Protein Secretion and Gene Expression in the Hepatoma Cell Line, HepG2
Hep G2 cells were used as a model to study HDL distribution and interconversion corresponding to variations in PLTP activity. An increase in PLTP activity by butyrate treatment was accompanied by increases in the amount of HDL having a hydrated density of 1.10 g/ml, HDL particle size and the …
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Hepatitis B Virion and cccDNA Formation in Primary Tupaia Hepatocytes and Human Hepatoma Cell Lines upon HBV Genome Transduction with Replication-defective Adenovirus Vectors and in vivo Infection of Tupaias
… with factors on the surface and inside the host cell. To by-pass restrictions during the initial entry phase, recombinant replication-defective adenovirus vectors, either without or with a green fluorescent protein marker gene, were constructed and used to deliver complete HBV genomes into …
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Effect of mitochondrial respiratory defecton hepatocellular carcinoma :Involvement of mtOGG1 and NUPR1
Many solid tumor cells exhibit mitochondrial respiratory impairment. However, the mechanisms of such impairments in cancer development remain unclear. In this study, to investigate an effect of mitochondrial dysfunction on hepatocellular carcinoma, the two projects were studied. The first project …
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Coordinated Control of Liver-Specific Genes in Cultured Cells from Mice and Rats
… fresh livers, primary hepatocyte cultures, and hepatoma cell lines, derived from mice or rats. The transcription rates of many different liver-specific but no common mRNAs were found to decline sharply within 24 hours when hepatocytes from the liver were explanted and placed into primary …
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Roles of Interferon Lambda on Hepatitis B Virus Replication
… described IFN-λ family utilizing its distinct cellular receptor has been reported to exert inhibitory effect on replication of several viruses. In this study, I wanted to know whether IFN-λ1 inhibits HBV replication in human hepatoma cell lines and whether HBV modulates expression of CRF2-12, a …
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Development and analysis of an in vitro model of inflammatory cytokine-mediated idiosyncratic drug hepatotoxicity
… both directly and indirectly, in drug effects on cellular physiology. Our ultimate goal is to develop an in vitro model effectively mimicking certain relevant aspects of the in vivo response of the human liver. In our initial effort described herein, we have designed a novel cell-based system …
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Albumin Transcriptional Regulation
… proteins and others of a more ubiquitous cellular distribution. It is therefore, the interaction of several factors (only some of which are liver specific or enriched) which dictates the liver specificity of this promoter. The albumin enhancer element was localized much farther upstream …
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MOLECULAR BIOLOGY OF HEPATITIS B VIRUS DNA-CELL INTERACTION
… a ?gtll expression library constructed from the hepatoma cell line HuH-6 which was screened with a binding site probe derived from EII. Sequence analysis of E2BP revealed 86.6% homology with a rat hnRNP-C c-terminal protein sequence while conformational studies suggest a "helix-loop-helix'' motif …
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Mechanisms of action for aryl hydrocarbon receptor ligands in the PLHC-1 cell line
… and activity was characterized in the teleost hepatoma cell line, PLHC-1. This work was carried out in order to gain insights into mechanisms of halogenated aromatic hydrocarbon (HAH) toxicity. The results improve our ability to characterize the risks posed by HAH exposure as well as further …
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Do Elevated Levels of Glutathione Decrease the Toxicity of Plasticizers in Mouse Liver Cells?
… aims to investigate the response of two mouse hepatoma cell lines to the effects of DEHP and its bioactive metabolite mono (2-ethylhexyl) phthalate (MEHP). Cells were exposed to four concentrations (10, 50, 100, and 300 µM) of DEHP and MEHP, which are consistent with levels encountered in …
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Regulation of Cholesterol Biosynthesis in Hepatocytes
… natural compounds were screened using the human hepatoma cell line HepG2. One compound, berberine, showed great potential as a regulator of cholesterol synthesis and so became the subject of this investigation. Berberine inhibited HMG-CoA reductase activity and decreased cellular accumulation of …
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Preneoplastic hepatocyte hypothyroidism underlies hepatocellular carcinoma progression
Thyroid hormones elicit many cellular and metabolic effects. Most of them are mediated by the thyroid hormone 3,5,3’-L-triiodothyronine (T3) nuclear receptors (THRs), which act as transcription factors. THRs have also been implicated in tumorigenesis, although it is unclear whether they act as …
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The Intracellular Changes and the Localization of Core particles by Hepatitis B Virus P Protein-Deficient Mutant
… pregenomic RNA (pgRNA). In this study, the intracellular changes of P protein-deficient mutant expressing stable cells and the localization of P protein-deficient mutant core particles were observed. MATERIALS & METHODS: HBV replicating and non-replicating stable cells had been established in …
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Phenotypic characterization of a clinical HBV/G isolate relative to a co-infecting HBV/A strain and HBV/A/G recombinant strains
… and transiently transfected into the HepG2 human hepatoma cell line for phenotypic analysis of each strain. HBV/G, HBV/A and HBV/A/G recombinant strains were isolated from Patient 1, while a different HBV/A/G recombinant strain was isolated from Patient 2. HBV replication capacity was measured …
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Relationship between Hepatitis B Virus Core Particles and Intracellular Motility
… was observed in HBV replicating stable cell lines. Also, how HBV core particles were transported in cytoplasm was elucidated. MATERIALS & METHODS: HBV replicating stable cell lines were established in hepatoma cell lines, HuH7 and HepG2 cells. Using Northern blot and Southern blot …
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Studies on Taxol Resistance Induced by HBx Viral Oncoprotein
… taxol often fails due to resistance of hepatocellular carcinoma (HCC). I attempted to determine the effect of taxolon HCC.Infection with hepatitis B virus (HBV)is a high risk factor for the development of HCC.Hepatitis B virus X (HBx)protein encoded by HBV genome is likely to play a important …
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Hormetic dietary phytochemicals from Western Canadian plants: Identification, characterization and mechanistic insights
… from Western Canada for QR induction in a mouse hepatoma cell line (Hepa-1c1c7) led to the identification of twenty-one extracts capable of doubling the activity of QR. Bioassay-guided fractionation of six extracts led to the identification of novel classes of compounds with QR-inducing activity …
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