Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Hepatoblast"”.
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Generation and characterisation of primary and pluripotent stem cell-derived human hepatoblast organoids
… a method to derive and grow primary human hepatoblast organoids (HBOs) isolated from human foetal liver tissues. These cells accurately and reproducibly recapitulate the hepatoblast identity in vitro, as they possess self-renewal and differentiation capacities, like the in vivo counterpart. …
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Modelling human liver bud development in vitro
… liver bud. This includes a ‘bud’ containing hepatoblast progenitors (ALB+AFP+HNF4A+), and a ‘trunk’ containing hepato- pancreato-biliary progenitors (KRT19+CDX2+PDX1+HNF1B+) and extra-hepatic cholangiocytes (ACE2+MUC13+). By performing extensive snRNAseq analyses across HepBud formation, I …
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Creation of organoid systems to model human endodermal organ development in vitro
… based upon the archetypal liver stem cell, the hepatoblast, whilst the other was based upon a hitherto undescribed biliary progenitor cell. The work continued with exploration of the development of the human intestine, characterising how intestinal organoids develop in vitro, and going on to …
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Production of hepatocytes from human pluripotent stem cells for cell-based therapy
… optimum time points for each step especially for hepatoblast and foetal hepatocytes stage. Overall, the work presented in this dissertation has advanced the differentiation of hPSCs for cell-based therapy in two different ways. Firstly, it shows that production of HLCs in GMP like condition is …
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Mapping Human Liver Development using Single-Cell Transcriptomics to Reveal Pathways Directing Stem Cell Fates
… formation and vascularisation in support of hepatoblast growth and differentiation during development. We utilised this information to derive bipotential hepatoblast organoids and used this novel model system to validate the importance of key signalling pathways and developmental cues. …
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Engineered 3D microenvironments to understand and direct liver progenitor and cancer cell fate
The cellular microenvironment encompasses every chemical and physical factor surrounding a cell, including all other cells, cell-cell and cell-extracellular matrix (ECM) signaling interactions and any detectible change in conditions. As the interplay between every component of the microenvironment …
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Dissecting combinatorial microenvironmental regulation of cell fate and function using a multi-modal arraying platform
Engineered systems provide a means of deconstructing the microenvironmental cues which guide cell fate and function. These cues can include biochemical elements, as in ligand–receptor binding for cell–cell or cell–extracellular matrix (ECM) interactions, or responses to biophysical parameters, as …