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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Heparan Sulfate Proteoglycan"”.
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The Heparan Sulfate Proteoglycan Perlecan Regulates Axonal and Synaptic Stability
Heparan sulfate proteoglycans (HSPGs) form essential components of the extracellular matrix (ECM) and basement membrane (BM) and have both structural and signaling roles. Perlecan is a secreted ECM-localized HSPG that contributes to tissue integrity and cell-cell communication. In this thesis, I …
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Characterization of heparan sulfate proteoglycan receptor from a permanent rat liver cell line
… was undertaken to demonstrate the existence of a heparan sulfate proteoglycan (HSPG) specific receptor on the cell surface of rat liver cell (RLC), and to characterize the specificity of the ligand-receptor interaction, the properties of the receptor, and the importance of the receptor in HSPG …
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Fluorous assisted one-pot oligosaccharide synthesis and chemical synthesis of homogeneous heparan sulfate proteoglycan
… are synthesized using this method. Hpearan sulfate proteoglycans (HSPGs), including syndecans and glypicans, are ubiquitous components of mammalian cell surface and extracellular matrix and they play important roles in various biological processes such as cancer development, angiogenesis. …
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Correlation of Heparan Sulfate Proteoglycan Structure and Metabolism With the Regulation of Cell Division
Heparan sulfate proteoglycan (HSPG), a ubiquitous cell surface component in animal cells, is a complex, polyanionic macromolecule consisting of a core protein to which are attached several heparan sulfate (HS) chains. Growing and confluent cultures of a rat hepatocyte cell line were labeled with …
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Metabolism of Heparan Sulfate Proteoglycan in Rat Hepatocyte Cell Line and Its Role for Regulation of Cell Growth
A rat hepatocyte cell line which accumulates free heparan sulfate (HS) chains in the nucleus was labeled with $\sp{35}$SO$\sb4\sp{2-}$ and the rate of appearance of ($\sp{35}$SO$\sb4$) HS in the nucleus was measured. ($\sp{35}$SO$\sb4$) HS began to accumulate in the nucleus 2 h after the addition …
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Identification, molecular cloning, and characterization of cerebroglycan, a cell surface heparan sulfate proteoglycan of the developing rat brain
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.
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Investigations of Insulin-Like Growth Factor I Cell Surface Binding: Regulation by Insulin-Like Growth Factor Binding Protein-3 and Heparan Sulfate Proteoglycan
… binding protein-3 (IGFBP-3), is influenced by heparan sulfate proteoglycans (HSPG). Initial research will be presented, assumptions and hypotheses that were included in the development of mathematical models will be discussed, and the future enhancements of the models will be explored. There …
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Drosophila Glypicans Regulate Follicle Stem Cell Maintenance and Niche Competition
… Here, it is demonstrated that two members of the heparan sulfate proteoglycan proteins in Drosophila, Dally and Dally-like (Dlp), differentially regulate follicle stem cell (FSC) maintenance and FSC competitiveness for niche occupancy. We find that dally is essential for normal FSC maintenance …
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The glypican Dally binds to Lipophorin particles and increases Hedgehog signaling efficiency
… is unknown. Here, I show that the gpi-linked heparan sulfate proteoglycan Dally is released from cell membranes and binds to lipoprotein particles both with and without its lipid anchor. Hedgehog signaling efficiency is reduced in Dally mutant discs, but can be rescued non-autonomously by …
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The role of heparan sulfate proteoglycans and heparanase in the control of vascular remodeling
… atherosclerosis and restenosis. We examined heparan sulfate proteoglycan homeostasis as a mechanism of regulation of arterial vascular remodeling in response to altered mechanical environments such as hypertension and injury. We first studied the effect of in-vitro mechanical strain on the …
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Tissue-Specific Matrix Control of Cell Cohesion and Migration Signaling Complexes
… constituents: collagens, glycoproteins, and proteoglycans, along with their enzyme modifiers. Within this network and beyond the structural role, each ECM molecule contributes a context-specific signal that influences cellular fate and behavior. Among these behaviors, cellular migration …
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Heparan Sulfate Proteoglycans in Pancreatic Ductal Adenocarcinoma (PDAC)
<p>We have previously identified a heparan sulfate proteoglycan (HSPG), Syndecan-1 (SDC1), as a key effector for pancreatic ductal adenocarcinoma (PDAC) progression whose surface expression is driven by KRAS*. By leveraging various PDAC models, we found that surface SDC1 expression was initially …
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A Novel Syndecan-1 Targeted Therapeutic Antibody Inhibits Macropinocytosis and Induces Anti-Tumor Immunity in Pancreatic Cancer
… identified Syndecan-1 (SDC1), a highly expressed heparan sulfate proteoglycan, as a critical downstream effector protein of KRAS*, which promotes cancer progression by mediating macropinocytosis. Moreover, SDC1 plays an essential role for the acquired resistance to KRAS* inhibition in PDAC, making …
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Extracellular matrix based substrates for propagation of human pluripotent stem cells
… the ECM substrates suggest that a combination of heparan sulfate proteoglycan and fibronectin was sufficient for the promoting hPSC sef-renewal. Finally, an in-direct co-culture system utilizing microporous membranes coated with acellular substrates and a physically separated feeder layer was …
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Vascular endothelial cell-surface proteoglycans
A predominant species of heparan sulfate proteoglycan that consisted of at least two subunits linked by disulfide bonding was isolated from cell layers of normal ("cobblestone") bovine vascular endothelial cells in culture. Treatment of the parent molecules with dithiothreitol caused their complete …
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Analysis of Cellular Factors Involved in Adeno-Associated Virus Type 2 Entry
… of sugar modified membrane protein known as heparan sulfate proteoglycans, have been shown to be a critical determinant in the efficiency of infection of many important pathogens. Adeno-associated virus type 2 (AAV) is a small (25nm) single stranded DNA, non-enveloped parvovirus that is …
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Systems biology of endothelial mechano-activated pathways
… was then assessed. Interestingly, we found that heparan sulfate expression is higher and evenly distributed on the apical surface of endothelial cells exposed to the atheroprotective waveform, and is irregularly present in cells exposed to the atheroprone waveform. Furthermore, the expression of …
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The prevention of heparanase expression in endothelial cells injured by high glucose
… or dysfunction, however, the degradation of heparan sulfate proteoglycan (HSPG) on the cell surface and in the extra cellular matrix (ECM) is considered to be of importance. Heparanase is believed to degrade heparan sulfate (HS). Our objectives were to determine if heparanase is responsible …
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Molecular basis oh herpes simplex virus entry into the cell and retargeting of the viral tropism for the design of oncolytic herpesviruses
… B (gB) and glycoprotein C (gC) interact with heparan sulfate proteoglycan to enable initial attachment to cell surfaces. In the next step, in the entry cascade, gD binds a specific surface receptor such as nectin1 or HVEM. The interaction of glycoprotein D with the receptor alters the …
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Characterization of the 37-kDa/67-kDa laminin receptor as the cell surface receptor for the cellular prion protein
… and cell-binding assays identified direct and heparan sulfate proteoglycan (HSPG)-dependent interaction sites mediating the binding of cellular PrP to the 37-kDa/67-kDa LRP/LR. The relationship between the 37-kDa LRP and the 67-kDa high-affinity LR is unknown so far. Both forms were observed in …
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