Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"HepaRG."”.
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Flaxseed Lignan Metabolites Modulate Hepatocellular Cholesterol Trafficking In HepaRG
… using fluorescence microscopy in the HepaRG cell line as an in vitro liver model. Specific dye markers for endoplasmic reticulum was used to identify the intracellular location of cholesterol trafficking and accumulation. Furthermore, the INSIG-SREBP cholesterol regulation pathway was …
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Farnesol-Mediated Regulation Of Hepatic Lipid Metabolism In Heparg Cells
… was created by incubating the hepatocyte-like HepaRG cell line with oleic acid. Farnesol treatment suppressed the OA-induced TGs accumulation in HepaRG cells by 25%. Farnesol upregulated the activity of lipid-sensing nuclear receptors PPARα and constitutive androstane receptor (CAR), which was …
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Investigation of Microbiome Metabolites and Mitochondrial Function in a model of Non-Alcoholic Fatty Liver Disease
… steatosis. Proliferative and differentiated HepaRG cells were cultured under standard conditions, and steatosis was established by 48h exposure to oleate and palmitate (2:1 molar ratio). Lipid accumulation was assessed by BODIPY™ staining and quantified by CellProfiler software. To gain …
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DEVELOPMENT OF IN VITRO NON-ALCOHOLIC STEATOHEPATITIS (NASH) MODEL FOR DRUG TESTING AND PATHOGENESIS STUDIES
… ballooning model is developed using HepaRG cells subjected to free fatty acids and Kupffer cell condition media to induce NASH-like phenotype. The induced ballooned hepatocytes exhibited typical features observed in in vivo settings. We recognized cytokeratin 18 fragment (M30) as a …
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Compartmental Hepatitis B Virus (HBV) Evolution, Replication and Infectivity in vivo and ex vivo
… in PBMC and exhibited infectious capacity to HepaRG cells in vitro. In summary, our data suggests potent NAs have little effect on HBV cccDNA in liver and PBMC, which highlights the need for continued suppressive antiviral therapy. The HBV evolution varied between different compartments in CHB …
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Investigating the Heterogeneity of Tumor Associated Macrophages in Human Hepatocellular Carcinoma
… macrophages. We used three HCC cell lines (Huh7, HepaRG, and HepG2) that broadly reflected the heterogeneous nature of HCC and established a co-culture system with primary monocyte-derived macrophages (MoDM). Co-culture with all HCC lines resulted in macrophages that exhibited an HCC-like …
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Development of 3D multicellular liver organoids derived from human induced pluripotent stem cells to model antiretroviral therapy induced liver injury
… high CYP3A4 enzyme activity compared to HepaRG 3D model. Proinflammatory (IL-6, IL-1β and TNF-α) and anti-inflammatory (IL-4 and IL-10) cytokines were elevated in both models. IL-4 was more pronounced in SEA-treated HLOs only. Proteomic analysis successfully showed differentially …
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Surface functionalized iron oxide nanoparticles for applications in biomedical sciences
… applications. In Chapter 3, the response of HepaRG liver cells to nanoparticles is examined in two methods, 2D and 3D cell culturing. By analyzing the cell response in 2D and 3D cultures an accurate estimation of the toxicity of nanoparticles can be made. The toxicity of iron oxide was …
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Identifizierung und Charakterisierung viraler und zellulärer Komponenten, die an den frühen Schritten der HBV-Infektion beteiligt sind
… dass die differenzierbare Hepatomzelllinie HepaRG mit HBV infiziert werden kann (Gripon et al., 2002), ermöglicht es, den Aufnahmemechanismus von HBV zu untersuchen. In Infektionskompetitionsversuchen können nun virale und zelluläre Proteine auf ihre Beteiligung an den frühen Schritten der …
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Métabolisme des acides gras polyinsaturés dans l'inflammation et la prolifération cellulaire
… l'activité inhibitrice dans les cellules HepaRG. Ces résultats suggèrent que le dérivé cétone d'acide sinapique est un bon candidat pour des études futures in vivo. Ce travail aide à mieux comprendre l'effet de différentes doses d'AGPI sur la distribution lipidique. Il faut aussi souligner …
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Toward improved in vitro models for human health risk assessment : mechanisms of short-chain per- and polyfluoroalkyl substances (PFAS) toxicity.
The global prevalence of manufactured chemicals lacking comprehensive toxicological profiles poses a significant challenge. This issue is exemplified by the widespread contamination of per- and polyfluoroalkyl substances (PFAS) worldwide. Despite their extensive use in various consumer products, …