Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 73 for “"HepG2 cells"”.
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Epigenetic toxicity of PFOA in HepG2 cells and its role in lipid metabolism
… damage. Liver hepatocellular carcinoma cell line HepG2 was used as a model to study the PFOA induced inflammation in vitro on the cellular, epigenetic and metabolic levels. HepG2 cells were treated with 20 μM, 100 μM, 200 μM, 400 μM PFOA dissolved in DMSO for 48 hours before harvested for DNA, RNA …
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In vitro evaluation of the cytotoxic potential of Brickellia cavanillesi (Asteraceae) using HepG2 cells
… of B. cavanillesi. Hepatocellular carcinoma cells (HepG2) were submitted to both a dose- (IC50) and a time-dependent toxicity evaluation in the presence or absence of Fetal Bovine Serum (FBS). Herbal extracts (1-35%) were used for the IC50 study. In the time course study, cells were exposed …
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Proteomics of Oxidative Stress Using Inducible CYP2E1 Expressing HepG2 Cells and 3T3-L1 Adipocytes as Model Systems
… adipocytes and an inducible CYP2E1 expressing HepG2 cells. Enhanced oxidative stress in hypertrophic adipocytes is associated with metabolic dysregulation and insulin resistance. Because mitochondria generate reactive oxygen species (ROS), we monitored changes to the adipocyte mitochondrial …
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Molecular and cellular insights into iron regulation
… ovary-transferrin receptor variant (CHO TRVb1) cells resulted in increased hepcidin peptide secretion after 30 min and 2 hours (p<0.03) as well as 24 and 48 hours (p<0.01). Also, partial characterisation of the previously unknown CHO-gene sequences of Hfe, Slc40-a1 and Irp2, was achieved. To …
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Transcriptional regulation of basal and phenobarbital-mediated expression of rat cytochrome 2B genes
… rat CYP2B genes were analyzed by transfection in HepG2 cells and by binding of nuclear proteins. Deletion of sequences from $-$110 to $-$57 markedly decreased the transcriptional activity, suggesting the presence of strong cis-acting elements in this region. Sequences similar to a basal …
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BIOCHEMICAL ACTIONS OF A NOVEL CIS-TERPENONE
… that trans-terpenones can be metabolized in cells to form a cascade of metabolite products, which may be biologically active due to the generation of quinine and quinone methide reactive intermediates. Therefore, 3-hydroxy-cis-terpenone (HCT) and analogs were designed and synthesized in our …
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Towards Combined Computational and Experimental Studies on Toxicity of Silver Nanoparticles
… Toxicity of 10 nm citrate stabilized AgNPs on HepG2 cells were investigated. Experimental results show that the 10 nm citrate stabilized AgNPs begin to be toxic to HepG2 cells at a dosage that exceeds 1 ppm and LD50 was observed at 3 ppm. Elevated reactive oxygen species levels were seen upon …
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Protective effect of TM-25659, known as TAZ activator, on high-fat diet-induced insulin resistance in C57BL/6J mice
… resistance and inflammation in skeletal muscle cells 17 B. TM-25659 increased FGF21 levels by GCN2 pathways in skeletal muscle cells 22 C. Effects of TM-25659 reduced insulin resistance and inflammation in skeletal muscle cells treated with or without FGF21 siRNA 27 D. TM-25659 prevented HF-diet …
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Broccoli Components as Antioxidants: The Role of Isothiocyanates as Redox Modifiers via Glutathiolation
… of the biomarker dichlorofluorescein, DCFH, in HepG2 cells) using hydrophilic and lipophilic extracts of broccoli. For lipophilic extracts, ORAC correlated with inhibition of cellular oxidation of DCFH (r = 0.596, p = 0.006). Also, DNA damage inhibition by lipophilic extract was negatively …
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Mammalian cell cultures as a model system for the determination of cytotoxicity induced by cholesterol oxidation products
… origin. Oxysterols are known to be cyctotoxic to cells in culture and mode of toxicity has been identified as apoptosis in certain cell lines. The cytotoxicity of the oxysterols 25-hydroxycholesterol (25-OH) and 7β-hydroxycholesterol (7β-OH) was examined in two human cell lines, HepG2, a hepatoma …
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Relationship between Hepatitis B Virus Core Particles and Intracellular Motility
… established in hepatoma cell lines, HuH7 and HepG2 cells. Using Northern blot and Southern blot analysis, HBV transcription and replication were detected in HBV replicating stable cell lines. Distribution of intracellular organelles was elucidated by immunfluorescence staining. Mitochondria …
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Antioxidant Potential in Broccoli and Observations on Flavor in a Segregating Broccoli Population (Brassica Oleracea)
… the antioxidant effects of broccoli extracts in HepG2 cells. The relationship between content of glucosinolates and SMCSO was also characterized in a broccoli population. ORAL assay findings indicated that water fractions of broccoli extracts had higher antioxidant capacity than lipid fractions …
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Effects of combined exposure to ethanol and ionizing radiation on the antioxidant status of in vitro and in vivo models
… in vitro model used in this investigation was HepG2 cells (human hepatocellular liver carcinoma cell line), while the in vivo model was Cd-1 mice"--Abstract, page iii.
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Design and synthesis of aryl hydrocarbon receptor fusion proteins for polyclonal antibodies production and cellular delivery
… order to deliver the CΔ553 into mammalian cells, an expression vector was constructed to generate the TAT-CΔ553 fusion protein. The TAT-CΔ553 fusion protein was successfully transduced into two mammalian cells-HeLa and HepG2. The in vivo function of TAT-CΔ553 was …
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Transcription regulation of the class II alcohol dehydrogenase 7 (ADH7)
… enhancer blocking activity in liver-derived HepG2 cells. This enhancer blocking function was cell- and position- dependent with no activity seen in CP-A esophageal cells. iA1C had a similar effect on the ectopic SV40 enhancer. The CCCTC-binding factor (CTCF) bound iA1C in HepG2 cells but not …
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SCREENING AND IDENTIFICATION OF NEW FOOD DERIVED PEPTIDES WITH MULTI-TARGET CHOLESTEROL-LOWERING ACTIVITY
… by differentiated human intestinal Caco-2 cells through transcytosis, and, during transport, it was partially metabolized in a breakdown fragment (LPKHSDAD, P5-met), which retained the biological activity of the parent peptide. Based on these observations, P5 analogs, including P5-Best …
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Regulation of Highly Unsaturated Fatty Acid Metabolism
… mature SREBP-1 and D6D mRNA in rat liver and in HepG2 cells. D6D promoter-luciferase assay in HepG2 cells demonstrated that sterol regulatory element is required for suppression of D6D transcription by ethanolamine and eritadenine. These results suggest that SREBP-1 activation may be regulated by …
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Investigating the role of UDP-<i>N</i>-acetylhexosamine pyrophosphorylases in regulating <i>O</i>-GlcNAcylation in human disease
… has been shown to provide beneficial effects on cells in coping with environmental stresses, such as hypoxia and glucose deprivation, and on the development of many cancers where O-GlcNAc levels are often up-regulated.<br/><br/>The action of OGT relies on the steady supply of UDP-GlcNAc, the end …
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Modulators of Inflammation and Steroid Receptors Regulate Proteinase Inhibitor 9 Gene Expression
… of p65. Dexamethasone induced IkappaBalpha in HepG2 cells. This may be one of the mechanisms used by Dex-GR to interfere with NF-kappaB activity and block IL-1beta-induced PI-9 gene expression. Our findings show that liganded GR may regulate IL-1beta induction of PI-9 expression through …
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