Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 166 for “"HepG2"”.
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RNA interference targeting Glucose-Regulated-Protein 78 induces HepG2 cell Apoptosis
… a cell culture model of human liver cancer, the HepG2 cell. The siRNA-hyper-branched A-1-2 construct produced 18% more knockdown when compared to its linear siRNA controls (siRNA-GRP78-1 + GRP78-2). This result highlights the synergistic effects of the novel A-1-2 construct, which combines two …
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Epigenetic toxicity of PFOA in HepG2 cells and its role in lipid metabolism
… damage. Liver hepatocellular carcinoma cell line HepG2 was used as a model to study the PFOA induced inflammation in vitro on the cellular, epigenetic and metabolic levels. HepG2 cells were treated with 20 μM, 100 μM, 200 μM, 400 μM PFOA dissolved in DMSO for 48 hours before harvested for DNA, RNA …
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In vitro evaluation of the cytotoxic potential of Brickellia cavanillesi (Asteraceae) using HepG2 cells
… B. cavanillesi. Hepatocellular carcinoma cells (HepG2) were submitted to both a dose- (IC50) and a time-dependent toxicity evaluation in the presence or absence of Fetal Bovine Serum (FBS). Herbal extracts (1-35%) were used for the IC50 study. In the time course study, cells were exposed to 1-10% …
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Regulation of Phospholipid Transfer Protein Secretion and Gene Expression in the Hepatoma Cell Line, HepG2
Hep G2 cells were used as a model to study HDL distribution and interconversion corresponding to variations in PLTP activity. An increase in PLTP activity by butyrate treatment was accompanied by increases in the amount of HDL having a hydrated density of 1.10 g/ml, HDL particle size and the …
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Proteomics of Oxidative Stress Using Inducible CYP2E1 Expressing HepG2 Cells and 3T3-L1 Adipocytes as Model Systems
… adipocytes and an inducible CYP2E1 expressing HepG2 cells. Enhanced oxidative stress in hypertrophic adipocytes is associated with metabolic dysregulation and insulin resistance. Because mitochondria generate reactive oxygen species (ROS), we monitored changes to the adipocyte mitochondrial …
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Über die Beteiligung EGF-Rezeptor-vermittelter Signaltransduktionswege an den tumorpromovierenden Effekten von 2-Acetylaminofluoren, 2-Nitrosofluoren und Phenobarbital in HepG2-Zellen
… C (PKC), der Protoonkogene c-FOS und c-JUN in HepG2 Zellen bestimmt. Nur PB hemmte die Expression des EGF-R, wohingegen die PKC, c-FOS und c-JUN nicht beeinflusst wurden. 2-AAF, 2-NOF und PB wirkten konzentrationsabhängig zytotoxisch und antiproliferativ auf HepG2-Zellen. Die PKC scheint an …
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Poly(Arginine) Derived Cancer-Targeting Peptides for the Development of a Cancer-Targeted Gene Therapy Approach in HepG2 Liver Cancer Cells
… relationships were next assessed in HepG2 hepatoblastoma cells, in which the cancer-targeting peptides were found to bind to the GRP78 receptor and internalize within the HepG2 cells. This discovery has led to their applications in cancer-targeted gene therapy, from which a …
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Development of exosome-based anti-cancer therapeutics
… of exosomes from human liver cancer cell line HepG2 (HepG2-Exo) under different conditioned media with modified FBS to deliver the best approach for exosome production. Thus, two media were developed for growing HepG2 cell line which are M1 and M2 using dulbecco's modified eagle medium (DMEM), …
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Molekulare Mechanismen des Tumorzelltodes induziert durch die Suizidgene Thymidinkinase und Purinnukleosidphosphorylase
… wurden humane HCC-Zellen der Zellinien HepG2 und Hep3B mit PNP oder TK über adenovirale Vektoren transduziert und anschließend mit der jeweiligen Prodrug inkubiert. Beide Systeme induzierten überwiegend Apoptose in HepG2- und Hep3B-Zellen. PNP/Fludarabin induzierte eine starke …
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Molecular and cellular insights into iron regulation
… recombinant transferrin receptor 1 (rec-TfR1) HepG2 cells were created which express modified TfR1 to maximise iron uptake. Upon holotransferrin (5 g/L) treatment these cells showed significantly increased iron uptake which was in contrast to the response by Wt HepG2 cells. Also, it was shown …
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Kationische Copolymere für den rezeptorvermittelten Gentransfer
… an HeLa-Zellen), Triiod-L-thyronin (HepG2-Zellen) und die Uronsäuren der Galactose, Mannose, Glucose sowie die Lactobionsäure (HeLa-, HepG2- und 16HBE-Zellen) verwendet. Das PEI, die Pfropfcopolymere PEI-g-PEO und die Ligand-funktionalisierten Copolymere wurden hinsichtlich ihrer …
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Modulation der Insulinsignalgebung durch Prostaglandin E2 und Endocannabinoide
… untersucht werden. Es wurden HepG2-Zellen, die keinen funktionalen EP-Rezeptor aufwiesen, sowie HepG2-Zellen, die stabil den EP1-R (HepG2-EP1), den EP3β-R (HepG2 EP3β) oder den EP4-R (HepG2 EP4) exprimierten, sowie die humane fötale Hepatozytenzelllinie, Fh hTert, die den EP2- …
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Anti-hyperlipidemic activities of Ilex latifolia and other selected herbs
… expression, and on LDL receptor expression in HepG2 and AML-12 hepatocytes. Ilex latifolia effect on mitochondrial metabolism, and glucose uptake was assayed in HepG2 and AML-12 hepatocytes. Chapter I is an introduction to hyperlipidemia, its types, causes, and treatment. A review of the …
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Mammalian cell cultures as a model system for the determination of cytotoxicity induced by cholesterol oxidation products
… (7β-OH) was examined in two human cell lines, HepG2, a hepatoma cell line, and U937, a monocytic cell line. Both 25-OH and 7β-OH were cytotoxic to the HepG2 cell line but apoptotic cells were not detected and it was concluded that cells underwent necrosis. 25-OH was not cytotoxic to the U937 …
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Transcriptional regulatory elements for basal expression and effects of phenobarbital and dexamethasone onmRNA levels of rabbit CYP2C genes
… gene and analyzed for promoter activity in HepG2 (hepatic) and COS-1 (non-hepatic) cells. Maximum basal expression in HepG2 and COS-1 cells was observed when the constructions contained C1 and C2 gene fragments that extended to $-$2000 and $-$410 bp (base pairs), respectively.
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UNVEILING THE ADVERSE EFFECTS OF POLYSTYRENE NANOPLASTICS IN THE HUMAN-DERIVED HEPG2-OA STEATOTIC-LIKE MODEL, VALIDATING DYSFUNCTIONAL HUMAN-DERIVED SW872 ADIPOCYTE IN VITRO MODELS FOR THE STUDY OF CARDIOMETABOLIC DISEASES, AND ASSESSING TRIMETHYLAMINE N-OXIDE IN A PLIC STUDY SUB-COHORT
… linea cellulare di epatoma derivata dall'uomo, HepG2, e in un modello di cellule HepG2 steatosiche indotte da 100 μM di acido oleico per 7 giorni (cellule HepG2-OA). Gli effetti citotossici indotti dalle PSNP sono stati valutati con il test di vitalità MTT. L'assorbimento delle PSNP e …
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Transcriptional regulation of basal and phenobarbital-mediated expression of rat cytochrome 2B genes
… rat CYP2B genes were analyzed by transfection in HepG2 cells and by binding of nuclear proteins. Deletion of sequences from $-$110 to $-$57 markedly decreased the transcriptional activity, suggesting the presence of strong cis-acting elements in this region. Sequences similar to a basal …
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BIOCHEMICAL ACTIONS OF A NOVEL CIS-TERPENONE
… effects of cis-terpenones on the viability of HepG2 cells exposed to aflatoxin B1 (AFB1). Cis-terpenones combined with AFB1 in HepG2 cells increased cell viability suggesting chemopreventive effects against AFB1. Further study revealed a mechanism for this effect: HCT and oxidized HCT (OHCT) …
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