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Showing 1 to 20 of 44 for “"Hemolysin"”.
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Treponema hyodysenteriae: growth and production of hemolysin
… (or cholestanol) for growth, and it produces a a-hemolysin (TH) which is induced (300 fold) by the addition of RNA core to cultures. TH is bound to the RNA core which acts as a carrier or stabilizer. I\lyobjectives were to (i) obtain successful continuous growth of T. hyodysenteriae; (ii) study …
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Analysis of Polyethylene Glycol in the α-Hemolysin Nanopore
… molecules of interest. These pores are usually α-hemolysin which is a toxin from Staphylococcus aureus or more recently nanoscale synthetic solid state pores. Now we are beginning to look at other molecules or proteins by sending them into the nanopores and measuring a characteristic partial …
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Avian Erythrocyte Receptor Substance for the Natural Hemolysin in Rat Serum
<p>[Abstract not included]</p> <p>[Signature page not included]</p>
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Catalase, superoxide dismutase, and hemolysin in the virulence of Listeria monocytogenes
Six strains of Listeria monocytogenes were examined for catalase, superoxide dismutase (SOD), and listeriolysin O (LLO) activities. The two strains having the lowest catalase activities also possessed the lowest SOD activities. Exogenous hydrogen peroxide was added to suspensions of four L. …
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Production and application of antibodies produced against the flagella and hemolysin of Vibrio parahaemolyticus
… of oyster homogenate. In the second method anti-hemolysin serum was produced and employed in Direct colony immunoblot (DCI) protocol to detect pathogenic Vibrio parahaemolyticus. DCI when compared with the currently used conventional method, DNA hybridization (DNAH) showed good correlation and …
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Analysis of a Tn917 Transposon Mutant and Preliminary Characterization of NonHemolytic, Catalase-Deficient Variants of Staphylococcus aureus
… isolated and analyzed due to its deficiency in hemolysin and lipase activities. The transposon insertion did not occur in any of the known genetic regulators, which suggested the insertion occurred in a novel regulator of at least, hemolysin and lipase activities. One end of the region where the …
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Nanopore Detector Feedback Control Using Cheminformatics Methods Integrated with Labview/Labwindows Tools
… or polypeptide) can be examined using an alpha-hemolysin channel detector. When a biopolymer is present in an alpha-hemolysin channel it can produce a highly structured ionic current blockade pattern, where the lifetimes at various sub-blockade levels reveal information about the kinetics of the …
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Understanding gene expression with a pore forming toxin
… pore forming toxin (staphylococcal α-hemolysin (αHL)) and to develop a new biotech application using the same protein.
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Purification, serology and pathogenic role of the 110 kilodalton rtx hemolysins of Actinobacillus pleuropneumoniae
… properties of the 110-kilodalton (110-KDa) hemolysins [hemolysin I (HlyI) and hemolysin II (HlyII)] of <i>A. pleuropneumoniae</i>. Several monoclonal antibodies (MAb) to the hemolysins were developed. An IgGl. MAb (8C2) specific for HlyII, as determined by immunoblotting, was cross-linked to …
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Symbiotic Associations and Pathogenic Potential of Vibrios In the North Inlet Estuary
… for the species specific marker thermolabile hemolysin (tlh) and the human virulence marker genes thermostable direct hemolysin (tdh) and thermostable direct hemolysin-related hemolysin (trh). Newly designed primers were employed for PCR reactions targeting tdh and trh which were found to be …
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Analysis of Nanopore Detector Measurements using Machine Learning Methods, with Application to Single-Molecule Kinetics
… The voltage draws a DNA molecule into an á-hemolysin channel in the membrane. Consequently, a distinctive channel current blockade signal is created as the molecule flexes and interacts with the channel. This flexing of the molecule is characterized by different blockade levels in the …
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Selective molecular transport in nanopore systems
… probe capture in bacterial toxin protein α-hemolysin (aHL) using dielectrophoresis. Third, I introduce the first simulation of molecular artificial water channel pillar[5]arene (PAP) and uncover the mechanics of its water transport and self-aggregation properties. Fourth, I characterize the …
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Intercellular communication in uropathogenic Escherichia coli and the probiotic Lactobacillus reuter
… motility or biofilm formation, but did alter α-hemolysin activity. L. reuteri RC-14 was shown to produce AI-2 in non-traditional expression patterns. Complementation of the E. coli J96 IuxS mutant with L. reuteri RC-14 luxS showed that the anaerobic, gram positive, luxS gene is functional in the …
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Power Signal Analysis of Channel Current Signal Using HMM-EM and Time Domain FSA
The Nanopore Detector using á-hemolysin channel transcribes kinetics of a single molecule along the nanometer-scale pore. The transcribed data is represented by electrical measurements. We present accurate and computationally inexpensive tools to analyze single molecule kinetics. The HMM-EM level …
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Isolation and Use of the Aeromonas hydrophila Secretin ExeD for Nanopore Analysis
… the size of the most commonly used nanopore, α-hemolysin. The narrow vestibule of the pore doesn’t allow proteins or peptide aggregates to pass through its channel. A larger pore, using the type II secretin of Aeromonas hydrophila, may be able to circumvent the size constraint of α-hemolysin and …
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CHARACTERIZATION OF INDIVIDUAL CHARGED Au25(SG)18 CLUSTERS AND THEIR ENHANCEMENT OF SINGLE MOLECULE MASS SPECTROMETRY
… glycol (PEG) molecules within an alpha hemolysin (αHL) nanopore. The effect appears over a range of PEG sizes and ionic strengths. This increases the resolution of the peaks in the single molecule mass spectrometry (SMMS) current blockade distribution and suggests a means for reducing …
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Nanoscale control in biological and synthetic systems
… translocation through the biological nanopore α-hemolysin. I have also employed MD simulations to explore methods for enhancing experimental control over the translocation of DNA through synthetic nanopores.
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Assessment of microbial quality and safety of ground beef/product sold in differrent retailers around Thohoyandou Area, Vhembe District, Limpopo
… different virulence genes and pathotypes. Hemolysin test was done in blood agar plates to identify virulence characteristics of E. coli isolates. Results: Out of 160 samples analyzed, E. coli was detected in 80 (50%), Staphylococcus spp. in 117 (73.12%), Salmonella in 60 (37.5%), and …
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