Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Heme Biosynthesis"”.
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Enhance heme biosynthesis in Saccharomyces cerevisiae
This research delves into the enhancement of heme production in Saccharomyces cerevisiae through Adaptive Laboratory Evolution (ALE) and Atmospheric and Room Temperature Plasma (ARTP) mutagenesis, complemented by strategic nutrient supplementation. Despite the theoretical promise of ALE and ARTP to …
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Characterization of the Hepatotoxicity of Rifampicin and Isoniazid
… of protoporphyrin ix (PPIX), the penultimate heme precursor. Excess PPIX is believed to bind to bile acids, precipitate in bile canaliculi, and form bile plugs leading to cholestasis fol owed by liver injury. Both ferrochelatase (<em>FECH</em>/<em>Fech</em>) and aminolevulinic acid synthase 1 …
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Staphylococcus aureus small colony variants use heme and staphyloferrin B for iron acquisition
… iron are unknown. Mutation of hemB, involved in heme biosynthesis, generated a stable SCV that was auxotrophic for hemin and formed small colonies on solid media. To support growth under iron deplete conditions, my data revealed that S. aureus hemB synthesizes the siderophore staphyloferrin B, …
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An Intermediate Heme Threshold is Necessary for the Activation of the Stress Response and Redox Homeostasis in Yeast
… we reveal that an intermediate threshold of heme biosynthesis is sufficient to activate the stress response and maintain mitochondrial redox homeostasis but not mitochondrial respiration. In the first result Chapter (Chapter 3), using the hem1∆ mutants supplemented with different …
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The development of a novel therapeutic strategy for the treatment of prostate cancer by targeting metabolic signalling
… in essential pathways including lipogenesis, heme-biosynthesis, redox homeostasis, and glycolysis. The lead targets were validated in a range of cell lines and additional assays performed to investigate the effect upon cell cycle and cell death. UROS, the fourth step of heme synthesis, was …
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Probing Iron Accumulation in Sacchromyces cerevisiae Using Integrative Biophysical and Biochemical Techniques
… iron sulfur (Fe/S) cluster assembly and heme biosynthesis. However it is also potentially toxic due to its ability to induce formation of reactive oxygen species (ROS) via Fenton chemistry. Therefore its uptake, trafficking and utilization must be regulated to avoid its toxicological …
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ABCB6 Is a Porphyrin Transporter with a Novel Trafficking Signal That Is Conserved in Other ABC Transporters
… as a porphyrin transporter that facilitates heme biosynthesis, in which the intermediates must be shuttled between mitochondria and the cytosol. Translocation of substrates by ABC transporters occurs in an ATP-dependent manner, although the role of ATP binding and hydrolysis in the transport …
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Investigating Subcellular Thiol Redox Chemistry With Gfp-Based Redox Sensors
… pathways such as respiration, Fe-S cluster and heme biosynthesis, and biosynthesis of lipids, amino acids and nucleotides. These pathways are all dependent on cysteine-containing proteins, thus maintaining thiol-disulfide balance in this organelle is critical for cellular function. …
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DIETARY INTERVENTIONS TARGETING GLUCOSE METABOLISM AND HYPERINSULINEMIA: A NEW TRANSLATIONAL PERSPECTIVE FOR THE MANAGEMENT OF ACUTE INTERMITTENT PORPHYRIA
… deaminase (PBGD) enzyme and reducing hepatic heme availability. In stressful conditions, as fasting, which stimulates the demand of heme production, the delayed heme biosynthesis leads to the accumulation of porphyrins ’precursors, involved in neurotoxicity, and hyperactivity of ALAS1, the …
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Utilizing in vivo genome-wide CRISPR/Cas9 screens in immunocompetent mouse models to identify novel tumor liabilities and explore mechanisms of resistance to Chimeric Antigen Receptor T-cell therapy
… Results from these screens highlighted the heme biosynthesis pathway as an in vivo specific dependency and potential novel therapeutic target. In addition, processes of cell motility and migration were implicated in promoting resistance to CAR-T therapy in GBM. The third screen conducted in …
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Investigating the molecular pathogenesis of tuberculosis-associated immune reconstitution inflammatory syndrome
… those who did not, included chondroitin sulfate biosynthesis and interleukin (IL)-6. These finding suggest that paradoxical TB-IRIS is preceded by increased expression of antimicrobial peptides and the impairment of immune cellular function controlled by the extracellular matrix. Pathway analysis …