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Showing 1 to 14 of 14 for “"Helix-helix"”.
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Elucidating Pseudomonas aeruginosa virulence gene regulation by the ribbon-helix-helix transcription factor AmrZ
… among others. AmrZ, a member of the ribbon-helix-helix family of DNA binding proteins, regulates several virulence factors, including alginate biosynthesis, flagellum expression, and type IV pili localization and twitching motility. AmrZ also acts as an autorepressor by binding two sites on …
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The Role of Transmembrane Domain Helix-Helix Interactions in the Function of Pentameric Ligand-Gated Ion Channels
… lipids. The lipid-exposed transmembrane alpha-helix, M4, in each homologous subunit likely acts as a lipid sensor. One model proposes that activating lipids promote M4 “binding” to the adjacent alpha-helices, M1 and M3, to enhance interactions between the M4 C-terminus and the Cys-loop of the …
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The development of stapled peptides as chemical tools to investigate activin A signalling and as antibacterial agents targeting MsbA-mediated efflux
… to investigate PPIs, and closely related helix-helix packing sites, relevant to human health. 1. The development of stapled peptides as chemical tools to investigate activin A signalling Activin A signalling has been implicated in a wide range of cancers as well as the orphan disease …
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Crystallographic studies of the metal-responsive transcription factor NikR
… NikR tetramer, consisting of two dimeric Ribbon-Helix-Helix (RHH) DNA-binding domains separated by a tetrameric regulatory domain that binds metal ions (MBD). NikR is the only known metal- responsive member of the RHH family of transcription factors, and the structural relationships between NikR …
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Characterization of an Amphipathic Alpha-Helix in the Membrane Targeting and Viral Genome Replication of Brome Mosaic Virus
… examined the possible role of an amphipathic α-helix, helix B, in BMV 1a's membrane association. Deletion or single substitution of multiple amino acids of helix B abolished BMV 1a's localization to nuclear ER membranes. Additional reporter-based, gain-of-function assays showed that helix B is …
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SPECIFIC RESIDUES WITHIN PSEUDOMONAS AERUGINOSA AmrZ ARE REQUIRED FOR TRANSCRIPTIONAL REGULATION OF VIRULENCE FACTORS CONTRIBUTING TO INFECTION
… strong advantage for survival. AmrZ is a ribbon-helix-helix transcriptional regulator that controls several virulence factors—alginate production, type IV pili expression and twitching motility, and flagellar expression. AmrZ acts as both an activator and a repressor, suggesting it works in a …
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The evolution and regulation of DNA-binding by the nickel-dependent transcription factor NikR
… factor NikR is a member of the ribbon-helix-helix: RHH) family of DNA-binding proteins and is widespread among bacteria and archea with vastly different nickel physiologies. The goal of this thesis was to better understand basic aspects of cellular transition metal homeostasis by …
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The Calcium Channel Gamma6 Subunit Analysis of Function and Determination of a Sequence Motif Critical for Its Effect
… to produce a long groove on one face of the helix due to the short side chains of the glycine and alanine residues. GxxxA and related motifs are thought to be important for promoting and stabilizing helix-helix interactions. Therefore we performed site directed mutagenesis of these specific …
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Computational and Experimental Investigation of Allosteric Communication in the Transcriptional Regulator NikR
… each chain contains both a DNA binding ribbon-helix-helix: RHH) domain and a Ni<super>2+</super> binding regulatory ACT: <underline>a</underline>spartokinase, <underline>c</underline>horismate mutase, <underline>T</underline>yrA) fold. Work herein combines computational modeling of NikR …
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Relating metal binding to DNA binding in the nickel regulatory protein NikR
… interface and two flanking dimeric ribbon-helix-helix (RHH) DNA-binding domains. Early work revealed that NikR can bind a variety of transition metal ions and has two binding affinities for the nik operon: nM when stoichiometric Ni2+ binds NikR and pM when excess Ni2+ binds. The enhanced …
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Computational investigations of cellular functions: Three cases on membrane morphogenesis, organization and assembly of a multi-protein complex, and the molecular origin of muscle elasticity
… states, and the residues participating in PufX helix-helix interactions in the dimeric state were identified. The dimerized PufX helices display a stable GlyA-like crossing angle, which, during the self-assembly process, possibly results in the highly bent and V-shaped structure of core complex …
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Novel Strategies towards the Inhibition of ATP-Binding Cassette (ABC) Transporters from Pathogenic Bacteria
… spanning synthetic peptides that disrupt helix-helix interactions required for conformational change. Three initial approaches were employed; (i) fragmentation of helices seen as cytotoxic in a preliminary study, (ii) generation of solubility-tagged membrane spanning sequences, and (iii) …
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Impact of formulation and processing conditions on the material properties and sensory characteristics of confectionary products and model systems across the stages of sugar cooking
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01
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Exploring the molecular architecture of proteins: Method developments in structure prediction and design
<p>Proteins are molecular machines of life in the truest sense. Being the expressors of genotype, proteins have been a focus in structural biology. Since the first characterization and structure determination of protein molecule more than half a century ago1, our understanding of protein structure …