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Showing 1 to 13 of 13 for “"Helix pomatia"”.
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Affinity purification of blood group A-active glycolipids on immobilized Helix pomatia lectin
… specificity of the lectins from Helix pomatia, Wisteria floribunda and Dolichos biflorus. All three lectins detected the Forssman glycolipid which has a terminal GalNAcα1-3 residue. The Helix pomatia and Wisteria floribunda lectins are also bound to glycolipids with GalNAcβ-linked …
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Identification of tumour-associated gycoproteins recognised by the lectin from Helix pomatia in breast cancer
Helix pomatia agglutinin (HPA) is a carbohydrate binding protein isolated from the Roman snail. There has been considerable interest in understanding HPA binding partners in cancer, as the lectin has been shown to identify glycosylation changes in cancers arising from the epithelia, from patients …
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Characterisation of the Helix pomatia agglutinin binding glycoproteins of colorectal cancer cell lines and tissue samples
… prognosis. The lectin from the Roman snail Helix pomatia (HPA) has attracted interest as tool for the detection of metastatic colorectal cancer but the HPA binding partners have remained poorly characterised. We established an in vitro model using human colorectal cancer cell lines ranging …
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Imaging studies of glycan mediated breast cancer epithelial cell-endothelial cell interactions
… (GalNAc), specifically recognized by the lectin Helix pomatia agglutinin (derived from the albumin gland of the Roman snail, Helix pomatia), have been described as a hallmark of aggressive cancers of the breast and several other sites. The research described in this thesis aims to study the role …
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Metastatic cell responses to anticancer therapy: The possible role of cancer stem cells
… such as Tn antigen which can be detected by Helix pomatia agglutinin (HPA) lectin extract from Helix pomatia snails. Radiotherapy could have an effect on cancer metastasis; by stimulating regulation signalling including ROS, TGF-β, E-cadherin and vimentin. This signalling can promote cancer …
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Profiling of urinary metabolites in human pregnancy
… enzymes from the digestive juice of the snail, Helix pomatia. Purified urinary organic acid and steroid extracts were methoximated and trimethylsilylated for gas-chromatographic analysis. Urinary acid profiles contained 50 recognizable peaks, 10 of which were unequivocally identified by mass …
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Purification of glycoproteins from herpes simplex virus
… chromatography using wheat germ lectin and Helix pomatia lectin was not successful in purifying the glycoproteins because the glycoproteins which bound to the lectins could not be eluted under normal conditions. Difficulties encountered in eluting the HSV-1 glycoproteins from the lectins may …
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Investigations into altered cellular glycosylation associated with metastatic competence in breast cancer cell lines
… mechanisms. HPA, a lectin from the Roman snail Helix pomatia, recognises glycans terminating in α-GalNAc, and is associated with aggressive biological behaviour, poor patient prognosis and metastasis in breast and other cancers. Three breast cancer cell lines characterised to stably synthesise …
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Glycolipids in mouse F9 teratocarcinoma cells: some changes associated with retinoic acid-induced differentiation
… chromatography on columns of immobilized Helix pomatia. Wisteria f1oribunda. Griffonia simplicifolia-I and Ricinus communis-' agglutinins followed by reduction and permethylation revealed that globoside (GaINAcβ1, 3Galα1, 4Galβ1, 4Glc) and lactose (Galβ1,4Glc) are the principal …
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Analysis of aqueous matrices using supercritical fluid extraction in conjunction with chromatographic spectroscopic and mass spectrometric techniques
… solution incubated with <i>Helix pomatia</i> glucuronidase. Other studies described within this chapter that also involved the use of direct aqueous supercritical fluid extraction with off-line gas chromatography-mass spectrometry describe procedures for determining trace …
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Diagnostic use of hair analysis for the detection of misuse of amfetamines and cannabinoids
… methods, alkaline (1M NaOH), β-Glucuronidase (helix pomatia), methanol (MeOH) and acid (0.1M HCI) were used on these and compared. The best recovery for amphetamine was obtained using the β-glucuronidase pre-treatment method and this extract was also found to be cleaner than the alkaline and …
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THE DOUBLE BOND IN Δ1-2 POSITION OF STEROIDS: A MATTER OF CONTROVERSY IN THE CONTROL OF ILLICIT TREATMENTS OF FARM ANIMALS
… of deconjugation with beta-glucuronidase from Helix pomatia was assessed, too. Only urine where prednisolone and prednisone were absent and cortisol was higher than 80 ng/mL were used. In this study a liquid-liquid extraction with a mixture of tert-Buthylmethylether:Ethylacetate (80:20) was …