Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 193 for “"Helicase"”.
-
Single molecule studies of helicase mechanisms
Bloom helicase (BLM) is a member of the RecQ helicase family, a group of 3’ to 5’ helicases which are homologs of the RecQ protein found in E. coli. Besides the canonical helicase function of unwinding duplex DNA, the RecQ family of helicases is known to preferentially bind to non-canonical DNA …
-
XPD Helicase: Shifting the Inchworm Into Reverse
Directional translocation by helicases results in duplex separation and displacement of bound proteins which allows for the DNA processing events associated with DNA repair, replication, recombination, and transcription. Unresolved questions regarding DNA helicases include: (1) how is directional …
-
Single-molecule studies of eukaryotic helicase loading
… is the loading of the heterohexameric Mcm2-7 helicase. In eukaryotes helicase loading is tightly regulated to ensure that this event only occurs during the GI phase of the cell cycle. Because helicase activation can only occur once cells enter S phase, cells can only load and activate the …
-
Investigation of Cdc45 function during helicase activation
… requires the stepwise loading of Mcm2-7 helicase complexes at origins of replication. These loaded helicases need to be activated during DNA replication initiation to unwind the DNA double helix and provide a ssDNA template for replicative DNA polymerases. Eukaryotic cells segregate …
-
An ORC Flip Enables Bidirectional Helicase Loading
… by loading two inactive Mcm2-7 replicative DNA helicases around the origin DNA in a head-to-head double hexamer. This conformation prepares the helicases to initiate bidirectional replication upon entry into S-phase. Interactions between three proteins and the Mcm2-7 helicase guide eukaryotic …
-
The Biochemical Characterization of Drosophila melanogaster RecQ4 Helicase
… a member of the conserved RecQ family of helicases, is involved in replication and associated with several clinical syndromes. Although biologically important, the biochemistry of RecQ4 has remained elusive. We have expressed and purified Drosophila melanogaster RecQ4 from a baculovirus …
-
Investigating the cellular localisation of DEAD box helicase DDX3
DDX3 is an RNA helicase that has been shown to have a range of nuclear and cytoplasmic functions, including transcription, translation, splicing and mRNA export. DDX3 has also been shown to play a role in innate immune signalling and tumorigenesis. DDX3 is targeted by multiple viruses: HIV, HCV , …
-
Helicase-Initiated Assembly of Macromolecular Machines Involved in DNA Replication
… physical model of the initial phase of the PriA helicase-directed formation of the primosome, a large protein-DNA complex, in E. coli, responsible for the restart of the DNA replication after the damage. The primosome, in its final state, comprises of as many as seven different proteins: PriA and …
-
Homologous Strand Exchange and DNA Helicase Activities in Plant Mitochondria
… protein-promoted strand exchange reactions. DNA helicases utilize the energy of ATP to separate the two parental DNA strands at the replicating fork or during recombinational strand exchange. Although higher plant chloroplast helicase activity has been reported, no such activity has heretofore …
-
Single-molecule study of the DNA helicase regulating genomic stability
A DNA helicase, PcrA is an essential protein in gram-positive bacteria. PcrA utilizes its helicase activity and translocation activity to regulate various cellular functions including plasmid replication and counteracting deleterious recombination. Using single molecule FRET technique and …
-
Towards Understanding Helicase and Chaperone Activities in the RNA Degradosome
… the endoribonuclease RNase E, the DEAD-box RNA helicase RhlB, the glycolytic enzyme enolase, and the phosphorolytic exoribonuclease PNPase. The degradosome has also been previously shown to associate with the RNA chaperone Hfq to form a small RNA guided machinery that targets defined …
-
Single-molecule studies of the mechanism of eukaryotic helicase activation
… two key events that center the replicative DNA helicase Mcm2-7: helicase loading and helicase activation. During G1 phase, two inactive Mcm2-7 are loaded unto origin DNA. Upon entry into S phase, the association of multiple factors will promote helicase activity. Although loaded helicases mark …
-
Functional dissection of the S. cerevisiae helicase activating factor Cdc45
… initiation requires two essential steps: helicase loading and activation. During the G1 to S transition, loaded Mcm2-7 helicases are activated by the S-CDK (S phase cyclin-dependent kinase)- and DDK (Dbf4-dependent kinase)- dependent recruitment of the helicase activating proteins Cdc4S …
-
Genetic and biochemical analysis of a conserved DEAD-box helicase motif
DEAD-box proteins are non-processive helicases that use ATP to power unwinding of short RNA duplexes in essential steps of RNA metabolism. Despite acting on a diverse range of RNA substrates, DEAD-box proteins share a helicase core defined by 13 highly conserved amino acid motifs. Here we use S. …
-
Biochemical and Structural Insights into VCP Unfoldase and WRN Helicase Complexes
… the biochemical and structural properties of WRN helicase in complex with DNA substrates. I describe multiple efforts toward structure determination of WRN-DNA assemblies and additional studies where I examined the mechanism of action for a clinical-stage small molecule WRN inhibitor. While I have …
-
CHARACTERIZATION OF THE FUNCTION OF THE DEAD-BOX RNA HELICASE DBP2
… DEAD-box proteins are the largest class of RNA helicases that play fundamental roles in RNA and RNP structure remodeling. However, the precise biological role of the vast majority of the ~ 40 members in this family has not been completely described. Therefore, my research focused on …
-
Investigating the role of germline RNA helicase-1 in Caenorhabditis elegans
… AT AUTHOR'S REQUEST.] The germline specific RNA helicase GLH-1, a constitutive P-granule component, and DCR-1, the riboendonuclease responsible for processing small RNAs in the miRNA pathway, have been shown to be interdependent in the C. elegans germline. The work described here is based on the …
-
The Role of RNA Helicase RHAU in Immunoglobulin Class Switch Recombination
… between the AID G4 binding domain and the RNA helicase associated with AU-rich element (RHAU) specific motif (RSM), which allows RHAU binding to G4 RNAs and subsequent unwinding of G4 RNA into single-stranded transcripts. We hypothesize that RHAU functions in CSR by binding G4 S transcripts and …
-
RNA helicase MOV10 is essential for gastrulation and normal CNS development
… demonstrated that FMRP associates with the RNA helicase Moloney Leukemia Virus 10 (Mov10) in brain and modulates its translational activity through the microRNA (miRNA) pathway. FMRP is critical for normal cognition and our findings hypothesize a role for Mov10 in brain function. Additionally, …
Page 1 of 10