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Showing 1 to 20 of 234 for “"HeLa cells"”.
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Analysis of Interphase Chromatin Motion in HeLa Cells
<p>Motion of particles under influencing forces may be observed under light microscopy techniques. Variations in mobility of particles may give relevant biophysical information. Automated high resolution single particle tracking techniques were used to characterize interphase chromatin mobility in …
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Anomalous diffusion of mRNA in the cytoplasm of HeLa cells
… messenger RNA molecules in the cytoplasm of HeLa cells. RNAs are imaged in live cells via total internal reflection (TIRF) microscopy. In order to visualize individual RNA molecules expressing the MYH9 gene, they were labeled via MS2 stem loops bound to coat proteins tagged with the …
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Turnover Rate of The Neuronal Connexin Cx36 In Hela Cells
… of connexins (Cx26 and Cx43) in tissue culture cells and whole organs. We utilized HaloTag technology to study the turnover rate of Cx36 in transiently transfected HeLa cells. The HaloTag protein forms irreversible covalent bonds with chloroalkane ligands, allowing pulse-chase experiments to be …
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Pulse electric field exposure effect on morphological properties of HeLa cells
… pulsed electric field (PEF) towards biological cells. Biological cells selected in this study are HeLa (cervical cancer) cells. There are two parts of the study, which was involving modeling methods and experimental setup. Modeling method used involves analytical (MATLAB) and numerical (CST®EMS) …
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Peroxidase Content of Hela Cells During Radiation Injury and Recovery Processes
… radiation on oxygenated water. Since living cells always contain and are usually bathed in high concentrations of water and appreciable concentrations of oxygen, the conditions for production of HgOg by radiation are frequently present. A question of interest (Adler, 1963) may be phrased as …
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Transcription of the adenovirus genome during productive infection of HeLa cells.
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 1972
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The Effect of Lithium on Saccharomyces cerevisiae, Candida albicans, and HeLa cells
… Saccharomyces cerevisiae, Candida albicans, and HeLa cells. In yeast, elevated lithium disrupts fermentation, leading to nutrient build-up and reduced ethanol production, showcasing its impact on cellular metabolism. In human HeLa cells, lithium offers therapeutic potentials at lower doses, …
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Endocytosis mechanism of poly- and lipoplexes in siRNA delivery in HeLa cells
… which nutrients, signals and pathogens enter the cells. This process consists of multiple pathways that lead the objects that were ingested by the cells to different intracellular destinations. The chemical environment encountered by the endocytosed objects also depends on the specific pathways. …
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THE REGULATION OF POLYAMINES AND THEIR ROLE IN THE PROLIFERATION OF HELA CELLS
… activity was stimulated in high-density HeLa cell cultures by dilution of or replacement of spent culture medium with fresh medium containing 10% (v/v) horse serum. After stimulation, ornithine decarboxylase activity reached a peak at 4-6 h. then rapidly declined to the low enzyme …
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Extracellular enzyme assays of Blastomyces dermatitidis and HeLa cells in combined and separate populations
Thesis (M.S.)--Michigan State University. Dept. of Microbiology and Public Health,
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Development of a 3d bioprinting system, characterisation of bioink and printing microspheroids of hela cells
In Malaysia, Industrialised Building System (IBS) has been recognised as a potential solution in improving the deliverables of construction projects. However, the acceptance of this modern technology is still low. Most of the construction players prefer the conventional construction method which …
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A quantitative characterisation of phospholipid composition and biosynthesis in HeLa cells and nuclei - a mass spectrometry study
Nuclei from Human cells have been shown to contain a pool of endonuclear phospholipidthat is distinct from the nuclear membrane. It has been suggested that this endonuclear phospholipid is involved in the regulation of nuclear processes such as transcription. This study utilised tandem electrospray …
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Regulation of mRNA expression through 3’ UTR sequences of IL6 gene in amino acid-deprived HeLa cells
… triggered by amino acid-deprivation of cancer cells activate STAT3 and NF-κB transcriptional factors subsequently to induce IL6 cytokines. Here, it was investigated whether 3’UTR of IL6 mRNA played a role in IL6 induction through cellular stress responses. In the studies with GFP-reporter …
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Suppressive effect of a nucleic acid-hydrolyzing recombinant antibody (3D8) on viral multiplication
… virus (VSV) that belongs to RNA viruses in HeLa cells. When the purified 3D8 scFv was directly imported into HeLa cells by an electroporation and the cells were infected with VSV (MOI=10), significant inhibition against VSV multiplication and VSV-induced cytopathic effect (apoptosis) was …
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Host-Parasite Interrelations: Bacterial Adherence to and Invasion of Epithelial Cells
… (EIEC) preferentially invaded multinucleated HeLa cells which arose naturally in monolayer cultures at a low frequency. Data suggests that enhancement of the penetration step accounts partly for increased invasion of multinucleated cells. Treatment of HeLa cell monolayers with polyethylene …
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Zein films as substrates for cell attachment
Circulating tumor cells (CTCs) are cells that have shed into the vasculature from a primary tumor and circulate in the blood stream. Circulating tumor cells play an important role in metastasis. The long term goal of this project is to capture and remocve CTC’s from the blood stream. Numerous …
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Effect of Combination of Aspirin and TRAIL in HeLa Cervical Cancer Cells
… of cytokines and induces apoptosis in most tumor cells. Although TRAIL is considered a promising drug for cancer therapies because of its tumor selectivity, many tumors are resistant to TRAIL. Thus TRAIL-resistant cancer cells must be sensitized first to become responsive to TRAIL. In this study, …
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Effect of disruption of 3D8 complementarity-determining regions on properties of 3D8 antibody
… Moreover, sc-H3i, sc-L3i and H-3i entered the HeLa cells and localized in cytosol of the HeLa cells like 3D8 scFv. It seems that at least H-CDR3 and L-CDR3 in 3D8 scFv format contribute to interaction with heparin and penetration the HeLa cells. For DNA-hydrolyzing activity, sc-L1i, sc-L3i, 3D8 …
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