Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Hansenula polymorpha"”.
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Online monitoring in continuously shaken microtiter plates for scalable upstream bioprocessing
… fermentations with Eschericha coli and the yeast Hansenula polymorpha as model organisms. It could be demonstrated that online biomass detection is possible up to biomass concentrations of 50 g/L cell dry weight due to a linear correlation between scattered light intensities and cell dry weight in …
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Integrierte Impedanzspektroskopie aerober Zellkulturen in biotechnologischen Hochdurchsatzscreenings
… culture used in this work is the yeast culture Hansenula Polymorpha. Even at low concentrations of less than 15 g/l the growth of these cells can be monitored clearly. In addition to this, tests with bacteria Escherichia Coli are carried out in complex TB-medium (terrific broth medium). As the …
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Nutzung eines 16-fach parallelen Bioreaktorsystems zur Entwicklung von Prozessen für die Herstellung von rekombinanten Proteinen in Mikroorganismen
… for the production of recombinant proteins in Hansenula polymorpha. Due to the improvement of the aeration system and the development of a robust fermentation strategy for parallel cultivation, the Hansenula-clone FMD-HSA 56-1 reached dry cell weights of up to 60 g/L and rHSA concentrations of …
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A new method to quantify the CO 2 sensitivity of micro-organisms in shaken bioreactors and scale up to stirred tank fermentors
… results on Arxula adeninivorans LS3 and Hansenula polymorpha (WT ATCC 34438 and RB11-FMD-GFP) indicated that the CO2 had no effect on these microorganisms. Pseudomonas fluorescens DSM 50090 on yeast extract + glucose and Corynebacterium glutamicum ATCC WT13032 on L-lactate were found to …
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Entwicklung und Anwendung einer Fed-batch-Betriebsweise mit Nährstofffreisetzungssystemen zur kontrollierten Kultivierung und zum Screening von Mikroorganismen in Schüttelreaktoren
… technique” was tested on the metabolism of H. polymorpha, E. coli and G. oxydans in shake flasks. The OTR and RQ were monitored online with a RAMOS device. Biomass formation, synthesis of proteins like GFP or eYFP-IL-6, pH drift and metabolic dynamics of glucose, ethanol, acetic acid and other …