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Showing 1 to 15 of 15 for “"Halogenase"”.
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Molekulargenetische und biochemische Untersuchungen zur Tryptophan-5-Halogenase aus der Biosynthese von Pyrroindomycin B in Streptomyces rugosporus
Regioselektive Halogenasen sind Enzyme, die für die Biosynthese verschiedener halogenierter Metaboliten verantwortlich sind. Der Stamm Streptomyces rugosporus produziert die bioaktive halogenierte Verbindung Pyrroindomycin B. In dieser Arbeit wurde ein Gen einer Tryptophan-5-Halogenase aus dem …
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Mechanisms of Enzyme Neofunctionalization in Plant Specialized Metabolism
… nonheme iron(II)- and 2-oxoglutarate-dependent halogenase and BAHD acyltransferase. First, we describe the genomic- and biochemical-basis for gene duplication and neofunctionalization of flavonol synthase (FLS) that led to the unique emergence of halogenase in Menispermaceae plants. The …
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Crystallographic studies on enzymatic halogenation of natural products
… solve structures of enzymes from two classes of halogenases, the flavin-dependent halogenases and the non-heme iron dependent halogenases. Structures of the flavin-dependent tryptophan 7-halogenase RebH from Lechevalieria aerocolonigenes, involved in rebeccamycin biosynthesis, were solved by …
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Two versatile cofactors, flavin adenine dinucleotide and non-heme iron, involved in DNA repair and natural product halogenation
… perform halogenation chemistry. Flavin-dependent halogenasess perform halogenation reactions on aromatic substrates, while non-heme iron-dependent halogenases perform halogenations reactions on unactivated aliphatic substrates. I is a flavin-dependent halogenase that catalyzes the chlorination of …
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Detektion und Überexpression von Genen FADH2- abhängiger Halogenasen aus Actinoplanes sp. ATCC 33002
… Biosynthese die Beteiligung FADH2-abhängiger Halogenasen vermutet wurde. Ziel dieser Arbeit war es deshalb, in Actinoplanes sp. nach Genen FADH2-abhängiger Halogenasen zu suchen und diese heterolog zu überexprimieren. Durch Hybridisierungsexperimente mit prnC[2] (Halogenasegen aus Pseudo¬monas …
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Untersuchungen auf Sekundärstoffproduktion und physiologische Charakterisierung von marinen heterotrophen Bakterien aus dem deutschen Wattenmeer
… were screened for polyketide synthase-, FADH2-halogenase- and non-ribosomal peptide synthetase genes. Additionally agar diffusion assays were applied to investigate the antagonistic activity of the strains, and some bacteria were tested for acylated homoserine lactones (AHL) production. Three …
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Mechanistic Studies of Lantibiotic Biosynthetic Enzymes and Discovery of Novel Lanthionine-Containing Peptides
… from several bacterial strains and a halogenase involved in tryptophan chlorination.
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Computational Investigation of the Catalytic and Structural Roles of Metals in Metalloenzymes
… halogenation catalyzed by non-heme iron halogenases. Demonstrating the utility of this protocol, our simulations provide essential insights on the interplay between strategic substrate positioning, active-site configurational isomerization, and protein dynamics in halogenases SyrB2, WelO5 …
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Understanding and manipulating alkaloid biosynthesis
… also describe efforts to reengineer tryptophan halogenase RebH to preferentially install chlorine onto tryptamine, the direct indole precursor for the MIAs. After reengineering RebH, we then overexpressed the tryptamine-specific mutant RebH Y455W and flavin reductase RebF in C. roseus and …
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Mechanistic and synthetic studies on the prochlorosin and cytolysin families of lanthipeptides
… that this motif is not recognized by the halogenase MibH, and that the C terminus of microbisporicin is necessary for the chlorination by MibH. During my graduate studies, I had the opportunity to collaborate in a different area of research in our laboratory, the phosphonates. My efforts …
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Elucidating the Biosynthesis of Nucleocidin, a Fluorinated Natural Product Produced by Streptomyces Calvus
… by incorporating the putative ascamycin halogenase genes acmX and acmY into S. calvus Spore 4. Integration of acmX into the chromosome of S. calvus Spore 4 was successful as confirmed by PCR, while integration of acmY could not be confirmed.The presence of nucleocidin in the extracts of …
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Post-translational modifications catalyzed by RiPP and RiPP-adjacent enzymes
… of a remarkably effective flavin-dependent Trp halogenase, ChlH. ChlH normally functions in the biosynthesis of the lasso peptide chlorolassin, in which two of three Trp residues in the final product are modified. Much to our surprise, ChlH modified the linear precursor peptide rather than the …
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Development and characterization of anti-EGFR protein-drug conjugates for tumor therapy
… of ADCs. A putative MibH-like peptidyl halogenase was identified by phylogenetic relationship analysis. Also, expression of the human lysyl oxidase-like 2 (hLOXL2) and the amine oxidase from _Escherichia coli_ (ECAO) was established, which can be used for the scanning of possible …
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Characterizing peptide-modifying enzyme activity in RiPP biosynthesis
… first chapter examines ChlH, a flavin-dependent halogenase from the chlorolassin lasso peptide biosynthetic pathway. ChlH chlorinates tryptophan residues at internal and terminal positions of an assortment of tested peptide substrates. Systematic mutagenesis of its native substrate peptide …