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Showing 1 to 16 of 16 for “"Haloferax volcanii"”.
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Isolation and characterization of motility-defective mutants of Haloferax volcanii.
Haloferax volcanii is a motile extreme halophile, and a detailed physical map of its genome exists. An effort is underway to locate more genes and to increase the detail of this map. Flagellated motile bacteria possess a number of filaments that function as cellular propellers. Motile bacteria swim …
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Discovery of Enzymes Responsible for an Alternate Mevalonate Pathway in Haloferax volcanii
… responsible for the mevalonate pathway in Haloferax volcanii, open-reading frames from the H. volcanii genome were candidate screened against bacterial and eukaryotic mevalonate pathway enzymes and overexpressed in a Haloferax host. This approach has revealed that H. volcanii encodes …
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Cell-cell signaling in archaea: How Haloferax volcanii talk to each other
… of microbial communication. The model archaeon Haloferax volcanii displays distinct morphologies across population densities: cells in culture at early-log phase are rod-shaped while cells in late-log phase are pleomorphic disks. Our studies reveal that applying cell-free conditioned medium (CM) …
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Use of bgaH as a reporter gene for studying translation initiation in the archaeon Haloferax volcanii
The bgaH gene isolated from Haloferax lucentensis codes for P-galactosidase. To study the function of initiator tRNAs in translation initiation in Haloferax volcanii, the initiator AUG codon of the bgaH gene was mutated to UAG, UAA, UGA, and GUC. Four different H. volcanii initiator tRNA derived …
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Bioreactor Janssand : fermentation processes in tidal-flat sediments of the German North Sea
… Studie wurde unmarkiertes Zellmaterial von Haloferax volcanii und Saccharomyces cerevisiae verwendet. Im zweiten und dritten Teil wurden 13C-markierte Substrate verwendet, um die aktiv fermentierenden Bakterien mittels SIP zu identifizieren. Im zweiten Teil wurde Glucose, im dritten Teil …
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Ancient Triassic Halite as a novel source of antibiotic chemical diversity
… The most potent haloarchaea extract was from Haloferax volcanii DS2 with a MIC of 0.5 mg/mL against E. faecium. The yeast-like fungus, A. pullulans EXF-9635, displayed the most potent antimicrobial activity at 0.125 mg/mL against MRSA.<br/><br/>Furthermore, this extract exhibited anti-biofilm …
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A TALE OF TWO METHYLATION MODIFICATIONS IN ARCHAEAL RNAs
… encoding gene, HVO_1989, was deleted from the Haloferax volcanii genome. Analyses of modified base contents indicated that while m1Ø was present in tRNA extracted from the wild-type strain, it was absent from tRNA extracted from the mutant strain. Expression of the gene encoding COG1901 from …
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Novel Cell Surface Anchoring Mechanism Of Prokaryotic Secreted Protein
… acids. Using the well-studied model archaeon Haloferax volcanii S-layer glycoprotein (SLG), previously thought to be anchored in the membrane via its C-terminal hydrophobic domain, I have shown that its H-domain instead is processed and that this processing is dependent on a novel enzyme, the …
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GUIDE RNA-DEPENDENT AND INDEPENDENT tRNA MODIFICATIONS IN ARCHAEA
… stem. In the halophilic euryarchaeon <italic>Haloferax volcanii</italic>, tRNA<super>Trp</super> and tRNA<super>Met</super>, both of which are transcribed as intron-containing pre-tRNA forms, contain Cm34 and ψ54, in addition to other modifications, in these two regions, respectively. The …
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STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF ARCHAEAL BOX H/ACA RIBONUCLEOPROTEIN INVOLVED IN RIBOSOMAL RNA PSEUDOURIDYLATION
… one in helix 90. We successfully deleted Cbf5 in Haloferax volcanii, a haloarchaeon, and showed that the deleted strain was viable. It was the first report where Cbf5 deletion was achieved, because deletion or mutation of cbf5 or of its homologs is lethal in eukaryotes. We also found that the cbf5 …
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Investigating the Biological Role and Binding Modes of Histone-Like Proteins of Halophilic Archaea
… histone gene of another model halophile: hstA of Haloferax volcanii, could be deleted, and that knockout cells remained viable. The genome-wide binding of both halophilic histones was studied, and compared with publicly available data regarding the binding patterns from transcription factors …
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STRUCTURAL AND FUNCTIONAL STUDIES OF ARCHAEAL BOX C/D GUIDE RNA AND ROLE OF A PUTATIVE HUMAN PSEUDOURIDINE SYNTHASE, PUS10 IN APOPTOSIS
… complex. The presence of a unique box C/D RNA of Haloferax volcanii, called sR-tMet has been reported previously to guide the 2’-O-methylation of C34 in elongator pre-tRNAMet. Here we tried to characterize the structure-function relationship of this guide RNA under in vivo conditions. This RNA …