Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Hairy Root Cultures"”.
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Genetic transformation of Ceratotheca triloba for the production of anthraquinones from hairy root cultures
Submitted in complete fulfilment for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2012.
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Bioreactor design for scaleup of Catharanthus roseus hairy root cultures for production of indole alkaloids
… indole alkaloids, vincristine and vinblastine. Hairy root cultures are an attractive alternative for the production of plant derived secondary metabolites. C. roseus hairy root cultures were established in our laboratory by A. rhizogenes mediated transformation of C. roseus seedlings prior to …
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Transformation of Gossypium hirsutum and Gossypium barbadense by Rhizobium rhizogenes: Hairy root induction and gossypol production
… are produced in the leaves, seeds, stems, and roots of cotton plants. Although gossypol is toxic to many animals, other uses for gossypol are actively being investigated. To develop an experimental model for gossypol biosynthesis, a project to produce hairy root cultures from cotton was …
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Production of diospyrin by Euclea natalensis seedlings and in vitro cultures
… potential use of E. natalensis seedlings, callus cultures and hairy root cultures as alternative sources of diospyrin. Chloroform extracts of E. natalensis seedling organs showed antimycobacterial activity against Mycobacterium aurum, and HPLC analysis of the extracts demonstrated a corresponding …
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Phytoecdysteroid accumulation in plants and bioactivities in animal models
… models. Results showed that A. turkestanica hairy root cultures were a sustainable alternative source of valued phytochemicals compared to wild-harvesting (Chapter 2). Extracts from both wild-harvested A. turkestanica shoots and hairy root cultures enhanced protein synthesis in murine cell …
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Isotopic labeling of isoflavones from plant cell and organ culture for metabolic tracking in animal models
… fraction from a high isoflavone yielding kudzu hairy root genotype in vitro, which was used to track the absorption, distribution, and elimination of kudzu isoflavones in rats. Pharmacokinetics showed that kudzu isoflavones were rapidly absorbed, distributed into all tissues including bones, …
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Expression strategies for plant-based production of a vaccine adjuvant
… also confirmed using transient transformation of hairy root cultures. Future experiments will address the practical use of the tobacco-synthesized ricin as adjuvant, as well as the expression of the ricin B subunit fused to a protective antigen of Entamoeba histolytica in tobacco as edible vaccine.
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Metabolic, biochemical and molecular profiling of Catharanthus roseus flower cultivars and transformed hairy roots for monoterpenoid indole alkaloid accumulation /
… procedure. Alternatively, CatharanthllS tissue cultures have been investigated as a source of these agents; however they do not produce vindoline, which is an obligate precursor to vinblastine and vincristine. The interest in developing high MIA cultivars of Catharantlws rosellS has prompted …
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Investigation of Protein-Protein Interactions among Nicotine Biosynthetic Enzymes and Characterization of a Nicotine Transporter
… of nicotine occurs exclusively in the roots of tobacco, yet accumulates in the leaves of tobacco where it is acts as a defense compound to deter insect herbivory. The research detailed in this dissertation addresses two aspects of nicotine physiology in tobacco: 1) an investigation of …
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Plant-derived Murine IL-12 and Ricin B-Murine IL-12 Fusions
… form of mouse IL-12 (mIL-12) was utilized. Hairy root cultures, as a fast-growing bioproduction system were developed from high expressers of mIL-12. A purification scheme was developed to purify plant-derived mIL-12 from hairy roots and purified mIL-12 was used to assess IL-12 bioactivity …